Hypolipidemic activities of xanthorrhizol purified from centrifugal TLC

Seok Fang Oon, Meenakshii Nallappan, Nur Kartinee Kassim, Shamarina Shohaimi, Mohd Shazrul Fazry Sa`Ariwijaya, Thiam Tsui Tee, Yew Hoong Cheah

Research output: Contribution to journalArticle

Abstract

Hyperlipidemia is defined as the presence of either hypertriglyceridemia or hypercholesterolemia, which could cause atherosclerosis. Although hyperlipidemia can be treated by hypolipidemic drugs, they are limited due to lack of effectiveness and safety. Previous studies demonstrated that xanthorrhizol (XNT) isolated from Curcuma xanthorrhizza Roxb. reduced the levels of free fatty acid and triglyceride in vivo. However, its ability to inhibit cholesterol uptake in HT29 colon cells and adipogenesis in 3T3-L1 cells are yet to be reported. In this study, XNT purified from centrifugal TLC demonstrated 98.3% purity, indicating it could be an alternative purification method. The IC50 values of XNT were 30.81 ± 0.78 μg/mL in HT29 cells and 35.07 ± 0.24 μg/mL in 3T3-L1 adipocytes, respectively. Cholesterol uptake inhibition study using HT29 colon cells showed that XNT (15 μg/mL) significantly inhibited the fluorescent cholesterol analogue NBD uptake by up to 27 ± 3.1% relative to control. On the other hand, higher concentration of XNT (50 μg/mL) significantly suppressed the growth of 3T3-L1 adipocytes (5.9 ± 0.58%) compared to 3T3-L1 preadipocytes (81.31 ± 0.55%). XNT was found to impede adipogenesis of 3T3-L1 adipocytes in a dose-dependent manner from 3.125 to 12.5 μg/mL, where 12.5 μg/mL significantly suppressed 36.13 ± 2.1% of lipid accumulation. We postulate that inhibition of cholesterol uptake, adipogenesis, preadipocyte and adipocyte number may be utilized as treatment modalities to reduce the prevalence of lipidemia. To conclude, XNT could be a potential hypolipidemic agent to improve cardiovascular health in the future.

Original languageEnglish
Pages (from-to)1403-1408
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume478
Issue number3
DOIs
Publication statusPublished - 2016

Fingerprint

Adipocytes
Adipogenesis
HT29 Cells
Hyperlipidemias
Cholesterol
Hypolipidemic Agents
Colon
3T3-L1 Cells
Curcuma
Hypertriglyceridemia
xanthorrhizol
Hypercholesterolemia
Nonesterified Fatty Acids
Inhibitory Concentration 50
Purification
Atherosclerosis
Triglycerides
Health
Lipids
Safety

Keywords

  • 3T3-L1 adipocytes
  • Adipogenesis
  • Centrifugal TLC
  • Cholesterol
  • HT29 colon cells
  • Xanthorrhizol

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Hypolipidemic activities of xanthorrhizol purified from centrifugal TLC. / Oon, Seok Fang; Nallappan, Meenakshii; Kassim, Nur Kartinee; Shohaimi, Shamarina; Sa`Ariwijaya, Mohd Shazrul Fazry; Tee, Thiam Tsui; Cheah, Yew Hoong.

In: Biochemical and Biophysical Research Communications, Vol. 478, No. 3, 2016, p. 1403-1408.

Research output: Contribution to journalArticle

Oon, Seok Fang ; Nallappan, Meenakshii ; Kassim, Nur Kartinee ; Shohaimi, Shamarina ; Sa`Ariwijaya, Mohd Shazrul Fazry ; Tee, Thiam Tsui ; Cheah, Yew Hoong. / Hypolipidemic activities of xanthorrhizol purified from centrifugal TLC. In: Biochemical and Biophysical Research Communications. 2016 ; Vol. 478, No. 3. pp. 1403-1408.
@article{240c5102154b4659bceeb6cc5a4dca9d,
title = "Hypolipidemic activities of xanthorrhizol purified from centrifugal TLC",
abstract = "Hyperlipidemia is defined as the presence of either hypertriglyceridemia or hypercholesterolemia, which could cause atherosclerosis. Although hyperlipidemia can be treated by hypolipidemic drugs, they are limited due to lack of effectiveness and safety. Previous studies demonstrated that xanthorrhizol (XNT) isolated from Curcuma xanthorrhizza Roxb. reduced the levels of free fatty acid and triglyceride in vivo. However, its ability to inhibit cholesterol uptake in HT29 colon cells and adipogenesis in 3T3-L1 cells are yet to be reported. In this study, XNT purified from centrifugal TLC demonstrated 98.3{\%} purity, indicating it could be an alternative purification method. The IC50 values of XNT were 30.81 ± 0.78 μg/mL in HT29 cells and 35.07 ± 0.24 μg/mL in 3T3-L1 adipocytes, respectively. Cholesterol uptake inhibition study using HT29 colon cells showed that XNT (15 μg/mL) significantly inhibited the fluorescent cholesterol analogue NBD uptake by up to 27 ± 3.1{\%} relative to control. On the other hand, higher concentration of XNT (50 μg/mL) significantly suppressed the growth of 3T3-L1 adipocytes (5.9 ± 0.58{\%}) compared to 3T3-L1 preadipocytes (81.31 ± 0.55{\%}). XNT was found to impede adipogenesis of 3T3-L1 adipocytes in a dose-dependent manner from 3.125 to 12.5 μg/mL, where 12.5 μg/mL significantly suppressed 36.13 ± 2.1{\%} of lipid accumulation. We postulate that inhibition of cholesterol uptake, adipogenesis, preadipocyte and adipocyte number may be utilized as treatment modalities to reduce the prevalence of lipidemia. To conclude, XNT could be a potential hypolipidemic agent to improve cardiovascular health in the future.",
keywords = "3T3-L1 adipocytes, Adipogenesis, Centrifugal TLC, Cholesterol, HT29 colon cells, Xanthorrhizol",
author = "Oon, {Seok Fang} and Meenakshii Nallappan and Kassim, {Nur Kartinee} and Shamarina Shohaimi and Sa`Ariwijaya, {Mohd Shazrul Fazry} and Tee, {Thiam Tsui} and Cheah, {Yew Hoong}",
year = "2016",
doi = "10.1016/j.bbrc.2016.08.136",
language = "English",
volume = "478",
pages = "1403--1408",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Hypolipidemic activities of xanthorrhizol purified from centrifugal TLC

