Identification of quantitative trait loci for blast resistance in BC2F3 and BC2F5 advanced backcross families of rice

H. A. Rahim, M. A R Bhuiyan, L. S. Lim, K. K. Sabu, A. Saad, M. Azhar, R. Wickneswari

    Research output: Contribution to journalArticle

    Abstract

    Advanced backcross families derived from Oryza sativa cv MR219/O. rufpogon IRGC105491 were utilized for identification of quantitative trait loci (QTL) for blast resistance using simple sequence repeat markers. Two hundred and sixty-one BC2F3 families were used to construct a linkage map, using 87 markers, which covered 2375.2 cM of 12 rice chromosomes, with a mean density of 27.3 cM. The families were evaluated in a greenhouse for resistance to blast disease caused by pathotypes P7.2 and P5.0 of Magnaporthe oryzae. Five QTLs (qBL5.1, qBL5.2, qBL6.1, qBL8.1, and qBL10.1) for pathotype P5.0 and four QTLs (qBL5.3, qBL5.4, qBL7.1, and qBL8.2) for pathotype P7.2 were identified using the BC2F3 families. Another linkage map was also constructed based on 31 BC2F5 families, using 63 SSR markers, which covered 474.9 cM of 9 rice chromosomes, with a mean density of 8.01 cM. Five suggestive QTLs (qBL11.2, qBL11.3, qBL12.1, qBL12.2, qBL12.3) and one putative QTL (qBL2.1) were identified for pathotype P7.2. Also, seven suggestive QTLs (qBL1.1, qBL2.2, qBL4.1, qBL4.2, qBL5.3, qBL8.3, and qBL11.1) were detected for pathotype P5.0. We conclude that there is a non-race-specific resistance spectrum of O. rufpogon against M. oryzae pathotypes.

    Original languageEnglish
    Pages (from-to)3277-3289
    Number of pages13
    JournalGenetics and Molecular Research
    Volume11
    Issue number3
    DOIs
    Publication statusPublished - Jul 2012

    Fingerprint

    Quantitative Trait Loci
    Magnaporthe
    Chromosomes, Human, Pair 12
    Chromosomes, Human, Pair 9
    Microsatellite Repeats
    Oryza

    Keywords

    • Advanced backcross
    • Disease resistance
    • Oryza rufpogon
    • Quantitative trait loci
    • Rice

    ASJC Scopus subject areas

    • Genetics
    • Molecular Biology

    Cite this

    Identification of quantitative trait loci for blast resistance in BC2F3 and BC2F5 advanced backcross families of rice. / Rahim, H. A.; Bhuiyan, M. A R; Lim, L. S.; Sabu, K. K.; Saad, A.; Azhar, M.; Wickneswari, R.

    In: Genetics and Molecular Research, Vol. 11, No. 3, 07.2012, p. 3277-3289.

    Research output: Contribution to journalArticle

    Rahim, H. A. ; Bhuiyan, M. A R ; Lim, L. S. ; Sabu, K. K. ; Saad, A. ; Azhar, M. ; Wickneswari, R. / Identification of quantitative trait loci for blast resistance in BC2F3 and BC2F5 advanced backcross families of rice. In: Genetics and Molecular Research. 2012 ; Vol. 11, No. 3. pp. 3277-3289.
    @article{d4bf36bcd7ae4a41accdb92c81afb2b9,
    title = "Identification of quantitative trait loci for blast resistance in BC2F3 and BC2F5 advanced backcross families of rice",
    abstract = "Advanced backcross families derived from Oryza sativa cv MR219/O. rufpogon IRGC105491 were utilized for identification of quantitative trait loci (QTL) for blast resistance using simple sequence repeat markers. Two hundred and sixty-one BC2F3 families were used to construct a linkage map, using 87 markers, which covered 2375.2 cM of 12 rice chromosomes, with a mean density of 27.3 cM. The families were evaluated in a greenhouse for resistance to blast disease caused by pathotypes P7.2 and P5.0 of Magnaporthe oryzae. Five QTLs (qBL5.1, qBL5.2, qBL6.1, qBL8.1, and qBL10.1) for pathotype P5.0 and four QTLs (qBL5.3, qBL5.4, qBL7.1, and qBL8.2) for pathotype P7.2 were identified using the BC2F3 families. Another linkage map was also constructed based on 31 BC2F5 families, using 63 SSR markers, which covered 474.9 cM of 9 rice chromosomes, with a mean density of 8.01 cM. Five suggestive QTLs (qBL11.2, qBL11.3, qBL12.1, qBL12.2, qBL12.3) and one putative QTL (qBL2.1) were identified for pathotype P7.2. Also, seven suggestive QTLs (qBL1.1, qBL2.2, qBL4.1, qBL4.2, qBL5.3, qBL8.3, and qBL11.1) were detected for pathotype P5.0. We conclude that there is a non-race-specific resistance spectrum of O. rufpogon against M. oryzae pathotypes.",
    keywords = "Advanced backcross, Disease resistance, Oryza rufpogon, Quantitative trait loci, Rice",
    author = "Rahim, {H. A.} and Bhuiyan, {M. A R} and Lim, {L. S.} and Sabu, {K. K.} and A. Saad and M. Azhar and R. Wickneswari",
    year = "2012",
    month = "7",
    doi = "http://dx.doi.org/10.4238/2012.September.12.11",
    language = "English",
    volume = "11",
    pages = "3277--3289",
    journal = "Genetics and Molecular Research",
    issn = "1676-5680",
    publisher = "Fundacao de Pesquisas Cientificas de Ribeirao Preto",
    number = "3",

