Immobilised sarawak Malaysia yeast cells for production of bioethanol

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Bioethanol production using yeast has become a popular topic due to worrying depleting worldwide fuel reserve. The aim of the study was to investigate the capability of Malaysia yeast strains isolated from starter culture used in traditional fermented food and alcoholic beverages in producing Bioethanol using alginate beads entrapment method. The starter yeast consists of groups of microbes, thus the yeasts were grown in Sabouraud agar to obtain single colony called ST1 (tuak) and ST3 (tapai). The growth in Yeast Potatoes Dextrose (YPD) resulted in specific growth of ST1 at μ = 0.396 h -1 and ST3 at μ = 0.38 h -1, with maximum ethanol production of 7.36 g L -1 observed using ST1 strain. The two strains were then immobilized using calcium alginate entrapment method producing average alginate beads size of 0.51 cm and were grown in different substrates; YPD medium and Local Brown Sugar (LBS) for 8 h in flask. The maximum ethanol concentration measured after 7 h were at 6.63 and 6.59 g L -1 in YPD media and 1.54 and 1.39 g L -1 in LBS media for ST 1 and ST3, respectively. The use of LBS as carbon source showed higher yield of product (Yp/s), 0.59 g g -1 compared to YPD, 0.25 g g -1 in ST1 and (Yp/s), 0.54 g g -1 compared to YPD, 0.24 g g -1 in ST3. This study indicated the possibility of using local strains (ST1 and ST3) to produce bioethanol via immobilization technique with local materials as substrate.

Original languageEnglish
Pages (from-to)526-532
Number of pages7
JournalPakistan Journal of Biological Sciences
Volume14
Issue number9
DOIs
Publication statusPublished - 2011

Fingerprint

ethanol production
Borneo
Malaysia
yeasts
brown sugar
potatoes
cells
glucose
bioethanol
alginates
calcium alginate
alcoholic beverages
fermented foods
traditional foods
starter cultures
agar
methodology
ethanol
microorganisms
carbon

Keywords

  • Bioethanol
  • Brown sugar
  • Calcium alginate
  • Immobilisation
  • Local yeast
  • Yeast fermentation

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

Immobilised sarawak Malaysia yeast cells for production of bioethanol. / Zain, Masniroszaime Mohd; Tan Kofli, Noorhisham; Sheikh Abdullah, Siti Rozaimah.

In: Pakistan Journal of Biological Sciences, Vol. 14, No. 9, 2011, p. 526-532.

Research output: Contribution to journalArticle

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