Identification and mode of action of antibacterial components from Melastoma Malabathricum linn leaves

Mourouge Saadi Alwash, Nazlina Ibrahim, Wan Yaacob Wan Ahmad

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The present study aimed to assess the antibacterial activity of the methanol extract of Melastoma malabathricum Leaves (MMML), active fraction of M. malabathricum Leaves (ML5) and isolated kaempferol-3-O-(2,6-di-O-p-trans-coumaroyl)-β-glucopyranoside (Kf) and to describe the preliminary mode of action against Gram-positive and Gram-negative bacteria. The MMML extract was fractionated by Vacuum Liquid Chromatography (VLC) to afford M. malabathricum leaves fractions (ML1-ML6) of increasing polarities. Bioassay-guided fractionation (direct-TLC bioautography) revealed that fraction of M. malabathricum leaves (ML5) had the highest antibacterial activity. Therefore, the ML5 was further subjected to Column Chromatography (CC) to afford Kaempferol-3-O-(2,6-di-O-p-trans-coumaroyl)-β- glucopyranoside (Kf). The compound structure was elucidated using data obtained from 1H NMR and 13C NMR spectra. Antibacterial activity of MMML, ML5 and bioactive constituents Kf against Staphylococcus aureus reference strain, Methicillin-Resistant S. aureus reference strain (MRSA), 11 clinical MRSA isolates, 3 clinical Pseudomonas aeruginosa isolates and P. aeruginosa reference strain was evaluated through disk diffusion method and Minimum Inhibitory Concentration (MIC). The time kill assay and field emission scanning electron microscopy was used to determine the preliminary mode of action. The data obtained from disk diffusion method and MIC showed that the MMML, ML5 and Kf showed antibacterial activity against all test bacteria with various values. Generally, the ML5 and Kf exhibited the highest antibacterial activity with the largest zones of inhibition and lowest MIC values against S. aureus and all tested MRSA. The MMML exerted the strongest killing effect against all tested bacteria while the overall performance of ML5 and Kf were significantly more effective against S. aureus and MRSA than P. aeruginosa. Field emission scanning electron microscopy revealed irregular bacterial surface indicating that the MMML, ML5 and Kf disrupted the integrity of membrane. In conclusion, the results obtained showed that MMML, ML5 and Kf have potential alternative to antibiotics.

Original languageEnglish
Pages (from-to)46-58
Number of pages13
JournalAmerican Journal of Infectious Diseases
Volume9
Issue number2
DOIs
Publication statusPublished - 2013

Fingerprint

Methicillin-Resistant Staphylococcus aureus
Microbial Sensitivity Tests
Pseudomonas aeruginosa
Staphylococcus aureus
Electron Scanning Microscopy
Bacteria
Vacuum
Gram-Negative Bacteria
Liquid Chromatography
Biological Assay
Methanol
Chromatography
Anti-Bacterial Agents
Membranes
kaempferol

Keywords

  • Antibacterial and mode of action
  • Flavonoids
  • Melastoma malabathricum linn leaves

ASJC Scopus subject areas

  • Infectious Diseases

Cite this

Identification and mode of action of antibacterial components from Melastoma Malabathricum linn leaves. / Alwash, Mourouge Saadi; Ibrahim, Nazlina; Wan Ahmad, Wan Yaacob.

In: American Journal of Infectious Diseases, Vol. 9, No. 2, 2013, p. 46-58.

Research output: Contribution to journalArticle

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