Identification and characterization of a mating signalling gene from an oil palm pathogen, Ganoderma boninense

Ahmad Zairun Madihah, Abu Seman Idris, Shazilah Kamaruddin, Izwan Bharudin, Farah Diba Abu Bakar, Abdul Munir Abd. Murad

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Basal stem rot (BSR) disease is a devastating threat to Malaysia oil palm plantation, which is caused by Ganoderma boninense fungi. Dikaryon is the dominant stage of G. boninense and the pathogenic form of the fungus as compared to the monokaryon. Mating signalling response lead by mitogen-activated pathway (MAPK) responsible in the transformation of sterile monokaryon to pathogenic dikaryon. Expression of the STE3 gene involved in the autocrine activation of mating pathway. Mutations of STE3 gene in some pathogenic yeast resulted in inhibition of pheromone responses and blocked the signalling in the cell fusion. Identification of pheromone receptor, STE3 gene involved in mating signalling pathway is important to understand the mating response towards pathogenesis of G. boninense. Genome walking and polymerase chain reaction (PCR) were carried out to amplify isolate the full length of STE3 gene. Several in silico sequence analysis were conducted to characterise the STE3 gene. The full-length gene sequence including the upstream and downstream regions obtained could be used in future studies to understand pathogenesis of G. boninense.

Original languageEnglish
Title of host publication2018 UKM FST Postgraduate Colloquium
Subtitle of host publicationProceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2018 Postgraduate Colloquium
EditorsNoor Hayati Ahmad Rasol, Kamarulzaman Ibrahim, Siti Aishah Hasbullah, Mohammad Hafizuddin Hj. Jumali, Nazlina Ibrahim, Marlia Mohd Hanafiah, Mohd Talib Latif
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735418431
DOIs
Publication statusPublished - 27 Jun 2019
Event2018 UKM FST Postgraduate Colloquium - Selangor, Malaysia
Duration: 4 Apr 20186 Apr 2018

Publication series

NameAIP Conference Proceedings
Volume2111
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference2018 UKM FST Postgraduate Colloquium
CountryMalaysia
CitySelangor
Period4/4/186/4/18

Fingerprint

pathogens
Elaeis guineensis
genes
pathogen
oils
gene
oil
pathogenesis
fungi
genome walking
pheromone
cell fusion
stem rot
chemoreceptors
fungus
Malaysia
pheromones
polymerase chain reaction
walking
sequence analysis

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Ecology
  • Plant Science
  • Physics and Astronomy(all)
  • Nature and Landscape Conservation

Cite this

Madihah, A. Z., Idris, A. S., Kamaruddin, S., Bharudin, I., Abu Bakar, F. D., & Abd. Murad, A. M. (2019). Identification and characterization of a mating signalling gene from an oil palm pathogen, Ganoderma boninense. In N. H. A. Rasol, K. Ibrahim, S. A. Hasbullah, M. H. H. Jumali, N. Ibrahim, M. M. Hanafiah, & M. T. Latif (Eds.), 2018 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2018 Postgraduate Colloquium [040005] (AIP Conference Proceedings; Vol. 2111). American Institute of Physics Inc.. https://doi.org/10.1063/1.5111244

Identification and characterization of a mating signalling gene from an oil palm pathogen, Ganoderma boninense. / Madihah, Ahmad Zairun; Idris, Abu Seman; Kamaruddin, Shazilah; Bharudin, Izwan; Abu Bakar, Farah Diba; Abd. Murad, Abdul Munir.

2018 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2018 Postgraduate Colloquium. ed. / Noor Hayati Ahmad Rasol; Kamarulzaman Ibrahim; Siti Aishah Hasbullah; Mohammad Hafizuddin Hj. Jumali; Nazlina Ibrahim; Marlia Mohd Hanafiah; Mohd Talib Latif. American Institute of Physics Inc., 2019. 040005 (AIP Conference Proceedings; Vol. 2111).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Madihah, AZ, Idris, AS, Kamaruddin, S, Bharudin, I, Abu Bakar, FD & Abd. Murad, AM 2019, Identification and characterization of a mating signalling gene from an oil palm pathogen, Ganoderma boninense. in NHA Rasol, K Ibrahim, SA Hasbullah, MHH Jumali, N Ibrahim, MM Hanafiah & MT Latif (eds), 2018 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2018 Postgraduate Colloquium., 040005, AIP Conference Proceedings, vol. 2111, American Institute of Physics Inc., 2018 UKM FST Postgraduate Colloquium, Selangor, Malaysia, 4/4/18. https://doi.org/10.1063/1.5111244
Madihah AZ, Idris AS, Kamaruddin S, Bharudin I, Abu Bakar FD, Abd. Murad AM. Identification and characterization of a mating signalling gene from an oil palm pathogen, Ganoderma boninense. In Rasol NHA, Ibrahim K, Hasbullah SA, Jumali MHH, Ibrahim N, Hanafiah MM, Latif MT, editors, 2018 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2018 Postgraduate Colloquium. American Institute of Physics Inc. 2019. 040005. (AIP Conference Proceedings). https://doi.org/10.1063/1.5111244
Madihah, Ahmad Zairun ; Idris, Abu Seman ; Kamaruddin, Shazilah ; Bharudin, Izwan ; Abu Bakar, Farah Diba ; Abd. Murad, Abdul Munir. / Identification and characterization of a mating signalling gene from an oil palm pathogen, Ganoderma boninense. 2018 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2018 Postgraduate Colloquium. editor / Noor Hayati Ahmad Rasol ; Kamarulzaman Ibrahim ; Siti Aishah Hasbullah ; Mohammad Hafizuddin Hj. Jumali ; Nazlina Ibrahim ; Marlia Mohd Hanafiah ; Mohd Talib Latif. American Institute of Physics Inc., 2019. (AIP Conference Proceedings).
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