Identification of genotypes of Influenza A virus in Malaysia

Research output: Contribution to journalArticle

Abstract

Objective: Influenza is considered as an emerging disease until today. The present study was undertaken to determine the prevalent genotypes of Influenza A virus in Malaysia. Methods: Influenza A virus was identified from respiratory specimens by real-time reverse transcriptase polymerase chain reaction (rRT-PCR). Phylogenetic analysis of the identified isolates was performed and genotypes were detected. Results: A total number of 505 throat swabs and nasopharyngeal aspirates were examined by rRT-PCR at Universiti Kebangsaan Malaysia Medical Centre (UKMMC) in which 65(12.87%) were positive for influenza A. The identified isolates were successfully genotyped by phylogenetic analysis. The identified influenza A genotypes were: H1N1 (42), H3N2 (20) and H5N1 (3). Conclusion: The findings indicated that 3 genotypes were circulating in Malaysia during 2011 in which H1N1 was the predominant. Results added new genotype (H5N1) identification record in Malaysia that may be added in data base of WHO and CDC.

Original languageEnglish
JournalPakistan Journal of Medical Sciences
Volume30
Issue number5
DOIs
Publication statusPublished - 2014

Fingerprint

Malaysia
Influenza A virus
Genotype
Human Influenza
Reverse Transcriptase Polymerase Chain Reaction
Centers for Disease Control and Prevention (U.S.)
Pharynx
Real-Time Polymerase Chain Reaction
Databases

Keywords

  • H1N1
  • H3N2
  • H5N1
  • Influenza A
  • Phylogenetic analysis
  • RRT-PCR

ASJC Scopus subject areas

  • Medicine(all)

Cite this

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title = "Identification of genotypes of Influenza A virus in Malaysia",
abstract = "Objective: Influenza is considered as an emerging disease until today. The present study was undertaken to determine the prevalent genotypes of Influenza A virus in Malaysia. Methods: Influenza A virus was identified from respiratory specimens by real-time reverse transcriptase polymerase chain reaction (rRT-PCR). Phylogenetic analysis of the identified isolates was performed and genotypes were detected. Results: A total number of 505 throat swabs and nasopharyngeal aspirates were examined by rRT-PCR at Universiti Kebangsaan Malaysia Medical Centre (UKMMC) in which 65(12.87{\%}) were positive for influenza A. The identified isolates were successfully genotyped by phylogenetic analysis. The identified influenza A genotypes were: H1N1 (42), H3N2 (20) and H5N1 (3). Conclusion: The findings indicated that 3 genotypes were circulating in Malaysia during 2011 in which H1N1 was the predominant. Results added new genotype (H5N1) identification record in Malaysia that may be added in data base of WHO and CDC.",
keywords = "H1N1, H3N2, H5N1, Influenza A, Phylogenetic analysis, RRT-PCR",
author = "Rahman, {Md. Mostafizur} and {Kon Ken}, Wong and I. Isahak and {Zainol Rashid}, {Noor Zetti} and Alfizah Hanafiah",
year = "2014",
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language = "English",
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journal = "Pakistan Journal of Medical Sciences",
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AU - Rahman, Md. Mostafizur

AU - Kon Ken, Wong

AU - Isahak, I.

AU - Zainol Rashid, Noor Zetti

AU - Hanafiah, Alfizah

PY - 2014

Y1 - 2014

N2 - Objective: Influenza is considered as an emerging disease until today. The present study was undertaken to determine the prevalent genotypes of Influenza A virus in Malaysia. Methods: Influenza A virus was identified from respiratory specimens by real-time reverse transcriptase polymerase chain reaction (rRT-PCR). Phylogenetic analysis of the identified isolates was performed and genotypes were detected. Results: A total number of 505 throat swabs and nasopharyngeal aspirates were examined by rRT-PCR at Universiti Kebangsaan Malaysia Medical Centre (UKMMC) in which 65(12.87%) were positive for influenza A. The identified isolates were successfully genotyped by phylogenetic analysis. The identified influenza A genotypes were: H1N1 (42), H3N2 (20) and H5N1 (3). Conclusion: The findings indicated that 3 genotypes were circulating in Malaysia during 2011 in which H1N1 was the predominant. Results added new genotype (H5N1) identification record in Malaysia that may be added in data base of WHO and CDC.

AB - Objective: Influenza is considered as an emerging disease until today. The present study was undertaken to determine the prevalent genotypes of Influenza A virus in Malaysia. Methods: Influenza A virus was identified from respiratory specimens by real-time reverse transcriptase polymerase chain reaction (rRT-PCR). Phylogenetic analysis of the identified isolates was performed and genotypes were detected. Results: A total number of 505 throat swabs and nasopharyngeal aspirates were examined by rRT-PCR at Universiti Kebangsaan Malaysia Medical Centre (UKMMC) in which 65(12.87%) were positive for influenza A. The identified isolates were successfully genotyped by phylogenetic analysis. The identified influenza A genotypes were: H1N1 (42), H3N2 (20) and H5N1 (3). Conclusion: The findings indicated that 3 genotypes were circulating in Malaysia during 2011 in which H1N1 was the predominant. Results added new genotype (H5N1) identification record in Malaysia that may be added in data base of WHO and CDC.

KW - H1N1

KW - H3N2

KW - H5N1

KW - Influenza A

KW - Phylogenetic analysis

KW - RRT-PCR

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