Identification of major and minor allergens of black tiger prawn (Penaeus monodon) and king prawn (Penaeus latisulcatus)

Syuhaidah Sahabudin, Rosmilah Misnan, Zailatul Hani Mohammad Yadzir, Jamaludin Mohamed, Noormalin Abdullah, Faizal Bakhtiar, Shahnaz Murad

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Background: Prawns and shrimp are a frequent cause of seafood allergy mediated by IgE antibodies. Penaeus monodon and Penaeus latisulcatus, commonly known as black tiger prawn and king prawn, respectively, are among the most frequently consumed prawns in Malaysia. The aim of this study was to identify the IgE-binding proteins of these 2 prawn species. Methods: Raw and boiled prawn extracts were prepared and then resolved by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). IgE-immunoblotting was then performed using sera from patients with positive skin prick tests to the raw prawn extracts. Results: SDS-PAGE analysis of the raw extracts of both prawn species revealed 23 protein bands; the boiled extracts yielded fewer protein bands. The bands in the range of 40 to 100 kDa were sensitive to heat and therefore were not found in the boiled extracts. Immunoblot of raw extracts of black tiger prawns and king prawns yielded 14 and 11 IgE-binding proteins, respectively, with molecular weights of between 15 and 200 kDa. Proteins at 36, 42, and 49 kDa were detected as the major allergens in both species of prawns. A protein of 75 kDa was also identified as a major allergen in black tiger prawns. Other potential allergens were also observed at various molecular masses. Conclusion: Proteins of 36, 42, and 49 kDa were identified as the major allergens of both species of prawns. The 36 and 42 kDa proteins are hypothesised to be tropomyosin and arginine kinase, respectively. A high molecular weight protein of 75 kDa was found to be an additional major allergen in black tiger prawns.

Original languageEnglish
Pages (from-to)27-32
Number of pages6
JournalMalaysian Journal of Medical Sciences
Volume18
Issue number3
Publication statusPublished - Jul 2011

Fingerprint

Penaeidae
Tigers
Allergens
Proteins
Galectin 3
Sodium Dodecyl Sulfate
Immunoglobulin E
Polyacrylamide Gel Electrophoresis
Arginine Kinase
Molecular Weight
Seafood
Malaysia
Skin Tests
Immunoblotting
Hypersensitivity
Hot Temperature
Antibodies
Serum

Keywords

  • Allergens
  • Allergy and clinical immunology
  • Hypersensitivity
  • Immunoblotting
  • Penaeus
  • Tropomyosin

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Sahabudin, S., Misnan, R., Yadzir, Z. H. M., Mohamed, J., Abdullah, N., Bakhtiar, F., & Murad, S. (2011). Identification of major and minor allergens of black tiger prawn (Penaeus monodon) and king prawn (Penaeus latisulcatus). Malaysian Journal of Medical Sciences, 18(3), 27-32.

Identification of major and minor allergens of black tiger prawn (Penaeus monodon) and king prawn (Penaeus latisulcatus). / Sahabudin, Syuhaidah; Misnan, Rosmilah; Yadzir, Zailatul Hani Mohammad; Mohamed, Jamaludin; Abdullah, Noormalin; Bakhtiar, Faizal; Murad, Shahnaz.

In: Malaysian Journal of Medical Sciences, Vol. 18, No. 3, 07.2011, p. 27-32.

Research output: Contribution to journalArticle

Sahabudin, S, Misnan, R, Yadzir, ZHM, Mohamed, J, Abdullah, N, Bakhtiar, F & Murad, S 2011, 'Identification of major and minor allergens of black tiger prawn (Penaeus monodon) and king prawn (Penaeus latisulcatus)', Malaysian Journal of Medical Sciences, vol. 18, no. 3, pp. 27-32.
Sahabudin, Syuhaidah ; Misnan, Rosmilah ; Yadzir, Zailatul Hani Mohammad ; Mohamed, Jamaludin ; Abdullah, Noormalin ; Bakhtiar, Faizal ; Murad, Shahnaz. / Identification of major and minor allergens of black tiger prawn (Penaeus monodon) and king prawn (Penaeus latisulcatus). In: Malaysian Journal of Medical Sciences. 2011 ; Vol. 18, No. 3. pp. 27-32.
@article{42cf0f39d787457fafd8fb634b36c27d,
title = "Identification of major and minor allergens of black tiger prawn (Penaeus monodon) and king prawn (Penaeus latisulcatus)",
abstract = "Background: Prawns and shrimp are a frequent cause of seafood allergy mediated by IgE antibodies. Penaeus monodon and Penaeus latisulcatus, commonly known as black tiger prawn and king prawn, respectively, are among the most frequently consumed prawns in Malaysia. The aim of this study was to identify the IgE-binding proteins of these 2 prawn species. Methods: Raw and boiled prawn extracts were prepared and then resolved by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). IgE-immunoblotting was then performed using sera from patients with positive skin prick tests to the raw prawn extracts. Results: SDS-PAGE analysis of the raw extracts of both prawn species revealed 23 protein bands; the boiled extracts yielded fewer protein bands. The bands in the range of 40 to 100 kDa were sensitive to heat and therefore were not found in the boiled extracts. Immunoblot of raw extracts of black tiger prawns and king prawns yielded 14 and 11 IgE-binding proteins, respectively, with molecular weights of between 15 and 200 kDa. Proteins at 36, 42, and 49 kDa were detected as the major allergens in both species of prawns. A protein of 75 kDa was also identified as a major allergen in black tiger prawns. Other potential allergens were also observed at various molecular masses. Conclusion: Proteins of 36, 42, and 49 kDa were identified as the major allergens of both species of prawns. The 36 and 42 kDa proteins are hypothesised to be tropomyosin and arginine kinase, respectively. A high molecular weight protein of 75 kDa was found to be an additional major allergen in black tiger prawns.",
keywords = "Allergens, Allergy and clinical immunology, Hypersensitivity, Immunoblotting, Penaeus, Tropomyosin",
author = "Syuhaidah Sahabudin and Rosmilah Misnan and Yadzir, {Zailatul Hani Mohammad} and Jamaludin Mohamed and Noormalin Abdullah and Faizal Bakhtiar and Shahnaz Murad",
year = "2011",
month = "7",
language = "English",
volume = "18",
pages = "27--32",
journal = "Malaysian Journal of Medical Sciences",
issn = "1394-195X",
publisher = "Universiti Sains Malaysia",
number = "3",

