Identification of bio-active constituents from Derris elliptica liquid crude extract using liquid chromatographic method coupled with high vacuum pressure

Saiful Irwan Zubairi, Mohamad Roji Sarmidi, Ramlan Abdul Aziz

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

History has recorded that the use of rotenone as bio-pesticide by plant and vegetables growers are almost worldwide. The re-introduction of this bio-active compound as an environmental friendly bio-pesticide in organic fruits and vegetables plantations is now an emergent trend. Currently, our study is focused on the development of bio-pesticide using rotenone extracted from local plant Derris elliptica. Liquid chromatographic method coupled with high vacuum pressure was developed for the purification of rotenone in the crude extract of Derris elliptica to ease identification process. The treated root and stem were cut into small pieces prior to the extraction process using the normal soaking extraction (NSE) method. Next, the liquid crude extract was concentrated by using rotary evaporator at 40 °C under reduced pressure of 800 mbar (90% of solvent removal). A high vacuum pressure liquid chromatographic (VLC) was carried out to purify the crude extract using silica gel with variety of eluents composition. All eluted solutions that have been collected from the reservoir were analyzed by mean of thin layer chromatography (TLC). The markers for each of the bio-active constituents were visualized and calculated using UV lamp and the Rf values respectively. The employed method shows significant improvement in preliminary identification of rotenone and its derivatives as compared to the conventional thin layer chromatography method.

Original languageEnglish
Pages (from-to)437-440
Number of pages4
JournalAdvances in Environmental Biology
Volume8
Issue number2
Publication statusPublished - 2014

Fingerprint

Derris elliptica
Derris
Rotenone
rotenone
Vacuum
Complex Mixtures
Pesticides
Pressure
liquid
pesticides
liquids
pesticide
extracts
Thin Layer Chromatography
thin layer chromatography
Vegetables
vegetable
chromatography
vegetables
evaporators

Keywords

  • Chromatography
  • Derris elliptica
  • Liquid crude extract
  • Rotenone

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Environmental Science(all)

Cite this

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abstract = "History has recorded that the use of rotenone as bio-pesticide by plant and vegetables growers are almost worldwide. The re-introduction of this bio-active compound as an environmental friendly bio-pesticide in organic fruits and vegetables plantations is now an emergent trend. Currently, our study is focused on the development of bio-pesticide using rotenone extracted from local plant Derris elliptica. Liquid chromatographic method coupled with high vacuum pressure was developed for the purification of rotenone in the crude extract of Derris elliptica to ease identification process. The treated root and stem were cut into small pieces prior to the extraction process using the normal soaking extraction (NSE) method. Next, the liquid crude extract was concentrated by using rotary evaporator at 40 °C under reduced pressure of 800 mbar (90{\%} of solvent removal). A high vacuum pressure liquid chromatographic (VLC) was carried out to purify the crude extract using silica gel with variety of eluents composition. All eluted solutions that have been collected from the reservoir were analyzed by mean of thin layer chromatography (TLC). The markers for each of the bio-active constituents were visualized and calculated using UV lamp and the Rf values respectively. The employed method shows significant improvement in preliminary identification of rotenone and its derivatives as compared to the conventional thin layer chromatography method.",
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AU - Aziz, Ramlan Abdul

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AB - History has recorded that the use of rotenone as bio-pesticide by plant and vegetables growers are almost worldwide. The re-introduction of this bio-active compound as an environmental friendly bio-pesticide in organic fruits and vegetables plantations is now an emergent trend. Currently, our study is focused on the development of bio-pesticide using rotenone extracted from local plant Derris elliptica. Liquid chromatographic method coupled with high vacuum pressure was developed for the purification of rotenone in the crude extract of Derris elliptica to ease identification process. The treated root and stem were cut into small pieces prior to the extraction process using the normal soaking extraction (NSE) method. Next, the liquid crude extract was concentrated by using rotary evaporator at 40 °C under reduced pressure of 800 mbar (90% of solvent removal). A high vacuum pressure liquid chromatographic (VLC) was carried out to purify the crude extract using silica gel with variety of eluents composition. All eluted solutions that have been collected from the reservoir were analyzed by mean of thin layer chromatography (TLC). The markers for each of the bio-active constituents were visualized and calculated using UV lamp and the Rf values respectively. The employed method shows significant improvement in preliminary identification of rotenone and its derivatives as compared to the conventional thin layer chromatography method.

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