Identification of significant medium components that affect docosahexaenoic acid production by Schizochytrium sp. SW1

Vidyah Manikan, Aidil Abdul Hamid

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Central composite design (CCD) was employed to investigate the significance of glucose, yeast extract, MSG and sea salt in affecting the amount of docosahexaenoic acid (DHA) accumulated by a locally isolated strain of Schizochytrium. Design Expert software was used to construct a set of experiments where each medium component mentioned above was varied over three levels. Cultivation was carried out in 250mL flasks containing 50mL of medium, incubated at 30°C with 200 rpm agitation for 96 hours. ANOVA was conducted to identify the influential factors and the level of their significance where factors that scored a probability value of less than 0.05 were considered significant. The level of influence for each independent variable was also interpreted using perturbation whereas pattern of interaction between the factors were interpreted using interaction plots. This experiment revealed that yeast extract and monosodium glutamate have significant influence on DHA accumulation process by Schizochytrium sp. SW1.

Original languageEnglish
Title of host publicationAIP Conference Proceedings
Pages277-282
Number of pages6
Volume1571
DOIs
Publication statusPublished - 2013
Event2013 UKM Faculty of Science and Technology Post-Graduate Colloquium - Selangor
Duration: 3 Jul 20134 Jul 2013

Other

Other2013 UKM Faculty of Science and Technology Post-Graduate Colloquium
CitySelangor
Period3/7/134/7/13

Fingerprint

yeast
acids
flasks
glutamates
agitation
glucose
plots
interactions
salts
computer programs
perturbation
composite materials

Keywords

  • Docosahexaenoic acid
  • Schizochytrium
  • Significant factors

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

Identification of significant medium components that affect docosahexaenoic acid production by Schizochytrium sp. SW1. / Manikan, Vidyah; Abdul Hamid, Aidil.

AIP Conference Proceedings. Vol. 1571 2013. p. 277-282.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Manikan, V & Abdul Hamid, A 2013, Identification of significant medium components that affect docosahexaenoic acid production by Schizochytrium sp. SW1. in AIP Conference Proceedings. vol. 1571, pp. 277-282, 2013 UKM Faculty of Science and Technology Post-Graduate Colloquium, Selangor, 3/7/13. https://doi.org/10.1063/1.4858668
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