AU - Oon, Seok Fang

AU - Nallappan, Meenakshii

AU - Kassim, Nur Kartinee

AU - Shohaimi, Shamarina

AU - Sa`Ariwijaya, Mohd Shazrul Fazry

AU - Tee, Thiam Tsui

AU - Cheah, Yew Hoong

PY - 2016

Y1 - 2016

N2 - Hyperlipidemia is defined as the presence of either hypertriglyceridemia or hypercholesterolemia, which could cause atherosclerosis. Although hyperlipidemia can be treated by hypolipidemic drugs, they are limited due to lack of effectiveness and safety. Previous studies demonstrated that xanthorrhizol (XNT) isolated from Curcuma xanthorrhizza Roxb. reduced the levels of free fatty acid and triglyceride in vivo. However, its ability to inhibit cholesterol uptake in HT29 colon cells and adipogenesis in 3T3-L1 cells are yet to be reported. In this study, XNT purified from centrifugal TLC demonstrated 98.3% purity, indicating it could be an alternative purification method. The IC50 values of XNT were 30.81 ± 0.78 μg/mL in HT29 cells and 35.07 ± 0.24 μg/mL in 3T3-L1 adipocytes, respectively. Cholesterol uptake inhibition study using HT29 colon cells showed that XNT (15 μg/mL) significantly inhibited the fluorescent cholesterol analogue NBD uptake by up to 27 ± 3.1% relative to control. On the other hand, higher concentration of XNT (50 μg/mL) significantly suppressed the growth of 3T3-L1 adipocytes (5.9 ± 0.58%) compared to 3T3-L1 preadipocytes (81.31 ± 0.55%). XNT was found to impede adipogenesis of 3T3-L1 adipocytes in a dose-dependent manner from 3.125 to 12.5 μg/mL, where 12.5 μg/mL significantly suppressed 36.13 ± 2.1% of lipid accumulation. We postulate that inhibition of cholesterol uptake, adipogenesis, preadipocyte and adipocyte number may be utilized as treatment modalities to reduce the prevalence of lipidemia. To conclude, XNT could be a potential hypolipidemic agent to improve cardiovascular health in the future.

AB - Hyperlipidemia is defined as the presence of either hypertriglyceridemia or hypercholesterolemia, which could cause atherosclerosis. Although hyperlipidemia can be treated by hypolipidemic drugs, they are limited due to lack of effectiveness and safety. Previous studies demonstrated that xanthorrhizol (XNT) isolated from Curcuma xanthorrhizza Roxb. reduced the levels of free fatty acid and triglyceride in vivo. However, its ability to inhibit cholesterol uptake in HT29 colon cells and adipogenesis in 3T3-L1 cells are yet to be reported. In this study, XNT purified from centrifugal TLC demonstrated 98.3% purity, indicating it could be an alternative purification method. The IC50 values of XNT were 30.81 ± 0.78 μg/mL in HT29 cells and 35.07 ± 0.24 μg/mL in 3T3-L1 adipocytes, respectively. Cholesterol uptake inhibition study using HT29 colon cells showed that XNT (15 μg/mL) significantly inhibited the fluorescent cholesterol analogue NBD uptake by up to 27 ± 3.1% relative to control. On the other hand, higher concentration of XNT (50 μg/mL) significantly suppressed the growth of 3T3-L1 adipocytes (5.9 ± 0.58%) compared to 3T3-L1 preadipocytes (81.31 ± 0.55%). XNT was found to impede adipogenesis of 3T3-L1 adipocytes in a dose-dependent manner from 3.125 to 12.5 μg/mL, where 12.5 μg/mL significantly suppressed 36.13 ± 2.1% of lipid accumulation. We postulate that inhibition of cholesterol uptake, adipogenesis, preadipocyte and adipocyte number may be utilized as treatment modalities to reduce the prevalence of lipidemia. To conclude, XNT could be a potential hypolipidemic agent to improve cardiovascular health in the future.

KW - 3T3-L1 adipocytes

KW - Adipogenesis

KW - Centrifugal TLC

KW - Cholesterol

KW - HT29 colon cells

KW - Xanthorrhizol

UR - http://www.scopus.com/inward/record.url?scp=84994005739&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84994005739&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2016.08.136

DO - 10.1016/j.bbrc.2016.08.136

M3 - Article

C2 - 27576204

AN - SCOPUS:84994005739

VL - 478

SP - 1403

EP - 1408

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -