Immature reticulocyte fraction in guiding stem cell harvest in autologous peripheral blood stem cell transplant

M. N. Sabariah, Leong Chooi Fun, S. K. Cheong

Research output: Contribution to journalArticle

Abstract

Peripheral blood (PB) CD34+ cells enumeration is currently the most reliable method to guide the timing of stem cell harvest. However, its usage is restricted by being technically challenging, costly, and time-consuming. Immature reticulocyte fraction (IRF) determination, which is simpler and cheaper and has a faster turn-around time, has been proposed for a similar purpose. The purpose of this study is to evaluate the value of IRF in guiding stem cell harvest and examine the correlation between IRF and PB CD34+ cells count. Daily pre-harvest tests, i.e. PB CD34+ cells and IRF from 21 patients scheduled for autologous PBSC transplant were assessed. Stem cells harvests were commenced when the PB CD34+ cell count were more than 10 cell/ul. A total of 205 pre-harvest tests were analysed. Following stem cell mobilisations, both the IRF and PB CD 34+ cell counts rose with a variable pattern. In this study, we observed that the IRF peaks preceded the PB CD34+ count by 2 days. On the day of stem cell harvest, all the peak IRF values were >0.3. The PB CD34+ cell counts correlated with the harvested stem cell yield, whereby r2 = 0.77, p < 0.021. In autologous stem cell mobilisation, we believe that IRF is a useful screening tool to predict the rise of the PB CD34+ cell counts as it is a simple, fast and less costly. An IRF of > 0.3 may be used as a cut-off value for the initiation of PB CD34+ quantification prior to stem cell harvest.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalMalaysian Journal of Medicine and Health Sciences
Volume10
Issue number1
Publication statusPublished - 2014

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Reticulocytes
Stem Cells
Transplants
Blood Cell Count
Blood Cells
Hematopoietic Stem Cell Mobilization
Autografts
Peripheral Blood Stem Cells
Cell Count

Keywords

  • Autologous PBSCT
  • Immature reticulocyte fraction

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Immature reticulocyte fraction in guiding stem cell harvest in autologous peripheral blood stem cell transplant. / Sabariah, M. N.; Chooi Fun, Leong; Cheong, S. K.

In: Malaysian Journal of Medicine and Health Sciences, Vol. 10, No. 1, 2014, p. 1-6.

Research output: Contribution to journalArticle

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