Identification of circulating microRNAs in young men with central obesity

A. Azmir, Norfilza Mohd Mokhtar, S. Norizam, MMN M N Nor Anita, Zaiton Zakaria

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Introduction: Central obesity has been identified as a stronger risk factor than overall obesity for morbidity and mortality of chronic diseases. Currently, studies showed that microRNAs (miRNAs) can be found in adipose tissue and blood circulation of obese persons. Thus, miRNAs can be a potential candidate as a biomarker. Objectives: To determine miRNAs expression in circulating blood of central obese young men compared to non-central obese young men. Significant miRNAs will be validated individually through qPCR. Methods: Sixteen young men with mean age of 32.75±5.93 years old were recruited around Selangor and Kuala Lumpur. The central obese group was comprised of subjects with mean BMI of 31.59±2.92kg/m2, mean waist circumference of 101.92±7.61cm, mean TC of 5.52±1.12mmol/L, mean TG of 1.85±0.74mmol/L, mean HDL of 0.93±0.07mmol/L and mean LDL of 3.75±1.01mmol/L. Meanwhile, the control group was comprised of subjects with mean BMI of 23.86±2.38kg/m2, mean waist circumference of 80.17±3.97cm, mean TC of 4.9±0.33mmol/L, mean TG of 0.78±0.20mmol/L, mean HDL of 1.30±0.15mmol/L and mean LDL of 3.24±0.31mmol/L. The blood was collected for qPCR-based profiling using miRCURY LNA™ Serum/Plasma Focus microRNA PCR Panel. The data were analysed by GenEx software and SPSS version 16. Results & Discussion: Twenty four significant miRNAs were found to be differentially expressed in central obese group compared to normal group (P<0.05). Twenty miRNAs were up-regulated and four miRNAs were down-regulated. Among these, miR-34a has been identified as the most up-regulated miRNA. The aberrantly elevated hepatic miR-34a was found to impair βKL/FGF19 signaling pathway in obesity by targeting directly on βKL mRNA. The role of this miRNA was found in patient with nonalcoholic liver fatty disease and insulin resistance through the FGF19 signaling. Conclusion: This study has identified miRNAs that were differentially expressed in blood circulation of central obese young men. However, these miRNAs must be validated before proceeding to exploratory downstream research.

Original languageEnglish
Pages (from-to)236
Number of pages1
JournalAsian Pacific Journal of Tropical Disease
Volume4
Issue number3
DOIs
Publication statusPublished - Jun 2014

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Abdominal Obesity
MicroRNAs
Blood Circulation
Waist Circumference
Obesity
Disease Resistance
Insulin Resistance
Adipose Tissue
Chronic Disease
Software
Biomarkers

ASJC Scopus subject areas

  • Infectious Diseases
  • Microbiology (medical)

Cite this

Identification of circulating microRNAs in young men with central obesity. / Azmir, A.; Mohd Mokhtar, Norfilza; Norizam, S.; Nor Anita, MMN M N; Zakaria, Zaiton.

In: Asian Pacific Journal of Tropical Disease, Vol. 4, No. 3, 06.2014, p. 236.

Research output: Contribution to journalArticle

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abstract = "Introduction: Central obesity has been identified as a stronger risk factor than overall obesity for morbidity and mortality of chronic diseases. Currently, studies showed that microRNAs (miRNAs) can be found in adipose tissue and blood circulation of obese persons. Thus, miRNAs can be a potential candidate as a biomarker. Objectives: To determine miRNAs expression in circulating blood of central obese young men compared to non-central obese young men. Significant miRNAs will be validated individually through qPCR. Methods: Sixteen young men with mean age of 32.75±5.93 years old were recruited around Selangor and Kuala Lumpur. The central obese group was comprised of subjects with mean BMI of 31.59±2.92kg/m2, mean waist circumference of 101.92±7.61cm, mean TC of 5.52±1.12mmol/L, mean TG of 1.85±0.74mmol/L, mean HDL of 0.93±0.07mmol/L and mean LDL of 3.75±1.01mmol/L. Meanwhile, the control group was comprised of subjects with mean BMI of 23.86±2.38kg/m2, mean waist circumference of 80.17±3.97cm, mean TC of 4.9±0.33mmol/L, mean TG of 0.78±0.20mmol/L, mean HDL of 1.30±0.15mmol/L and mean LDL of 3.24±0.31mmol/L. The blood was collected for qPCR-based profiling using miRCURY LNA™ Serum/Plasma Focus microRNA PCR Panel. The data were analysed by GenEx software and SPSS version 16. Results & Discussion: Twenty four significant miRNAs were found to be differentially expressed in central obese group compared to normal group (P<0.05). Twenty miRNAs were up-regulated and four miRNAs were down-regulated. Among these, miR-34a has been identified as the most up-regulated miRNA. The aberrantly elevated hepatic miR-34a was found to impair βKL/FGF19 signaling pathway in obesity by targeting directly on βKL mRNA. The role of this miRNA was found in patient with nonalcoholic liver fatty disease and insulin resistance through the FGF19 signaling. Conclusion: This study has identified miRNAs that were differentially expressed in blood circulation of central obese young men. However, these miRNAs must be validated before proceeding to exploratory downstream research.",
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T1 - Identification of circulating microRNAs in young men with central obesity

AU - Azmir, A.

