Human serum promotes the proliferation but not the sternness genes expression of human adipose-derived stem cells

Chua Kien Hui, Wan Kamarul Zaman Wan Safwani, Seah Shiao Chin, Annisaa Abu Samah Abdul Malek, Noormazita Hassan, Muhamad Syakeer Fazil, Raja Abdul Wafy Raja Muhammad Rooshdi, Adila A. Hamid, Somasundaram Sathappan

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Recently human adipose-derived stem cells (ASCs) have shown much therapeutic potential in regenerative medicine. However, fetal bovine serum (FBS) used in culturing human cells may give risk to viral and prion transmission as well as immune rejection. Human serum (HS) is a safer growth supplement in human cell culture but its effects have not been well established. Therefore the objectives of this study were to compare the effects of HS versus FBS on the proliferation and sternness gene expression of ASCs. ASCs were cultured for 5 passages in medium supplemented with either 10% HS or 10% FBS. ASCs proliferation rate and viability were determined at every passage. Total RNA was extracted at passage 5 (P5) and quantitative PCR was carried out to determine the sternness gene expression level of SOX-2, Nanog3, BST-1, REX-1, ABCG2 and FGF-4. The results showed ASC cultured in 10% HS scored greater proliferation rates and viability compared to 10% FBS. ASCs proliferated significantly faster in 10% HS compared to 10% FBS at P2, P3, and P4 (p < 0.05). In quantitative gene expression analysis, ASCs cultured in 10% FBS showed a significant increase of BST-1, REX-1 and ABCG2 expression compared to 10% HS. In conclusion, HS promotes ASCs proliferation and viability but its ability to support the sternness property of ASCs was inferior to FBS.

Original languageEnglish
Pages (from-to)1306-1315
Number of pages10
JournalBiotechnology and Bioprocess Engineering
Volume17
Issue number6
DOIs
Publication statusPublished - Nov 2012

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Stem cells
Gene expression
Stem Cells
Gene Expression
Serum
Cell proliferation
Prions
Cell Survival
RNA
Cell culture
Cell Proliferation
Fibroblast Growth Factor 1
Regenerative Medicine
Cells
Cell Culture Techniques

Keywords

  • Adipose
  • Cell proliferation
  • Gene expression
  • Human serum
  • Regenerative medicine
  • Stem cells

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Biomedical Engineering
  • Bioengineering

Cite this

Human serum promotes the proliferation but not the sternness genes expression of human adipose-derived stem cells. / Kien Hui, Chua; Safwani, Wan Kamarul Zaman Wan; Chin, Seah Shiao; Malek, Annisaa Abu Samah Abdul; Hassan, Noormazita; Fazil, Muhamad Syakeer; Rooshdi, Raja Abdul Wafy Raja Muhammad; A. Hamid, Adila; Sathappan, Somasundaram.

In: Biotechnology and Bioprocess Engineering, Vol. 17, No. 6, 11.2012, p. 1306-1315.

Research output: Contribution to journalArticle

Kien Hui, Chua ; Safwani, Wan Kamarul Zaman Wan ; Chin, Seah Shiao ; Malek, Annisaa Abu Samah Abdul ; Hassan, Noormazita ; Fazil, Muhamad Syakeer ; Rooshdi, Raja Abdul Wafy Raja Muhammad ; A. Hamid, Adila ; Sathappan, Somasundaram. / Human serum promotes the proliferation but not the sternness genes expression of human adipose-derived stem cells. In: Biotechnology and Bioprocess Engineering. 2012 ; Vol. 17, No. 6. pp. 1306-1315.
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abstract = "Recently human adipose-derived stem cells (ASCs) have shown much therapeutic potential in regenerative medicine. However, fetal bovine serum (FBS) used in culturing human cells may give risk to viral and prion transmission as well as immune rejection. Human serum (HS) is a safer growth supplement in human cell culture but its effects have not been well established. Therefore the objectives of this study were to compare the effects of HS versus FBS on the proliferation and sternness gene expression of ASCs. ASCs were cultured for 5 passages in medium supplemented with either 10{\%} HS or 10{\%} FBS. ASCs proliferation rate and viability were determined at every passage. Total RNA was extracted at passage 5 (P5) and quantitative PCR was carried out to determine the sternness gene expression level of SOX-2, Nanog3, BST-1, REX-1, ABCG2 and FGF-4. The results showed ASC cultured in 10{\%} HS scored greater proliferation rates and viability compared to 10{\%} FBS. ASCs proliferated significantly faster in 10{\%} HS compared to 10{\%} FBS at P2, P3, and P4 (p < 0.05). In quantitative gene expression analysis, ASCs cultured in 10{\%} FBS showed a significant increase of BST-1, REX-1 and ABCG2 expression compared to 10{\%} HS. In conclusion, HS promotes ASCs proliferation and viability but its ability to support the sternness property of ASCs was inferior to FBS.",
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AU - Kien Hui, Chua

AU - Safwani, Wan Kamarul Zaman Wan

AU - Chin, Seah Shiao

AU - Malek, Annisaa Abu Samah Abdul

AU - Hassan, Noormazita

AU - Fazil, Muhamad Syakeer

AU - Rooshdi, Raja Abdul Wafy Raja Muhammad

AU - A. Hamid, Adila

AU - Sathappan, Somasundaram

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AB - Recently human adipose-derived stem cells (ASCs) have shown much therapeutic potential in regenerative medicine. However, fetal bovine serum (FBS) used in culturing human cells may give risk to viral and prion transmission as well as immune rejection. Human serum (HS) is a safer growth supplement in human cell culture but its effects have not been well established. Therefore the objectives of this study were to compare the effects of HS versus FBS on the proliferation and sternness gene expression of ASCs. ASCs were cultured for 5 passages in medium supplemented with either 10% HS or 10% FBS. ASCs proliferation rate and viability were determined at every passage. Total RNA was extracted at passage 5 (P5) and quantitative PCR was carried out to determine the sternness gene expression level of SOX-2, Nanog3, BST-1, REX-1, ABCG2 and FGF-4. The results showed ASC cultured in 10% HS scored greater proliferation rates and viability compared to 10% FBS. ASCs proliferated significantly faster in 10% HS compared to 10% FBS at P2, P3, and P4 (p < 0.05). In quantitative gene expression analysis, ASCs cultured in 10% FBS showed a significant increase of BST-1, REX-1 and ABCG2 expression compared to 10% HS. In conclusion, HS promotes ASCs proliferation and viability but its ability to support the sternness property of ASCs was inferior to FBS.

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