Identification of sRNA mediated responses to nutrient depletion in Burkholderia pseudomallei

Hirzahida Mohd-Padil, Nadzirah Damiri, Suhaila Sulaiman, Shiao Fei Chai, Sheila Nathan, Mohd Firdaus Mohd Raih

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The Burkholderia genus includes many species that are known to survive in diverse environmental conditions including low nutrient environments. One species, Burkholderia pseudomallei is a versatile pathogen that can survive in a wide range of hosts and environmental conditions. In this study, we investigated how a nutrient depleted growth environment evokes sRNA mediated responses by B. pseudomallei. Computationally predicted B. pseudomallei D286 sRNAs were mapped to RNA-sequencing data for cultures grown under two conditions: (1) BHIB as a nutrient rich media reference environment and (2) M9 media as a nutrient depleted stress environment. The sRNAs were further selected to identify potentially cis-encoded systems by investigating their possible interactions with their flanking genes. The mappings of predicted sRNA genes and interactions analysis to their flanking genes identified 12 sRNA candidates that may possibly have cis-acting regulatory roles that are associated to a nutrient depleted growth environment. Our approach can be used for identifying novel sRNA genes and their possible role as cis-mediated regulatory systems.

Original languageEnglish
Article number17173
JournalScientific Reports
Volume7
Issue number1
DOIs
Publication statusPublished - 1 Dec 2017

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Burkholderia pseudomallei
nutrients
genes
Burkholderia
environmental factors
host range
sequence analysis
pathogens

ASJC Scopus subject areas

  • General

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Identification of sRNA mediated responses to nutrient depletion in Burkholderia pseudomallei. / Mohd-Padil, Hirzahida; Damiri, Nadzirah; Sulaiman, Suhaila; Chai, Shiao Fei; Nathan, Sheila; Mohd Raih, Mohd Firdaus.

In: Scientific Reports, Vol. 7, No. 1, 17173, 01.12.2017.

Research output: Contribution to journalArticle

Mohd-Padil, Hirzahida ; Damiri, Nadzirah ; Sulaiman, Suhaila ; Chai, Shiao Fei ; Nathan, Sheila ; Mohd Raih, Mohd Firdaus. / Identification of sRNA mediated responses to nutrient depletion in Burkholderia pseudomallei. In: Scientific Reports. 2017 ; Vol. 7, No. 1.
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