Pengenalpastian dan pencirian gen trichoderma virens ukm1 mengekod enzim terlibat dalam pencuraian kitin krustasea

Translated title of the contribution: Identification and Characterisation of Trichoderma virens UKM1 Genes Encoding for Enzymes Involved in the Degradation of Crustacean Chitin

Abdul Munir Abd. Murad, Rafidah Badrun, Sakina Shahabudin, Shazilah Kamaruddin, Madihah Ahmad Zairun, Farahayu Khairuddin, Nor Muhammad Mahadi, Rosli Md Illias, Zamri Zainal, Farah Diba Abu Bakar

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Chitin is a structural polysaccharide which can be degraded by chitinolytic enzymes to various derivatives that can be utilised in medicine, agriculture and water treatment. The identification and characterisation of Trichoderma virens UKM1 genes encoding for enzymes involved in crustacean chitin degradation were carried out by generating expressed sequence tags (ESTs) and analysing gene expression via DNA microarray. Three cDNA libraries of T. virens UKM1 induced with chitin, glucosamine and chitosan, respectively, were constructed. A total of 1536 cDNA clones were sequenced and 1033 of high-quality ESTs were generated. Subsequently, differences in gene expression between cells grown in the presence and absence of crustacean chitin on the third and fifth days were determined. A total of 1824 cDNA clones were spotted on glass slides and co-hybridised with Cy3-or Cy5-labeled cDNA, synthesised from mRNA isolated from cells grown in medium containing crustacean chitin or glucose (control). A total of 91 and 61 genes were expressed by more than two-fold on the third and fifth day, respectively, when the fungus used crustacean chitin as the carbon source. Several genes encoding for chitinase such as ech1 and cht3 (endochitinases), nag1 (exochitinase) and nagB (glucosamine 6-P-deaminase) were found highly expressed on both days. In addition, genes encoding for hydrophobin, serine protease and several hypothetical proteins were also expressed at high levels when cells were exposed to crustacean chitin. These proteins may play significant role in the degradation of crustacean chitin.

Original languageUndefined/Unknown
Pages (from-to)715-724
Number of pages10
JournalSains Malaysiana
Volume42
Issue number6
Publication statusPublished - Jun 2013

Fingerprint

Trichoderma virens
chitin
Crustacea
degradation
enzymes
genes
glucosamine
expressed sequence tags
clones
gene expression
serine proteinases
cells
chitinase
water treatment
chitosan
cDNA libraries
glass
medicine
polysaccharides
proteins

Keywords

  • Chitin
  • Chitinase
  • DNA microarray
  • Expressed sequence tag
  • Trichoderma virens

ASJC Scopus subject areas

  • General

Cite this

Pengenalpastian dan pencirian gen trichoderma virens ukm1 mengekod enzim terlibat dalam pencuraian kitin krustasea. / Abd. Murad, Abdul Munir; Badrun, Rafidah; Shahabudin, Sakina; Kamaruddin, Shazilah; Zairun, Madihah Ahmad; Khairuddin, Farahayu; Mahadi, Nor Muhammad; Illias, Rosli Md; Zainal, Zamri; Abu Bakar, Farah Diba.

In: Sains Malaysiana, Vol. 42, No. 6, 06.2013, p. 715-724.

Research output: Contribution to journalArticle

Abd. Murad, AM, Badrun, R, Shahabudin, S, Kamaruddin, S, Zairun, MA, Khairuddin, F, Mahadi, NM, Illias, RM, Zainal, Z & Abu Bakar, FD 2013, 'Pengenalpastian dan pencirian gen trichoderma virens ukm1 mengekod enzim terlibat dalam pencuraian kitin krustasea', Sains Malaysiana, vol. 42, no. 6, pp. 715-724.
Abd. Murad AM, Badrun R, Shahabudin S, Kamaruddin S, Zairun MA, Khairuddin F et al. Pengenalpastian dan pencirian gen trichoderma virens ukm1 mengekod enzim terlibat dalam pencuraian kitin krustasea. Sains Malaysiana. 2013 Jun;42(6):715-724.
Abd. Murad, Abdul Munir ; Badrun, Rafidah ; Shahabudin, Sakina ; Kamaruddin, Shazilah ; Zairun, Madihah Ahmad ; Khairuddin, Farahayu ; Mahadi, Nor Muhammad ; Illias, Rosli Md ; Zainal, Zamri ; Abu Bakar, Farah Diba. / Pengenalpastian dan pencirian gen trichoderma virens ukm1 mengekod enzim terlibat dalam pencuraian kitin krustasea. In: Sains Malaysiana. 2013 ; Vol. 42, No. 6. pp. 715-724.
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abstract = "Chitin is a structural polysaccharide which can be degraded by chitinolytic enzymes to various derivatives that can be utilised in medicine, agriculture and water treatment. The identification and characterisation of Trichoderma virens UKM1 genes encoding for enzymes involved in crustacean chitin degradation were carried out by generating expressed sequence tags (ESTs) and analysing gene expression via DNA microarray. Three cDNA libraries of T. virens UKM1 induced with chitin, glucosamine and chitosan, respectively, were constructed. A total of 1536 cDNA clones were sequenced and 1033 of high-quality ESTs were generated. Subsequently, differences in gene expression between cells grown in the presence and absence of crustacean chitin on the third and fifth days were determined. A total of 1824 cDNA clones were spotted on glass slides and co-hybridised with Cy3-or Cy5-labeled cDNA, synthesised from mRNA isolated from cells grown in medium containing crustacean chitin or glucose (control). A total of 91 and 61 genes were expressed by more than two-fold on the third and fifth day, respectively, when the fungus used crustacean chitin as the carbon source. Several genes encoding for chitinase such as ech1 and cht3 (endochitinases), nag1 (exochitinase) and nagB (glucosamine 6-P-deaminase) were found highly expressed on both days. In addition, genes encoding for hydrophobin, serine protease and several hypothetical proteins were also expressed at high levels when cells were exposed to crustacean chitin. These proteins may play significant role in the degradation of crustacean chitin.",
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