Immobilization of horseradish peroxidase on β-cyclodextrin-capped silver nanoparticles: Its future aspects in biosensor application

Zoheb Karim, Mohd Jahir Khan, Mohamad Yusof Maskat, Rohana Adnan

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

This study aimed to work out a simple and high-yield procedure for the immobilization of horseradish peroxidase on silver nanoparticle. Ultraviolet–visible (UV-vis) and Fourier-transform infrared spectroscopy and transmission electron microscopy were used to characterize silver nanoparticles. Horseradish peroxidase was immobilized on β-cyclodextrin-capped silver nanoparticles via glutaraldehyde cross-linking. Single-cell gel electrophoresis (Comet assay) was also performed to confirm the genotoxicity of silver nanoparticles. To decrease toxicity, silver nanoparticles were capped with β-cyclodextrin. A comparative stability study of soluble and immobilized enzyme preparations was investigated against pH, temperature, and chaotropic agent, urea. The results showed that the cross-linked peroxidase was significantly more stable as compared to the soluble counterpart. The immobilized enzyme exhibited stable enzyme activities after repeated uses.

Original languageEnglish
Pages (from-to)321-327
Number of pages7
JournalPreparative Biochemistry and Biotechnology
Volume46
Issue number4
DOIs
Publication statusPublished - 18 May 2016

Fingerprint

Cyclodextrins
Biosensing Techniques
Horseradish Peroxidase
Silver
Biosensors
Immobilization
Nanoparticles
Immobilized Enzymes
Comet Assay
Infrared transmission
Enzyme activity
Glutaral
Fourier Transform Infrared Spectroscopy
Electrophoresis
Transmission Electron Microscopy
Peroxidase
Toxicity
Urea
Assays
Gels

Keywords

  • Horseradish peroxidase
  • Immobilization
  • Silver nanoparticle
  • Stability
  • β-cyclodextrin

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology

Cite this

Immobilization of horseradish peroxidase on β-cyclodextrin-capped silver nanoparticles : Its future aspects in biosensor application. / Karim, Zoheb; Khan, Mohd Jahir; Maskat, Mohamad Yusof; Adnan, Rohana.

In: Preparative Biochemistry and Biotechnology, Vol. 46, No. 4, 18.05.2016, p. 321-327.

Research output: Contribution to journalArticle

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