Human nasal turbinates as a viable source of respiratory epithelial cells using co-culture system versus dispase dissociation technique

Nur Adelina Ahmad Noruddin, Aminuddin B. Saim, Chua Kien Hui, Ruszymah Idrus

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

OBJECTIVE: To compare a co-culture system with a conventional dispase-dissociation method for obtaining functional human respiratory epithelial cells from the nasal turbinates for tissue engineering application. METHODS: Human respiratory epithelial cells were serially passaged using a co-culture system and a conventional dispase-dissociation technique. The growth kinetics and gene expression levels of the cultured respiratory epithelial cells were compared. Four genes were investigated, namely cytokeratin-18, a marker for ciliated and secretory epithelial cells; cytokeratin-14, a marker for basal epithelial cells; MKI67, a proliferation marker; and MUC5B, a marker for mucin secretion. Immunocytochemical analysis was performed using monoclonal antibodies against the high molecular-weight cytokeratin 34βE12, cytokeratin 18, and MUC5A to investigate the protein expression from cultured respiratory epithelial cells. RESULTS: Respiratory epithelial cells cultured using both methods maintained polygonal morphology throughout the passages. At passage 1, co-cultured respiratory epithelial showed a 2.6-times higher growth rate compared to conventional dispase dissociation technique, and 7.8 times higher at passage 2. Better basal gene expression was observed by co-cultured respiratory epithelial cells compared to dispase dissociated cells. Immunocytochemical analyses were positive for the respiratory epithelial cells cultured using both techniques. CONCLUSION: Co-culture system produced superior quality of cultured human respiratory epithelial cells from the nasal turbinates as compared to dispase dissociation technique.

Original languageEnglish
Pages (from-to)2139-2145
Number of pages7
JournalLaryngoscope
Volume117
Issue number12
DOIs
Publication statusPublished - Dec 2007

Fingerprint

Turbinates
Coculture Techniques
Nose
Epithelial Cells
Keratin-18
dispase
Keratin-14
Gene Expression
Mucins
Tissue Engineering
Growth
Keratins
Molecular Weight
Monoclonal Antibodies

Keywords

  • Co-culture system
  • Gene expression analysis
  • Human respiratory epithelial cells
  • Tissue engineering

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Human nasal turbinates as a viable source of respiratory epithelial cells using co-culture system versus dispase dissociation technique. / Noruddin, Nur Adelina Ahmad; Saim, Aminuddin B.; Kien Hui, Chua; Idrus, Ruszymah.

In: Laryngoscope, Vol. 117, No. 12, 12.2007, p. 2139-2145.

Research output: Contribution to journalArticle

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