Hydrogen production performance by Enterobacter cloacae KBH3 isolated from termite guts

Irina Harun, Jamaliah Md Jahim, Nurina Anuar, Osman Hassan

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Two out of six bacterial isolates obtained from the guts of Globitermes sp. termites were identified as hydrogen-producing bacteria. One isolate, Enterobacter cloacae KBH3, was characterised using the BIOLOG identification system and 16S rRNA gene analysis. In a batch fermentation study to evaluate its growth in defined medium, E. cloacae KBH3 produced 154 ml H2 per litre medium with approximately 50% hydrogen content. The carbon utilisation results suggest that E. cloacae KBH3 have the potential to be a good hydrogen producer. This strain is also able to produce hydrogen within a wide range of temperatures (28-40 °C) and pH (4.5-8). In several fermentation runs, the pH of the culture dropped from 6.5 to 5.36 within the first 3 h, which was mostly due to the biosynthesis of formate. An increase of cumulative hydrogen production was recorded as well as a decrease in the concentration of formate, indicating the importance of the formate pathway for hydrogen production. The highest rate of hydrogen production of 180.74 ml H2/l/h was achieved when lactate and acetate were at their highest concentrations. Most of the hydrogen gas was produced during the exponential growth phase, and the biogas continued to be produced during the stationary phase. The specific growth rate was calculated to be 0.224 per hour while the hydrogen yield was 1.8 mol of hydrogen per mol of glucose. At the end of the batch study, the highest cumulative hydrogen production was 2404 ml H2 per litre of fermentation medium.

Original languageEnglish
Pages (from-to)15052-15061
Number of pages10
JournalInternational Journal of Hydrogen Energy
Volume37
Issue number20
DOIs
Publication statusPublished - Oct 2012

Fingerprint

hydrogen production
Hydrogen production
Hydrogen
hydrogen
fermentation
formates
Fermentation
lactates
biosynthesis
system identification
Biosynthesis
Biogas
glucose
genes
bacteria
Glucose
acetates
Identification (control systems)
Bacteria
Genes

Keywords

  • Bacterial characterization
  • Biohydrogen
  • Dark fermentation
  • Enterobacter cloacae
  • Termite gut bacteria

ASJC Scopus subject areas

  • Renewable Energy, Sustainability and the Environment
  • Fuel Technology
  • Condensed Matter Physics
  • Energy Engineering and Power Technology

Cite this

Hydrogen production performance by Enterobacter cloacae KBH3 isolated from termite guts. / Harun, Irina; Md Jahim, Jamaliah; Anuar, Nurina; Hassan, Osman.

In: International Journal of Hydrogen Energy, Vol. 37, No. 20, 10.2012, p. 15052-15061.

Research output: Contribution to journalArticle

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