ILLUMINA®TRUSEQ® VS NEBNEXT® SMALL RNA library preparation kit for mirna profiling in persicaria minor

Which better?

Abdul Fatah A Samad, Nazaruddin Muhammad Ali, Ismanizan Ismail

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In plants, a group of non-coding small RNA (sRNA) has been provento be an important player in regulating gene expression that can govern network of genetic systems. The two major classes of sRNA which are very extensively studied through deep sequencing, microRNA (miRNA) and small-interfering RNA (siRNA) classes, are well documented. However, the isolation method of sRNA differs depending on the type of sample. Here, we demonstrate the miRNA library preparation using two different Small RNA Library preparation kit, Illumina®TruSeq® Small RNA Preparation and NEBNext® Multiplex Small RNA Library Preparation kit on a plant rich in secondary metabolite Persicaria minor using recommended protocol. The result show NEBNext® Multiplex Small RNA Library Preparation kit can recover small RNA better than Illumina®TruSeq® Small RNA Preparation kit. Thus, this study recommended NEBNext® Multiplex Small RNA Library Preparation kit for miRNA library preparation on Persicaria minor.

Original languageEnglish
Pages (from-to)131-134
Number of pages4
JournalJurnal Teknologi
Volume77
Issue number24
DOIs
Publication statusPublished - 2015

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RNA
Metabolites
Gene expression

Keywords

  • Illumina®TruSeq® Small RNA Preparation kit
  • MiRNA
  • NEBNext® Multiplex Small RNA Library Preparation kit
  • Next qeneration sequencing
  • Persicaria minor

ASJC Scopus subject areas

  • Engineering(all)

Cite this

ILLUMINA®TRUSEQ® VS NEBNEXT® SMALL RNA library preparation kit for mirna profiling in persicaria minor : Which better? / Samad, Abdul Fatah A; Ali, Nazaruddin Muhammad; Ismail, Ismanizan.

In: Jurnal Teknologi, Vol. 77, No. 24, 2015, p. 131-134.

Research output: Contribution to journalArticle

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