Identification and validation of sheath blight resistance in rice (Oryza sativa L.) cultivars against Rhizoctonia solani

Md Kamal Hossain, Ong Shin Tze, Kalaivani K. Nadarajah, Kshirod Jena, Md Atiqur Rahman Bhuiyan, R Wickneswari V Ratnam

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Rice sheath blight, caused by Rhizoctonia solani, is a devastating disease of rice which causes major yield loss in most rice growing regions of the world. Hence, identification and subsequent development of disease resistance in rice cultivars is crucial. Six moderately resistant cultivars, namely Teqing, Jasmine85, Tetep, Pecos, Azucena and Taducan, one susceptible local cultivar, MR 219, and two new advanced breeding lines, UKMRC 2 and UKMRC 9, were screened using micro-chamber and mist-chamber methods. The fungal isolate was confirmed as R. solani using ITS-rDNA sequencing. Severe sheath blight was recorded following inoculation with R. solani under micro-chamber conditions. The most resistant cultivar was Tetep, followed by Teqing. In mist-chamber screening, UKMRC 2 showed the highest level of susceptibility with a disease severity index (DSI) of 6.67, while MR 219 produced the highest DSI of 7.22 in the micro-chamber. Significant correlation of plant height and disease was obtained with relative lesion height (RLH) indices. Significant correlations were also observed among diseased plant affected area (DPAA), VRT (visual rating) and RLH, with VRT being the most accurate. On the basis of the disease reactions, Tetep and Teqing were identified as suitable donors to improve resistance in UKMRC 2 and MR 219. Mist-chamber screening method was more reliable to evaluate sheath blight under greenhouse conditions than the micro-chamber method.

Original languageEnglish
Pages (from-to)482-490
Number of pages9
JournalCanadian Journal of Plant Pathology
Volume36
Issue number4
DOIs
Publication statusPublished - 2 Oct 2014

Fingerprint

Thanatephorus cucumeris
blight
Oryza sativa
rice
lesions (plant)
cultivars
disease severity
screening
breeding lines
disease resistance
methodology
greenhouses

Keywords

  • Disease resistance
  • Rhizoctonia solani
  • rice cultivar
  • screening methods
  • sheath blight

ASJC Scopus subject areas

  • Plant Science
  • Agronomy and Crop Science

Cite this

Identification and validation of sheath blight resistance in rice (Oryza sativa L.) cultivars against Rhizoctonia solani. / Hossain, Md Kamal; Tze, Ong Shin; K. Nadarajah, Kalaivani; Jena, Kshirod; Bhuiyan, Md Atiqur Rahman; V Ratnam, R Wickneswari.

In: Canadian Journal of Plant Pathology, Vol. 36, No. 4, 02.10.2014, p. 482-490.

Research output: Contribution to journalArticle

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