Identification of the major allergens of Indian scad (Decapterus russelli)

Rosmilah Misnan, Shahnaz Murad, Meinir Jones, Graham Taylor, Dinah Rahman, Masita Arip, Noormalin Abdullah, Jamaludin Mohamed

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The purpose of this study was to characterize major allergens of Indian scad (Decapterus russelli) which is among the most commonly consumed fish in Malaysia. Raw and cooked extracts of the fish were prepared. Protein profiles and IgE binding patterns were produced by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting using sera from subjects with fish allergy. The major allergens of the fish were then identified by two-dimensional electrophoresis (2-DE), followed by mass spectrometry of the peptide digests. The SDS-PAGE of the raw extract revealed 27 protein fractions over a wide molecular weight range, while the cooked extract demonstrated only six protein fractions. The 1-DE immunoblotting detected 14 IgE-binding proteins, with a molecular weight range from 90 to < 6.5 kDa. Three protein fractions with molecular weights of ∼51, 46 and 12 kDa were identified as the major allergens of this fish. The ∼12 kDa band was a heat-resistant protein while the ∼51 and 46 kDa proteins were sensitive to heat. The 2-DE gel profile of the raw extract demonstrated >100 distinct protein spots and immunoblotting detected at least 10 different major IgE reactive spots with molecular masses as expected and isoelectric point (pl) values ranging from 4.0 to 7.0. A comparison of the major allergenic spot sequences of the 12 kDa proteins with known protein sequences in databases revealed extensive similarity with fish parvalbumin. In conclusion, this study demonstrated that a parvalbumin which is similar to Gad c 1 is the major allergen of Indian scad. Interestingly, we also detected heat-sensitive proteins as major allergenic components in our fish allergy patients.

Original languageEnglish
Pages (from-to)191-198
Number of pages8
JournalAsian Pacific Journal of Allergy and Immunology
Volume26
Issue number4
Publication statusPublished - 2008

Fingerprint

Allergens
Fishes
Immunoblotting
Galectin 3
Parvalbumins
Proteins
Sodium Dodecyl Sulfate
Polyacrylamide Gel Electrophoresis
Hypersensitivity
Molecular Weight
Protein Databases
Malaysia
Isoelectric Point
Immunoglobulin E
Electrophoresis
Mass Spectrometry
Hot Temperature
Peptides
Serum

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Misnan, R., Murad, S., Jones, M., Taylor, G., Rahman, D., Arip, M., ... Mohamed, J. (2008). Identification of the major allergens of Indian scad (Decapterus russelli). Asian Pacific Journal of Allergy and Immunology, 26(4), 191-198.

Identification of the major allergens of Indian scad (Decapterus russelli). / Misnan, Rosmilah; Murad, Shahnaz; Jones, Meinir; Taylor, Graham; Rahman, Dinah; Arip, Masita; Abdullah, Noormalin; Mohamed, Jamaludin.

In: Asian Pacific Journal of Allergy and Immunology, Vol. 26, No. 4, 2008, p. 191-198.

Research output: Contribution to journalArticle

Misnan, R, Murad, S, Jones, M, Taylor, G, Rahman, D, Arip, M, Abdullah, N & Mohamed, J 2008, 'Identification of the major allergens of Indian scad (Decapterus russelli)', Asian Pacific Journal of Allergy and Immunology, vol. 26, no. 4, pp. 191-198.
Misnan R, Murad S, Jones M, Taylor G, Rahman D, Arip M et al. Identification of the major allergens of Indian scad (Decapterus russelli). Asian Pacific Journal of Allergy and Immunology. 2008;26(4):191-198.
Misnan, Rosmilah ; Murad, Shahnaz ; Jones, Meinir ; Taylor, Graham ; Rahman, Dinah ; Arip, Masita ; Abdullah, Noormalin ; Mohamed, Jamaludin. / Identification of the major allergens of Indian scad (Decapterus russelli). In: Asian Pacific Journal of Allergy and Immunology. 2008 ; Vol. 26, No. 4. pp. 191-198.
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