Hydrogen peroxide stimulates glucose uptake in myotube cells via the insulin signaling pathway

Musalmah Mazlan, Rehanna Mansor, Wan Zurinah Ngah

    Research output: Contribution to journalArticle

    2 Citations (Scopus)

    Abstract

    Recent studies suggest that reactive oxygen species (ROS) such as hydrogen peroxide (H2O2) may exert beneficial effects on health. Therefore this study evaluated the effects of H2O2 and gamma-tocotrienol (GTT), an antioxidant, on D-[2-3H] Glucose uptake by myotubes compared to insulin. Results showed that H2O2 and insulin significantly increased D-[2-3H] Glucose uptake. This was associated with an increased in Vmax but Michaelis constant (Km) of the transport system remained unchanged indicating that an increase in amount, rather than affinity, of the glucose transporter was involved in the process. Western blot studies confirmed that H2O2 increased the expressions of insulin sensitive glucose transporter, GLUT4 and also hexokinase (an enzyme which phosphorylates glucose and indirectly stimulates glucose uptake by cells). H2O2 and insulin also stimulated increased in PPAR-γ and IRS-I, which are proteins of the insulin transduction pathway. The present data however showed that GTT did not exert any effect on glucose uptake. Thus, the study showed that H2O2 mimicked insulin action in stimulating glucose uptake in myotube cells by influencing the insulin signaling pathway.

    Original languageEnglish
    Pages (from-to)315-320
    Number of pages6
    JournalSains Malaysiana
    Volume39
    Issue number2
    Publication statusPublished - Apr 2010

    Fingerprint

    Skeletal Muscle Fibers
    Hydrogen Peroxide
    Insulin
    Glucose
    Facilitative Glucose Transport Proteins
    Peroxisome Proliferator-Activated Receptors
    Hexokinase
    Reactive Oxygen Species
    Antioxidants
    Western Blotting
    Health
    Enzymes
    Proteins

    Keywords

    • Gamma-tocotrienol
    • Glucose uptake
    • Hydrogen peroxide
    • Insulin action
    • Myotube cells

    ASJC Scopus subject areas

    • General

    Cite this

    Hydrogen peroxide stimulates glucose uptake in myotube cells via the insulin signaling pathway. / Mazlan, Musalmah; Mansor, Rehanna; Ngah, Wan Zurinah.

    In: Sains Malaysiana, Vol. 39, No. 2, 04.2010, p. 315-320.

    Research output: Contribution to journalArticle

    Mazlan, Musalmah ; Mansor, Rehanna ; Ngah, Wan Zurinah. / Hydrogen peroxide stimulates glucose uptake in myotube cells via the insulin signaling pathway. In: Sains Malaysiana. 2010 ; Vol. 39, No. 2. pp. 315-320.
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