Identification of differentially expressed circular RNAs in chemoresistant colorectal cancer

Nadiah Abu, Kha Wai Hon, Shivapriya Jeyaraman, Azyani Yahaya, Nik Muhd. Aslan Abdullah, Muaatamarulain Mustangin, Siti A. Sulaiman, A. Rahman A. Jamal, Nurul Syakima Ab Mutalib

Research output: Contribution to journalArticle

Abstract

Aim: Chemoresistance in colorectal cancer (CRC) has become a burden in treating the disease effectively. Circular RNAs (circRNAs) are a type of noncoding RNA that were found to be important in cellular homeostasis. The involvement of circRNAs in relation to chemoresistance in other types of cancers has also been reported. This study aims to identify the differentially expressed circRNAs between chemoresistant and chemosensitive CRC cells. Materials & methods: We developed a chemoresistant cell line model and profiled the circRNAs via microarray. We further validated the expression of two circRNAs in 25 formalin-fixed paraffin-embedded (FFPE) tissue specimens (13 nonresponders and 12 responders) via quantitative polymerase chain reaction (qPCR). Results & conclusion: We found that there were 773 upregulated and 732 downregulated circRNAs between the chemoresistant and chemosensitive HCT-116 cells. We found that hsa-circ-32883 could be a promising biotarget.

Original languageEnglish
Pages (from-to)875-884
Number of pages10
JournalEpigenomics
Volume11
Issue number8
DOIs
Publication statusPublished - 1 Jun 2019

Fingerprint

Colorectal Neoplasms
HCT116 Cells
Untranslated RNA
Paraffin
Formaldehyde
circular RNA
Homeostasis
Down-Regulation
Cell Line
Polymerase Chain Reaction
Neoplasms

Keywords

  • 5-fluorouracil
  • circRNA
  • circRNA-miRNA-mRNA interaction
  • colorectal cancer
  • drug resistance
  • FOLFOX
  • HCT116
  • microarray
  • noncoding RNA
  • oxaliplatin

ASJC Scopus subject areas

  • Genetics
  • Cancer Research

Cite this

Identification of differentially expressed circular RNAs in chemoresistant colorectal cancer. / Abu, Nadiah; Hon, Kha Wai; Jeyaraman, Shivapriya; Yahaya, Azyani; Abdullah, Nik Muhd. Aslan; Mustangin, Muaatamarulain; Sulaiman, Siti A.; A. Jamal, A. Rahman; Ab Mutalib, Nurul Syakima.

In: Epigenomics, Vol. 11, No. 8, 01.06.2019, p. 875-884.

Research output: Contribution to journalArticle

Abu, Nadiah ; Hon, Kha Wai ; Jeyaraman, Shivapriya ; Yahaya, Azyani ; Abdullah, Nik Muhd. Aslan ; Mustangin, Muaatamarulain ; Sulaiman, Siti A. ; A. Jamal, A. Rahman ; Ab Mutalib, Nurul Syakima. / Identification of differentially expressed circular RNAs in chemoresistant colorectal cancer. In: Epigenomics. 2019 ; Vol. 11, No. 8. pp. 875-884.
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AU - A. Jamal, A. Rahman

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