Identification of restriction-modification systems in Clostridium butyricum using bioinformatic tools

Rozieffa Roslan, Peer Mohamed Abdul, Jamaliah Md Jahim

Research output: Contribution to journalArticle

Abstract

Genetic manipulation is an extremely robust method to enhance the yield of major fermentation product. Nevertheless, restriction-modification (RM) system is the main barrier to genetic manipulation, as it prevents foreign DNA transfer into the host. In order to investigate the presence of restriction enzymes homolog in Clostridium butyricum, a comparative analysis was performed on restriction enzyme sequences from REBASE collection against genome and amino acid sequences of C. butyricum using BLAST program. Multiple restriction enzymes homolog with more than 75% query cover were recognised in the genomes. Based on sequence analysis of five strains, Type II RM system is the most abundant (63.16%), Type IV is the second most abundant (21.05%), followed by Type III (10.53%) and Type I (5.26%). By utilising whole genome sequences and restriction enzyme database, we can comprehend the biological aspect of strain-specific RM systems to develop better strategies and techniques in order to overcome the restriction barrier in genetic manipulation.

Original languageEnglish
Pages (from-to)89-100
Number of pages12
JournalMalaysian Applied Biology
Volume47
Issue number5
Publication statusPublished - 1 Nov 2018

Fingerprint

Clostridium butyricum
DNA Restriction-Modification Enzymes
restriction endonucleases
Computational Biology
bioinformatics
genetic engineering
Genome
Enzymes
enzymes
genome
transfer DNA
Fermentation
Sequence Analysis
Amino Acid Sequence
amino acid sequences
sequence analysis
fermentation
Databases
DNA
methodology

Keywords

  • Clostridium
  • Evolution
  • Genetic engineering
  • Genetic manipulation
  • Restriction enzyme

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Identification of restriction-modification systems in Clostridium butyricum using bioinformatic tools. / Roslan, Rozieffa; Abdul, Peer Mohamed; Md Jahim, Jamaliah.

In: Malaysian Applied Biology, Vol. 47, No. 5, 01.11.2018, p. 89-100.

Research output: Contribution to journalArticle

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