Immobilization of acetylcholinesterase and chromoionophore in a sol-gel matrix for optical biosensor applications

Francis Wong, Musa Ahmad, Yook Heng Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Acetylcholinesterase (AChE) and a pH sensitive chromoionophore were immobilized together in a sol-gel matrix. Using acetylcholine chloride as a substrate, the optical response of the film at wavelengths 635nm and 542 nm were studied. Immobilized of the chromoionophore in sol-gel shows response towards pH change and free enzyme reaction. A calibration curve for the substrate AchE was obtained at a range of 0.073M to 1.006M. A good linearity in the range of 0.26 to 0.87M (R2 = 0.9656) was obtained and the lowest detection limit was 0.26M of AChCl.

Original languageEnglish
Title of host publication2003 Asian Conference on Sensors, AsiaSENSE 2003
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages225-228
Number of pages4
ISBN (Print)0780381017, 9780780381018
DOIs
Publication statusPublished - 2003
Event2003 Asian Conference on Sensors, AsiaSENSE 2003 - Kebangsann, Malaysia
Duration: 18 Jul 2003 → …

Other

Other2003 Asian Conference on Sensors, AsiaSENSE 2003
CountryMalaysia
CityKebangsann
Period18/7/03 → …

Fingerprint

Biosensors
Sol-gels
Substrates
Enzymes
Calibration
Wavelength

Keywords

  • Absorption
  • Biochemistry
  • Biomedical optical imaging
  • Biosensors
  • Calibration
  • Chemical analysis
  • Optical buffering
  • Optical films
  • Optical sensors
  • Substrates

ASJC Scopus subject areas

  • Electrical and Electronic Engineering

Cite this

Wong, F., Ahmad, M., & Lee, Y. H. (2003). Immobilization of acetylcholinesterase and chromoionophore in a sol-gel matrix for optical biosensor applications. In 2003 Asian Conference on Sensors, AsiaSENSE 2003 (pp. 225-228). [1225023] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/ASENSE.2003.1225023

Immobilization of acetylcholinesterase and chromoionophore in a sol-gel matrix for optical biosensor applications. / Wong, Francis; Ahmad, Musa; Lee, Yook Heng.

2003 Asian Conference on Sensors, AsiaSENSE 2003. Institute of Electrical and Electronics Engineers Inc., 2003. p. 225-228 1225023.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Wong, F, Ahmad, M & Lee, YH 2003, Immobilization of acetylcholinesterase and chromoionophore in a sol-gel matrix for optical biosensor applications. in 2003 Asian Conference on Sensors, AsiaSENSE 2003., 1225023, Institute of Electrical and Electronics Engineers Inc., pp. 225-228, 2003 Asian Conference on Sensors, AsiaSENSE 2003, Kebangsann, Malaysia, 18/7/03. https://doi.org/10.1109/ASENSE.2003.1225023
Wong F, Ahmad M, Lee YH. Immobilization of acetylcholinesterase and chromoionophore in a sol-gel matrix for optical biosensor applications. In 2003 Asian Conference on Sensors, AsiaSENSE 2003. Institute of Electrical and Electronics Engineers Inc. 2003. p. 225-228. 1225023 https://doi.org/10.1109/ASENSE.2003.1225023
Wong, Francis ; Ahmad, Musa ; Lee, Yook Heng. / Immobilization of acetylcholinesterase and chromoionophore in a sol-gel matrix for optical biosensor applications. 2003 Asian Conference on Sensors, AsiaSENSE 2003. Institute of Electrical and Electronics Engineers Inc., 2003. pp. 225-228
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