Identification of dominant pathogens in periapical lesions associated with persistent apical periodontitis.

Shuang Zhang, Qian Qian Wang, Cheng Fei Zhang, Eason Soo

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

to identify dominant pathogens in the periapical lesions associated with persistent apical periodontitis. thirty-three root-filled teeth with persistent apical periodontitis referred for surgical treatment were selected. Microbial samples were collected from the periapical lesions during apical surgery. Microbial identification was performed with species-specific primers prepared according to the sequence analysis data using a 16S rRNA technique. among the 33 cases, in 5 cases none of the target species were detected, 6 cases showed the presence of only one species, and 22 cases showed more than two species. Porphyromonas endodontalis (45% of sample) was the most commonly detected dominant microbial species in the study sample, followed by Actinomyces viscosus (42%), Candida albicans (36%) and Porphyromonas gingivalis (27%). Fusobacterium, Actinomyces israelii and Enterococcus faecalis were also detected in 27%, 21% and 15% of the sample, respectively. The most frequently isolated species, P. endodontalis, was in most cases detected together with Actinomyces (14 cases) and P. gingivalis (6 cases). None of the lesions analysed in the present study contained Prevotella intermedia. There was no correlation in relation to the presence of sinus tracts and the bacterial species. a mixed population of pathogens was found in the endodontic lesions associated with persistent apical periodontitis. P. endodontalis, A. viscosus, C. albicans and P. gingivalis were the dominant species identified.

Original languageEnglish
Pages (from-to)115-121
Number of pages7
JournalThe Chinese journal of dental research : the official journal of the Scientific Section of the Chinese Stomatological Association (CSA)
Volume13
Issue number2
Publication statusPublished - 2010

Fingerprint

Porphyromonas endodontalis
Periapical Periodontitis
Porphyromonas gingivalis
Actinomyces viscosus
Actinomyces
Candida albicans
Prevotella intermedia
Fusobacterium
Tooth Root
Enterococcus faecalis
Endodontics
Sequence Analysis
Population

ASJC Scopus subject areas

  • Medicine(all)

Cite this

@article{4e5a4477808c4722afb054100095e5be,
title = "Identification of dominant pathogens in periapical lesions associated with persistent apical periodontitis.",
abstract = "to identify dominant pathogens in the periapical lesions associated with persistent apical periodontitis. thirty-three root-filled teeth with persistent apical periodontitis referred for surgical treatment were selected. Microbial samples were collected from the periapical lesions during apical surgery. Microbial identification was performed with species-specific primers prepared according to the sequence analysis data using a 16S rRNA technique. among the 33 cases, in 5 cases none of the target species were detected, 6 cases showed the presence of only one species, and 22 cases showed more than two species. Porphyromonas endodontalis (45{\%} of sample) was the most commonly detected dominant microbial species in the study sample, followed by Actinomyces viscosus (42{\%}), Candida albicans (36{\%}) and Porphyromonas gingivalis (27{\%}). Fusobacterium, Actinomyces israelii and Enterococcus faecalis were also detected in 27{\%}, 21{\%} and 15{\%} of the sample, respectively. The most frequently isolated species, P. endodontalis, was in most cases detected together with Actinomyces (14 cases) and P. gingivalis (6 cases). None of the lesions analysed in the present study contained Prevotella intermedia. There was no correlation in relation to the presence of sinus tracts and the bacterial species. a mixed population of pathogens was found in the endodontic lesions associated with persistent apical periodontitis. P. endodontalis, A. viscosus, C. albicans and P. gingivalis were the dominant species identified.",
author = "Shuang Zhang and Wang, {Qian Qian} and Zhang, {Cheng Fei} and Eason Soo",
year = "2010",
language = "English",
volume = "13",
pages = "115--121",
journal = "The Chinese journal of dental research : the official journal of the Scientific Section of the Chinese Stomatological Association (CSA)",
issn = "1462-6446",
publisher = "Quintessence Publishing Company",
number = "2",

}

TY - JOUR

T1 - Identification of dominant pathogens in periapical lesions associated with persistent apical periodontitis.

AU - Zhang, Shuang

AU - Wang, Qian Qian

AU - Zhang, Cheng Fei

AU - Soo, Eason

PY - 2010

Y1 - 2010

N2 - to identify dominant pathogens in the periapical lesions associated with persistent apical periodontitis. thirty-three root-filled teeth with persistent apical periodontitis referred for surgical treatment were selected. Microbial samples were collected from the periapical lesions during apical surgery. Microbial identification was performed with species-specific primers prepared according to the sequence analysis data using a 16S rRNA technique. among the 33 cases, in 5 cases none of the target species were detected, 6 cases showed the presence of only one species, and 22 cases showed more than two species. Porphyromonas endodontalis (45% of sample) was the most commonly detected dominant microbial species in the study sample, followed by Actinomyces viscosus (42%), Candida albicans (36%) and Porphyromonas gingivalis (27%). Fusobacterium, Actinomyces israelii and Enterococcus faecalis were also detected in 27%, 21% and 15% of the sample, respectively. The most frequently isolated species, P. endodontalis, was in most cases detected together with Actinomyces (14 cases) and P. gingivalis (6 cases). None of the lesions analysed in the present study contained Prevotella intermedia. There was no correlation in relation to the presence of sinus tracts and the bacterial species. a mixed population of pathogens was found in the endodontic lesions associated with persistent apical periodontitis. P. endodontalis, A. viscosus, C. albicans and P. gingivalis were the dominant species identified.

AB - to identify dominant pathogens in the periapical lesions associated with persistent apical periodontitis. thirty-three root-filled teeth with persistent apical periodontitis referred for surgical treatment were selected. Microbial samples were collected from the periapical lesions during apical surgery. Microbial identification was performed with species-specific primers prepared according to the sequence analysis data using a 16S rRNA technique. among the 33 cases, in 5 cases none of the target species were detected, 6 cases showed the presence of only one species, and 22 cases showed more than two species. Porphyromonas endodontalis (45% of sample) was the most commonly detected dominant microbial species in the study sample, followed by Actinomyces viscosus (42%), Candida albicans (36%) and Porphyromonas gingivalis (27%). Fusobacterium, Actinomyces israelii and Enterococcus faecalis were also detected in 27%, 21% and 15% of the sample, respectively. The most frequently isolated species, P. endodontalis, was in most cases detected together with Actinomyces (14 cases) and P. gingivalis (6 cases). None of the lesions analysed in the present study contained Prevotella intermedia. There was no correlation in relation to the presence of sinus tracts and the bacterial species. a mixed population of pathogens was found in the endodontic lesions associated with persistent apical periodontitis. P. endodontalis, A. viscosus, C. albicans and P. gingivalis were the dominant species identified.

UR - http://www.scopus.com/inward/record.url?scp=79952207498&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79952207498&partnerID=8YFLogxK

M3 - Article

C2 - 21264361

AN - SCOPUS:79952207498

VL - 13

SP - 115

EP - 121

JO - The Chinese journal of dental research : the official journal of the Scientific Section of the Chinese Stomatological Association (CSA)

JF - The Chinese journal of dental research : the official journal of the Scientific Section of the Chinese Stomatological Association (CSA)

SN - 1462-6446

IS - 2

ER -