AU - Mohd Mokhtar, Norfilza

AU - Norizam, S.

AU - Nor Anita, MMN M N

AU - Zakaria, Zaiton

PY - 2014/6

Y1 - 2014/6

N2 - Introduction: Central obesity has been identified as a stronger risk factor than overall obesity for morbidity and mortality of chronic diseases. Currently, studies showed that microRNAs (miRNAs) can be found in adipose tissue and blood circulation of obese persons. Thus, miRNAs can be a potential candidate as a biomarker. Objectives: To determine miRNAs expression in circulating blood of central obese young men compared to non-central obese young men. Significant miRNAs will be validated individually through qPCR. Methods: Sixteen young men with mean age of 32.75±5.93 years old were recruited around Selangor and Kuala Lumpur. The central obese group was comprised of subjects with mean BMI of 31.59±2.92kg/m2, mean waist circumference of 101.92±7.61cm, mean TC of 5.52±1.12mmol/L, mean TG of 1.85±0.74mmol/L, mean HDL of 0.93±0.07mmol/L and mean LDL of 3.75±1.01mmol/L. Meanwhile, the control group was comprised of subjects with mean BMI of 23.86±2.38kg/m2, mean waist circumference of 80.17±3.97cm, mean TC of 4.9±0.33mmol/L, mean TG of 0.78±0.20mmol/L, mean HDL of 1.30±0.15mmol/L and mean LDL of 3.24±0.31mmol/L. The blood was collected for qPCR-based profiling using miRCURY LNA™ Serum/Plasma Focus microRNA PCR Panel. The data were analysed by GenEx software and SPSS version 16. Results & Discussion: Twenty four significant miRNAs were found to be differentially expressed in central obese group compared to normal group (P<0.05). Twenty miRNAs were up-regulated and four miRNAs were down-regulated. Among these, miR-34a has been identified as the most up-regulated miRNA. The aberrantly elevated hepatic miR-34a was found to impair βKL/FGF19 signaling pathway in obesity by targeting directly on βKL mRNA. The role of this miRNA was found in patient with nonalcoholic liver fatty disease and insulin resistance through the FGF19 signaling. Conclusion: This study has identified miRNAs that were differentially expressed in blood circulation of central obese young men. However, these miRNAs must be validated before proceeding to exploratory downstream research.

AB - Introduction: Central obesity has been identified as a stronger risk factor than overall obesity for morbidity and mortality of chronic diseases. Currently, studies showed that microRNAs (miRNAs) can be found in adipose tissue and blood circulation of obese persons. Thus, miRNAs can be a potential candidate as a biomarker. Objectives: To determine miRNAs expression in circulating blood of central obese young men compared to non-central obese young men. Significant miRNAs will be validated individually through qPCR. Methods: Sixteen young men with mean age of 32.75±5.93 years old were recruited around Selangor and Kuala Lumpur. The central obese group was comprised of subjects with mean BMI of 31.59±2.92kg/m2, mean waist circumference of 101.92±7.61cm, mean TC of 5.52±1.12mmol/L, mean TG of 1.85±0.74mmol/L, mean HDL of 0.93±0.07mmol/L and mean LDL of 3.75±1.01mmol/L. Meanwhile, the control group was comprised of subjects with mean BMI of 23.86±2.38kg/m2, mean waist circumference of 80.17±3.97cm, mean TC of 4.9±0.33mmol/L, mean TG of 0.78±0.20mmol/L, mean HDL of 1.30±0.15mmol/L and mean LDL of 3.24±0.31mmol/L. The blood was collected for qPCR-based profiling using miRCURY LNA™ Serum/Plasma Focus microRNA PCR Panel. The data were analysed by GenEx software and SPSS version 16. Results & Discussion: Twenty four significant miRNAs were found to be differentially expressed in central obese group compared to normal group (P<0.05). Twenty miRNAs were up-regulated and four miRNAs were down-regulated. Among these, miR-34a has been identified as the most up-regulated miRNA. The aberrantly elevated hepatic miR-34a was found to impair βKL/FGF19 signaling pathway in obesity by targeting directly on βKL mRNA. The role of this miRNA was found in patient with nonalcoholic liver fatty disease and insulin resistance through the FGF19 signaling. Conclusion: This study has identified miRNAs that were differentially expressed in blood circulation of central obese young men. However, these miRNAs must be validated before proceeding to exploratory downstream research.

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