Human Serum Is an Advantageous Supplement for Human Dermal Fibroblast Expansion: Clinical Implications for Tissue Engineering of Skin

Mazlyzam Abdul Latif, Bin Saim Aminuddin, Lokman Saim, Ruszymah Idrus

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Background: Standard fibroblast culture medium usually contains fetal bovine serum (FBS). In theory, unknown risks of infection from bovine disease or immune reaction to foreign proteins may occur if standard culture method is used for future human tissue-engineering development. Human serum (HS) theoretically would be another choice in providing a safer approach and autologous clinically reliable cells. Methods: Isolated human dermal fibroblasts were culture-expanded in an equal volume mixture of Ham's F12 medium and Dulbecco's Modified Eagle Medium (DMEM) supplemented with either 10% HS or 10% FBS from passage 0 to passage 3. Effects of 10% HS and 10% FBS on human fibroblast viability, growth kinetics, cell cycle analysis and gene expressions were investigated and compared. Results: Generally, fibroblast viability cultured in HS supplementations was much higher compared to FBS supplementation. Fibroblast proliferations were faster in HS supplementations with shorter doubling time. Cell cycle analysis showed fibroblasts cultured with HS supplementations have higher S-phase ratio compared to FBS. Gene expression levels by quantitative reverse transcriptase-polymerose chain reaction (RT-PCR) showed cultured fibroblasts with HS supplementation maintains expression of collagen type I collagen, increased expression of type III collagen and fibronectin and reduced expression of α-smooth muscle actin (α-SMA) compared to FBS. Conclusions: Results demonstrated potential advantages of HS vs. FBS in generating larger numbers of cultured dermal fibroblasts in a shorter period of time. HS also influenced mRNA expression of type III collagen and fibronectin (upregulated) and α-SMA (downregulated), which are important extracellular matrix proteins in wound healing.

Original languageEnglish
Pages (from-to)743-752
Number of pages10
JournalArchives of Medical Research
Volume39
Issue number8
DOIs
Publication statusPublished - Nov 2008

Fingerprint

Tissue Engineering
Fibroblasts
Skin
Serum
Collagen Type III
Fibronectins
Cattle Diseases
Gene Expression
cdc Genes
Eagles
Human Engineering
Extracellular Matrix Proteins
RNA-Directed DNA Polymerase
Collagen Type I
S Phase
Wound Healing
Smooth Muscle
Culture Media
Actins

Keywords

  • Cell culture
  • Cell cycle
  • Dermis
  • Fibroblast
  • Gene expression
  • Serum

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Human Serum Is an Advantageous Supplement for Human Dermal Fibroblast Expansion : Clinical Implications for Tissue Engineering of Skin. / Abdul Latif, Mazlyzam; Aminuddin, Bin Saim; Saim, Lokman; Idrus, Ruszymah.

In: Archives of Medical Research, Vol. 39, No. 8, 11.2008, p. 743-752.

Research output: Contribution to journalArticle

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