Pangenalpastian protein membran putatif dalam sporozoit Eimeria tenella melalui penyaringan imuno

Translated title of the contribution: Identification of putative membrane proteins in Eimeria tenella sporozoites by immunoscreening

Puay Eng Soon, Fiona M. Tomley, Mohd Sanusi Jangi, Kiew Lian Wan

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In obligate intracellular parasites such as Eimeria tenella, membrane proteins are believed to play an important role in the identification of and attachment to host cell to facilitate host cell invasion. In order to identify proteins on the membrane of E. tenella, an immunoscreening was carried out using antisera against a subcellular fraction which was enriched with membrane proteins from the sporozoites. This immunoscreening effort successfully isolated 21 positive clones. From this total, 13 clones showed significant matches with sequences in the database, i.e. 11 with the microneme protein EtMIC4, one with EtMIC1 and another with EtMIC2. The remaining eight clones which did not show any significant match with sequences in the database was found to carry five different genes. Overall, results of this study demonstrated that the immunoscreening method is capable of identifying novel genes that are likely to code for membrane proteins in E. tenella sporozoites.

Original languageUndefined/Unknown
Pages (from-to)23-28
Number of pages6
JournalSains Malaysiana
Volume35
Issue number2
Publication statusPublished - Dec 2006

Fingerprint

Eimeria tenella
sporozoites
membrane proteins
clones
subcellular fractions
cell invasion
antiserum
genes
proteins
parasites
cells
methodology

Keywords

  • Immunoscreening
  • Membrane protein
  • Sporozoite

ASJC Scopus subject areas

  • General

Cite this

Pangenalpastian protein membran putatif dalam sporozoit Eimeria tenella melalui penyaringan imuno. / Soon, Puay Eng; Tomley, Fiona M.; Jangi, Mohd Sanusi; Wan, Kiew Lian.

In: Sains Malaysiana, Vol. 35, No. 2, 12.2006, p. 23-28.

Research output: Contribution to journalArticle

Soon, Puay Eng ; Tomley, Fiona M. ; Jangi, Mohd Sanusi ; Wan, Kiew Lian. / Pangenalpastian protein membran putatif dalam sporozoit Eimeria tenella melalui penyaringan imuno. In: Sains Malaysiana. 2006 ; Vol. 35, No. 2. pp. 23-28.
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