    }

    TY - JOUR

    T1 - Identification of quantitative trait loci for blast resistance in BC2F3 and BC2F5 advanced backcross families of rice

    AU - Rahim, H. A.

    AU - Bhuiyan, M. A R

    AU - Lim, L. S.

    AU - Sabu, K. K.

    AU - Saad, A.

    AU - Azhar, M.

    AU - Wickneswari, R.

    PY - 2012/7

    Y1 - 2012/7

    N2 - Advanced backcross families derived from Oryza sativa cv MR219/O. rufpogon IRGC105491 were utilized for identification of quantitative trait loci (QTL) for blast resistance using simple sequence repeat markers. Two hundred and sixty-one BC2F3 families were used to construct a linkage map, using 87 markers, which covered 2375.2 cM of 12 rice chromosomes, with a mean density of 27.3 cM. The families were evaluated in a greenhouse for resistance to blast disease caused by pathotypes P7.2 and P5.0 of Magnaporthe oryzae. Five QTLs (qBL5.1, qBL5.2, qBL6.1, qBL8.1, and qBL10.1) for pathotype P5.0 and four QTLs (qBL5.3, qBL5.4, qBL7.1, and qBL8.2) for pathotype P7.2 were identified using the BC2F3 families. Another linkage map was also constructed based on 31 BC2F5 families, using 63 SSR markers, which covered 474.9 cM of 9 rice chromosomes, with a mean density of 8.01 cM. Five suggestive QTLs (qBL11.2, qBL11.3, qBL12.1, qBL12.2, qBL12.3) and one putative QTL (qBL2.1) were identified for pathotype P7.2. Also, seven suggestive QTLs (qBL1.1, qBL2.2, qBL4.1, qBL4.2, qBL5.3, qBL8.3, and qBL11.1) were detected for pathotype P5.0. We conclude that there is a non-race-specific resistance spectrum of O. rufpogon against M. oryzae pathotypes.

    AB - Advanced backcross families derived from Oryza sativa cv MR219/O. rufpogon IRGC105491 were utilized for identification of quantitative trait loci (QTL) for blast resistance using simple sequence repeat markers. Two hundred and sixty-one BC2F3 families were used to construct a linkage map, using 87 markers, which covered 2375.2 cM of 12 rice chromosomes, with a mean density of 27.3 cM. The families were evaluated in a greenhouse for resistance to blast disease caused by pathotypes P7.2 and P5.0 of Magnaporthe oryzae. Five QTLs (qBL5.1, qBL5.2, qBL6.1, qBL8.1, and qBL10.1) for pathotype P5.0 and four QTLs (qBL5.3, qBL5.4, qBL7.1, and qBL8.2) for pathotype P7.2 were identified using the BC2F3 families. Another linkage map was also constructed based on 31 BC2F5 families, using 63 SSR markers, which covered 474.9 cM of 9 rice chromosomes, with a mean density of 8.01 cM. Five suggestive QTLs (qBL11.2, qBL11.3, qBL12.1, qBL12.2, qBL12.3) and one putative QTL (qBL2.1) were identified for pathotype P7.2. Also, seven suggestive QTLs (qBL1.1, qBL2.2, qBL4.1, qBL4.2, qBL5.3, qBL8.3, and qBL11.1) were detected for pathotype P5.0. We conclude that there is a non-race-specific resistance spectrum of O. rufpogon against M. oryzae pathotypes.

    KW - Advanced backcross

    KW - Disease resistance

    KW - Oryza rufpogon

    KW - Quantitative trait loci

    KW - Rice

    UR - http://www.scopus.com/inward/record.url?scp=84872975588&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=84872975588&partnerID=8YFLogxK

    U2 - http://dx.doi.org/10.4238/2012.September.12.11

    DO - http://dx.doi.org/10.4238/2012.September.12.11

    M3 - Article

    VL - 11

    SP - 3277

    EP - 3289

    JO - Genetics and Molecular Research

    JF - Genetics and Molecular Research

    SN - 1676-5680

    IS - 3

    ER -