Identification of quantitative trait loci for blast resistance in BC<inf>2</inf>F<inf>3</inf> and BC<inf>2</inf>F<inf>5</inf> advanced backcross families of rice

H. A. Rahim, M. A R Bhuiyan, L. S. Lim, K. K. Sabu, A. Saad, M. Azhar, R Wickneswari V Ratnam

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Advanced backcross families derived from Oryza sativa cv MR219/O. rufpogon IRGC105491 were utilized for identification of quantitative trait loci (QTL) for blast resistance using simple sequence repeat markers. Two hundred and sixty-one BC<inf>2</inf>F<inf>3</inf> families were used to construct a linkage map, using 87 markers, which covered 2375.2 cM of 12 rice chromosomes, with a mean density of 27.3 cM. The families were evaluated in a greenhouse for resistance to blast disease caused by pathotypes P7.2 and P5.0 of Magnaporthe oryzae. Five QTLs (qBL5.1, qBL5.2, qBL6.1, qBL8.1, and qBL10.1) for pathotype P5.0 and four QTLs (qBL5.3, qBL5.4, qBL7.1, and qBL8.2) for pathotype P7.2 were identified using the BC<inf>2</inf>F<inf>3</inf> families. Another linkage map was also constructed based on 31 BC<inf>2</inf>F<inf>5</inf> families, using 63 SSR markers, which covered 474.9 cM of 9 rice chromosomes, with a mean density of 8.01 cM. Five suggestive QTLs (qBL11.2, qBL11.3, qBL12.1, qBL12.2, qBL12.3) and one putative QTL (qBL2.1) were identified for pathotype P7.2. Also, seven suggestive QTLs (qBL1.1, qBL2.2, qBL4.1, qBL4.2, qBL5.3, qBL8.3, and qBL11.1) were detected for pathotype P5.0. We conclude that there is a non-race-specific resistance spectrum of O. rufpogon against M. oryzae pathotypes.

Original languageEnglish
Pages (from-to)3277-3289
Number of pages13
JournalGenetics and Molecular Research
Volume11
Issue number3
DOIs
Publication statusPublished - 2012

Fingerprint

Quantitative Trait Loci
Magnaporthe
Chromosomes, Human, Pair 12
Chromosomes, Human, Pair 9
Microsatellite Repeats
Oryza

Keywords

  • Advanced backcross
  • Disease resistance
  • Oryza rufpogon
  • Quantitative trait loci
  • Rice

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

Cite this

Identification of quantitative trait loci for blast resistance in BC<inf>2</inf>F<inf>3</inf> and BC<inf>2</inf>F<inf>5</inf> advanced backcross families of rice. / Rahim, H. A.; Bhuiyan, M. A R; Lim, L. S.; Sabu, K. K.; Saad, A.; Azhar, M.; V Ratnam, R Wickneswari.

In: Genetics and Molecular Research, Vol. 11, No. 3, 2012, p. 3277-3289.

Research output: Contribution to journalArticle

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AU - Bhuiyan, M. A R

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AU - Sabu, K. K.

AU - Saad, A.

AU - Azhar, M.

AU - V Ratnam, R Wickneswari

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