Identification and cloning of four riboswitches from Burkholderia pseudomallei strain K96243

Noor Munyati-Othman, Ahmad Luqman Abdul Fatah, Mohd Al Akmarul Fizree Bin Md Piji, Effirul Ikhwan Ramlan, Mohd Firdaus Mohd Raih

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Structured RNAs referred as riboswitches have been predicted to be present in the genome sequence of Burkholderia pseudomallei strain K96243. Four of the riboswitches were identified and analyzed through BLASTN, Rfam search and multiple sequence alignment. The RNA aptamers belong to the following riboswitch classifications: glycine riboswitch, cobalamin riboswitch, S-adenosyl-(L)-homocysteine (SAH) riboswitch and flavin mononucleotide (FMN) riboswitch. The conserved nucleotides for each aptamer were identified and were marked on the secondary structure generated by RNAfold. These riboswitches were successfully amplified and cloned for further study.

Original languageEnglish
Title of host publication2015 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium
PublisherAmerican Institute of Physics Inc.
Volume1678
ISBN (Electronic)9780735413252
DOIs
Publication statusPublished - 25 Sep 2015
Event2015 Postgraduate Colloquium of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology, UKM FST 2015 - Selangor, Malaysia
Duration: 15 Apr 201516 Apr 2015

Other

Other2015 Postgraduate Colloquium of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology, UKM FST 2015
CountryMalaysia
CitySelangor
Period15/4/1516/4/15

Fingerprint

genome
nucleotides
glycine
alignment

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

Munyati-Othman, N., Fatah, A. L. A., Piji, M. A. A. F. B. M., Ramlan, E. I., & Mohd Raih, M. F. (2015). Identification and cloning of four riboswitches from Burkholderia pseudomallei strain K96243. In 2015 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium (Vol. 1678). [030018] American Institute of Physics Inc.. https://doi.org/10.1063/1.4931239

Identification and cloning of four riboswitches from Burkholderia pseudomallei strain K96243. / Munyati-Othman, Noor; Fatah, Ahmad Luqman Abdul; Piji, Mohd Al Akmarul Fizree Bin Md; Ramlan, Effirul Ikhwan; Mohd Raih, Mohd Firdaus.

2015 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium. Vol. 1678 American Institute of Physics Inc., 2015. 030018.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Munyati-Othman, N, Fatah, ALA, Piji, MAAFBM, Ramlan, EI & Mohd Raih, MF 2015, Identification and cloning of four riboswitches from Burkholderia pseudomallei strain K96243. in 2015 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium. vol. 1678, 030018, American Institute of Physics Inc., 2015 Postgraduate Colloquium of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology, UKM FST 2015, Selangor, Malaysia, 15/4/15. https://doi.org/10.1063/1.4931239
Munyati-Othman N, Fatah ALA, Piji MAAFBM, Ramlan EI, Mohd Raih MF. Identification and cloning of four riboswitches from Burkholderia pseudomallei strain K96243. In 2015 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium. Vol. 1678. American Institute of Physics Inc. 2015. 030018 https://doi.org/10.1063/1.4931239
Munyati-Othman, Noor ; Fatah, Ahmad Luqman Abdul ; Piji, Mohd Al Akmarul Fizree Bin Md ; Ramlan, Effirul Ikhwan ; Mohd Raih, Mohd Firdaus. / Identification and cloning of four riboswitches from Burkholderia pseudomallei strain K96243. 2015 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium. Vol. 1678 American Institute of Physics Inc., 2015.
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