Identification of calcium-dependent protein kinase (CDPK): A multi-functional gene family in Rafflesia cantleyi

Safoora Amini, Hoe Han Goh, Kiew Lian Wan

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Rafflesia, a parasitic plant that belongs to the Rafflesiaceae family, is notable for producing the largest flowers in the world. This study focused on identification of Calcium-dependent protein kinases (CDPKs) due to their vital roles in plant growth and development, biotic and abiotic stress responses, and hormone signaling. RNA-seq data generated from three bud stages of Rafflesia cantleyi ie BS1, BS2, and BS3 and were assembled. Based on the BLAST searches of Rafflesia unique transcripts (UTs) to Arabidopsis TAIR database, a total of 14 unique transcripts (UTs) were identified as CDPK1 to CDPK5, CDPK7 to CDPK11, CDPK16, CDPK18, CDPK19, and CDPK28. These genes are expressed at all three bud stages of R. cantleyi with up-regulation pattern at BS1 vs. BS2 and BS2 vs. BS3. This result shows that the expression of CDPK gene family increases by developmental progress in Rafflesia in order to regulate biochemical and molecular changes at the cellular level in response to exposure to environmental changes. However, CDPKs functions in plants growth and defense process still need more experimental evidence to deeply understand their biological roles in R. cantleyi.

Original languageEnglish
Title of host publication2016 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2016 Postgraduate Colloquium
PublisherAmerican Institute of Physics Inc.
Volume1784
ISBN (Electronic)9780735414464
DOIs
Publication statusPublished - 17 Nov 2016
Event2016 Postgraduate Colloquium of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology, UKM FST 2016 - Selangor, Malaysia
Duration: 13 Apr 201614 Apr 2016

Other

Other2016 Postgraduate Colloquium of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology, UKM FST 2016
CountryMalaysia
CitySelangor
Period13/4/1614/4/16

Fingerprint

genes
calcium
proteins
hormones

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

Amini, S., Goh, H. H., & Wan, K. L. (2016). Identification of calcium-dependent protein kinase (CDPK): A multi-functional gene family in Rafflesia cantleyi. In 2016 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2016 Postgraduate Colloquium (Vol. 1784). [020003] American Institute of Physics Inc.. https://doi.org/10.1063/1.4966713

Identification of calcium-dependent protein kinase (CDPK) : A multi-functional gene family in Rafflesia cantleyi. / Amini, Safoora; Goh, Hoe Han; Wan, Kiew Lian.

2016 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2016 Postgraduate Colloquium. Vol. 1784 American Institute of Physics Inc., 2016. 020003.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Amini, S, Goh, HH & Wan, KL 2016, Identification of calcium-dependent protein kinase (CDPK): A multi-functional gene family in Rafflesia cantleyi. in 2016 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2016 Postgraduate Colloquium. vol. 1784, 020003, American Institute of Physics Inc., 2016 Postgraduate Colloquium of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology, UKM FST 2016, Selangor, Malaysia, 13/4/16. https://doi.org/10.1063/1.4966713
Amini S, Goh HH, Wan KL. Identification of calcium-dependent protein kinase (CDPK): A multi-functional gene family in Rafflesia cantleyi. In 2016 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2016 Postgraduate Colloquium. Vol. 1784. American Institute of Physics Inc. 2016. 020003 https://doi.org/10.1063/1.4966713
Amini, Safoora ; Goh, Hoe Han ; Wan, Kiew Lian. / Identification of calcium-dependent protein kinase (CDPK) : A multi-functional gene family in Rafflesia cantleyi. 2016 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2016 Postgraduate Colloquium. Vol. 1784 American Institute of Physics Inc., 2016.
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