Immobilized mixed-culture reactor (IMcR) for hydrogen and methane production from glucose

Ibdal Satar, Wan Ramli Wan Daud, Byung Hong Kim, Mahendra Rao Somalu, Mostafa Ghasemi

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Immobilized cell technology is a new technique to produce biogas. In the present study, an immobilized mixed-culture reactor (IMcR) in batch-mode operation was used for the production of hydrogen and methane simultaneously from glucose. Several factors, such as glucose concentration, temperature and fermentation time, were evaluated to determine the optimal conditions for hydrogen and methane production. Gas chromatography with a thermal conductivity detector (GC-TCD) and high-performance liquid chromatography (HPLC) were used to analyse the gas and effluent. The morphologies of the immobilized cells were characterized using scanning electron microscopy (SEM). The optimal conditions for hydrogen and methane production were obtained using a substrate with 5.0 g/L glucose at 60 °C for fermentation times of 48.0 h (hydrogen) and 72.0 h (methane). The maximum yields of hydrogen and methane at these optimal conditions were 37.0 ± 0.0 (×10−3) mol/mol glu and 39.0 ± 0.0 (×10−3) mol/mol glu, respectively. The chemical oxygen demand (COD) and pH gradually decreased with increasing fermentation time and temperature. However, the performance of the IMcR decreased over time due to cell damage and microorganism detachment from the cell. In conclusion, the IMcR system is a potential system for the simultaneous production of hydrogen and methane.

Original languageEnglish
Pages (from-to)1188-1196
Number of pages9
JournalEnergy
Volume139
DOIs
Publication statusPublished - 15 Nov 2017

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Glucose
Methane
Hydrogen
Fermentation
Cells
Chemical oxygen demand
Biogas
High performance liquid chromatography
Gas chromatography
Microorganisms
Effluents
Thermal conductivity
Detectors
Temperature
Scanning electron microscopy
Substrates
Gases

Keywords

  • Fermentation time
  • Glucose
  • Hydrogen
  • Immobilized mixed-culture reactor
  • Methane

ASJC Scopus subject areas

  • Pollution
  • Energy(all)

Cite this

Immobilized mixed-culture reactor (IMcR) for hydrogen and methane production from glucose. / Satar, Ibdal; Wan Daud, Wan Ramli; Kim, Byung Hong; Somalu, Mahendra Rao; Ghasemi, Mostafa.

In: Energy, Vol. 139, 15.11.2017, p. 1188-1196.

Research output: Contribution to journalArticle

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