}

TY - JOUR

T1 - Identification of major and minor allergens of black tiger prawn (Penaeus monodon) and king prawn (Penaeus latisulcatus)

AU - Sahabudin, Syuhaidah

AU - Misnan, Rosmilah

AU - Yadzir, Zailatul Hani Mohammad

AU - Mohamed, Jamaludin

AU - Abdullah, Noormalin

AU - Bakhtiar, Faizal

AU - Murad, Shahnaz

PY - 2011/7

Y1 - 2011/7

N2 - Background: Prawns and shrimp are a frequent cause of seafood allergy mediated by IgE antibodies. Penaeus monodon and Penaeus latisulcatus, commonly known as black tiger prawn and king prawn, respectively, are among the most frequently consumed prawns in Malaysia. The aim of this study was to identify the IgE-binding proteins of these 2 prawn species. Methods: Raw and boiled prawn extracts were prepared and then resolved by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). IgE-immunoblotting was then performed using sera from patients with positive skin prick tests to the raw prawn extracts. Results: SDS-PAGE analysis of the raw extracts of both prawn species revealed 23 protein bands; the boiled extracts yielded fewer protein bands. The bands in the range of 40 to 100 kDa were sensitive to heat and therefore were not found in the boiled extracts. Immunoblot of raw extracts of black tiger prawns and king prawns yielded 14 and 11 IgE-binding proteins, respectively, with molecular weights of between 15 and 200 kDa. Proteins at 36, 42, and 49 kDa were detected as the major allergens in both species of prawns. A protein of 75 kDa was also identified as a major allergen in black tiger prawns. Other potential allergens were also observed at various molecular masses. Conclusion: Proteins of 36, 42, and 49 kDa were identified as the major allergens of both species of prawns. The 36 and 42 kDa proteins are hypothesised to be tropomyosin and arginine kinase, respectively. A high molecular weight protein of 75 kDa was found to be an additional major allergen in black tiger prawns.

AB - Background: Prawns and shrimp are a frequent cause of seafood allergy mediated by IgE antibodies. Penaeus monodon and Penaeus latisulcatus, commonly known as black tiger prawn and king prawn, respectively, are among the most frequently consumed prawns in Malaysia. The aim of this study was to identify the IgE-binding proteins of these 2 prawn species. Methods: Raw and boiled prawn extracts were prepared and then resolved by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). IgE-immunoblotting was then performed using sera from patients with positive skin prick tests to the raw prawn extracts. Results: SDS-PAGE analysis of the raw extracts of both prawn species revealed 23 protein bands; the boiled extracts yielded fewer protein bands. The bands in the range of 40 to 100 kDa were sensitive to heat and therefore were not found in the boiled extracts. Immunoblot of raw extracts of black tiger prawns and king prawns yielded 14 and 11 IgE-binding proteins, respectively, with molecular weights of between 15 and 200 kDa. Proteins at 36, 42, and 49 kDa were detected as the major allergens in both species of prawns. A protein of 75 kDa was also identified as a major allergen in black tiger prawns. Other potential allergens were also observed at various molecular masses. Conclusion: Proteins of 36, 42, and 49 kDa were identified as the major allergens of both species of prawns. The 36 and 42 kDa proteins are hypothesised to be tropomyosin and arginine kinase, respectively. A high molecular weight protein of 75 kDa was found to be an additional major allergen in black tiger prawns.

KW - Allergens

KW - Allergy and clinical immunology

KW - Hypersensitivity

KW - Immunoblotting

KW - Penaeus

KW - Tropomyosin

UR - http://www.scopus.com/inward/record.url?scp=79960346479&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79960346479&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:79960346479

VL - 18

SP - 27

EP - 32

JO - Malaysian Journal of Medical Sciences

JF - Malaysian Journal of Medical Sciences

SN - 1394-195X

IS - 3

ER -