Identification of potential local isolated for biosurfactant production

Zahra Shafiei, Wan Mohtar Wan Yusoff, Aidil Abdul Hamid, Nasrin Moazami, Ainon Hamzah, Taybeh Fooladi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Biosurfactant are amphiphilic molecule that have received increasing attention in recent years because of their role in the growth of microorganisms on water-insoluble hydrophobic materials such as hydrocarbons as well as their commercial potential in the cosmetics, food, oil recovery and agricultural industries. In this study a potential biosurfactant producing strain was isolated from several soil samples of Terengganu oil refinery, Malaysia and selected during preliminary screening using hemolytic activity, oil spreading and drop collapsed technique. Isolates with at least more than one positive response to these three methods were subjected to complementary screening by measuring surface tension reduction as well as emulsification capacity. The biosurfactant produced by isolated 5M was able to reduced surface tension of culture medium from 60 mN/m to30mN/m. The biochemical and morphological characterization, 16SrRNA gene sequencing showed that the isolated 5M belongs to bacillus groups. The maximum production of biosurfactant by Bacillus 5M was observed after 48 h of incubation.

Original languageEnglish
Title of host publicationAIP Conference Proceedings
Pages191-196
Number of pages6
Volume1571
DOIs
Publication statusPublished - 2013
Event2013 UKM Faculty of Science and Technology Post-Graduate Colloquium - Selangor
Duration: 3 Jul 20134 Jul 2013

Other

Other2013 UKM Faculty of Science and Technology Post-Graduate Colloquium
CitySelangor
Period3/7/134/7/13

Fingerprint

Bacillus
interfacial tension
screening
oils
Malaysia
oil recovery
culture media
sequencing
microorganisms
food
genes
soils
hydrocarbons
industries
water
molecules

Keywords

  • Bacillus licheniformis
  • Biosurfactant
  • Emulsification
  • Local isolate
  • Surface tension

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

Shafiei, Z., Wan Yusoff, W. M., Abdul Hamid, A., Moazami, N., Hamzah, A., & Fooladi, T. (2013). Identification of potential local isolated for biosurfactant production. In AIP Conference Proceedings (Vol. 1571, pp. 191-196) https://doi.org/10.1063/1.4858653

Identification of potential local isolated for biosurfactant production. / Shafiei, Zahra; Wan Yusoff, Wan Mohtar; Abdul Hamid, Aidil; Moazami, Nasrin; Hamzah, Ainon; Fooladi, Taybeh.

AIP Conference Proceedings. Vol. 1571 2013. p. 191-196.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Shafiei, Z, Wan Yusoff, WM, Abdul Hamid, A, Moazami, N, Hamzah, A & Fooladi, T 2013, Identification of potential local isolated for biosurfactant production. in AIP Conference Proceedings. vol. 1571, pp. 191-196, 2013 UKM Faculty of Science and Technology Post-Graduate Colloquium, Selangor, 3/7/13. https://doi.org/10.1063/1.4858653
Shafiei Z, Wan Yusoff WM, Abdul Hamid A, Moazami N, Hamzah A, Fooladi T. Identification of potential local isolated for biosurfactant production. In AIP Conference Proceedings. Vol. 1571. 2013. p. 191-196 https://doi.org/10.1063/1.4858653
Shafiei, Zahra ; Wan Yusoff, Wan Mohtar ; Abdul Hamid, Aidil ; Moazami, Nasrin ; Hamzah, Ainon ; Fooladi, Taybeh. / Identification of potential local isolated for biosurfactant production. AIP Conference Proceedings. Vol. 1571 2013. pp. 191-196
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