Identification of Bacillus cereus isolates from cooked rice by biochemical test and 16s rDNA sequences

Nisreen Jawad, Sahilah Abd. Mutalib

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Bacillus cereus isolates are considered to be toxigenic and can lead to food poisoning. Cooked rice is a potentially risky food, particularly while grown in tropical countries. The present study aims to explore the prevalence of B. cereus in cooked rice from several restaurant and stalls in the area (Bangi, Kajang and UKM) in Selangor Malaysia. The isolation was conducted using the standard procedure for detection of B. cereus. Of seventy (n= 70) cooked rice samples examined, 42. 8% were positive for B. cereus indicated bright pink colonies when it grown onto mannitol egg yolk polmyxin medium. The thirty five (35) colonies were selected and identified as B. cereus by biochemical test and 16s rDNA sequences. The results in the presence study, showed the cooked rice samples were potentially can cause food poisoning to the public consumers.

Original languageEnglish
Pages (from-to)469-475
Number of pages7
JournalInternational Journal of ChemTech Research
Volume9
Issue number3
Publication statusPublished - 2016

Fingerprint

Bacillus cereus
Ribosomal DNA
Mannitol

Keywords

  • 16s rDNA sequences
  • Bacillus cereus
  • Biochemical test
  • Cooked rice
  • Identification

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Chemistry(all)

Cite this

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abstract = "Bacillus cereus isolates are considered to be toxigenic and can lead to food poisoning. Cooked rice is a potentially risky food, particularly while grown in tropical countries. The present study aims to explore the prevalence of B. cereus in cooked rice from several restaurant and stalls in the area (Bangi, Kajang and UKM) in Selangor Malaysia. The isolation was conducted using the standard procedure for detection of B. cereus. Of seventy (n= 70) cooked rice samples examined, 42. 8{\%} were positive for B. cereus indicated bright pink colonies when it grown onto mannitol egg yolk polmyxin medium. The thirty five (35) colonies were selected and identified as B. cereus by biochemical test and 16s rDNA sequences. The results in the presence study, showed the cooked rice samples were potentially can cause food poisoning to the public consumers.",
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