Identification of subgingival periodontopathogen in subjects with chronic periodontitis

Kamil Wisam, Al Bayati Lina, Suhaila Muhammad Ali, Arief Ichwan Solachuddin Jauhari, Ing Chia Phang, Zamirah Zainal Abidin, Shahida Mohd Said

Research output: Contribution to journalConference article

Abstract

Objective: The aim of this study was to identify the presence of subgingival periodontopathogen in subjects diagnosed with chronic periodontitis among patients attending International Islamic University Malaysia, Kulliyyah of Dentistry Polyclinic, Kuantan, State of Pahang. Materials and Methods: Fifty systemically healthy Malaysian subjects were enrolled in this study. Thirty of those who diagnosed with chronic periodontitis were recruited for the test group, and 20 subjects were considered the control group based on the absence of clinical attachment loss. The clinical periodontal parameters including full mouth plaque scores, bleeding on probing, pocket depth and clinical attachment loss were recorded. Subgingival plaque samples were collected using periodontal curette from deep pockets of each individual and pooled for analysis and DNA extraction. The presence of Porphyromonas gingivalis (Pg) was assessed by polymerase chain reaction. Result: There was highly significant difference between both groups in respect to the clinical periodontal parameters (p < 0.001). The prevalence of Pg was 66 % in the study sample with significant increase (p =0.002) in the test group 83.33 % compared to control 40%. Among the chronic periodontitis subjects, the presence of Pg is associated with the increased probing pocket depth (p =0.007). Conclusion: The presence of Pg is significantly detected in chronic periodontitis compared to individuals without periodontal destruction.

Original languageEnglish
Pages (from-to)2285-2291
Number of pages7
JournalMaterials Today: Proceedings
Volume16
DOIs
Publication statusPublished - 1 Jan 2019
Event2017 Conference on Biomedical and Advanced Materials, Bio-CAM 2017 - Langkawi, Malaysia
Duration: 28 Nov 201729 Nov 2017

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Dentistry
Polymerase chain reaction
DNA

Keywords

  • P.gingivali
  • Periodontitis subgingival plaque

ASJC Scopus subject areas

  • Materials Science(all)

Cite this

Identification of subgingival periodontopathogen in subjects with chronic periodontitis. / Wisam, Kamil; Lina, Al Bayati; Ali, Suhaila Muhammad; Solachuddin Jauhari, Arief Ichwan; Phang, Ing Chia; Abidin, Zamirah Zainal; Said, Shahida Mohd.

In: Materials Today: Proceedings, Vol. 16, 01.01.2019, p. 2285-2291.

Research output: Contribution to journalConference article

Wisam, Kamil ; Lina, Al Bayati ; Ali, Suhaila Muhammad ; Solachuddin Jauhari, Arief Ichwan ; Phang, Ing Chia ; Abidin, Zamirah Zainal ; Said, Shahida Mohd. / Identification of subgingival periodontopathogen in subjects with chronic periodontitis. In: Materials Today: Proceedings. 2019 ; Vol. 16. pp. 2285-2291.
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