Pemegunan tyrosinase dalam filem komposit selulosa nanokristalin/kitosan untuk pengesanan amperometrik bagi fenol

Translated title of the contribution: Immobilization of tyrosinase in nanocrystalline cellulose/chitosan composite film for amperometric detection of phenol

Fariza Aina Abd Manan, Jaafar Abdullah, Nur Nadziera Nazri, Izyan Nadira Abd Malik, Nor Azah Yusof, Ishak Ahmad

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Nanocrystalline cellulose (NCC)/chitosan composite for immobilization of tyrosinase enzyme for the determination of phenol have been developed. The NCC/Chitosan composite film on screen printed carbon electrode (SPCE) was prepared by using drop casting technique. Characterization of the modified SPCE surface was investigated by using Transmission Electron Microscopy (TEM) and Fourier Transform Infrared (FTIR), respectively. Chronoamperometric (CA) technique is used to perform the electrochemical measurements. The detection of phenols by the developed system is derived on the direct electrochemical reduction of quinones produced by enzymatic reaction. The results demonstrated that the maximum response was observed at ratio of NCC/chitosan of 1 to 1 (v/v), tyrosinase concentration of 10 mg/mL and pH buffer of 7, respectively. It was found that the developed system gave linear response in the phenol concentration range of 0.39-7.74 µM (slope = 28.316, R2 = 0.9808) with the detection limit of 0.38 µM. The reproducibility of the system was also estimated and the Relative Standard Deviation (RSD) was found to be at 4.27%.

Original languageMalay
Pages (from-to)978-985
Number of pages8
JournalMalaysian Journal of Analytical Sciences
Volume20
Issue number5
DOIs
Publication statusPublished - 2016

Fingerprint

Monophenol Monooxygenase
Chitosan
Composite films
Phenol
Cellulose
Carbon
Quinones
Electrodes
Phenols
Fourier transforms
Buffers
Casting
Transmission electron microscopy
Infrared radiation
Composite materials
Enzymes

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Pemegunan tyrosinase dalam filem komposit selulosa nanokristalin/kitosan untuk pengesanan amperometrik bagi fenol. / Manan, Fariza Aina Abd; Abdullah, Jaafar; Nazri, Nur Nadziera; Malik, Izyan Nadira Abd; Yusof, Nor Azah; Ahmad, Ishak.

In: Malaysian Journal of Analytical Sciences, Vol. 20, No. 5, 2016, p. 978-985.

Research output: Contribution to journalArticle

Manan, Fariza Aina Abd ; Abdullah, Jaafar ; Nazri, Nur Nadziera ; Malik, Izyan Nadira Abd ; Yusof, Nor Azah ; Ahmad, Ishak. / Pemegunan tyrosinase dalam filem komposit selulosa nanokristalin/kitosan untuk pengesanan amperometrik bagi fenol. In: Malaysian Journal of Analytical Sciences. 2016 ; Vol. 20, No. 5. pp. 978-985.
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AU - Yusof, Nor Azah

AU - Ahmad, Ishak

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