Human forniceal region is the stem cell-rich zone of the conjunctival epithelium

Mohd Hairul Nizam Harun, Siti Norzalehawati Sepian, Chua Kien Hui, Abd Rahman Ropilah, Norzana Abd. Ghafar, Jemaima Che Hamzah, Ruszymah Idrus, Faridah Hanom Annuar

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The anterior surface of the eye is covered by several physically contiguous but histologically distinguishable epithelia overlying the cornea, limbus, bulbar conjunctiva, fornix conjunctiva, and palpebral conjunctiva. The self-renewing nature of the conjunctival epithelia makes their long-term survival ultimately dependent on small populations of stem cells. Hence, the objective of this study was to investigate the expression of the stem cell genes Sox2, OCT4, NANOG, Rex1, NES, and ABCG2 in cultured human conjunctival epithelium from different conjunctival zones, namely, the bulbar, palpebral and fornix zones. Three samples were taken from patients with primary pterygium and cataract (age range 56-66 years) who presented to our eye clinic at the UKM Medical Centre. The eye was examined with slit lamp to ensure there was no underlying ocular surface diseases and glaucoma. Conjunctival tissue was taken from patients who underwent a standard cataract or pterygium operation as a primary procedure. Tissues were digested, cultured, and propagated until an adequate number of cells was obtained. Total RNA was extracted and subjected to expression analysis of conjunctival epithelium genes (KRT4, KRT13, KRT19) and stem cell genes (Sox2, OCT4, NANOG, Rex1, NES, ABCG2) by reverse transcriptase-PCR and 2% agarose gel electrophoresis. The expression of Sox2, OCT4, and NANOG genes were detected in the fornical cells, while bulbar cells only expressed Sox2 and palpebral cells only expressed OCT4. Based on these results, the human forniceal region expresses a higher number of stem cell genes than the palpebral and bulbar conjunctiva.

Original languageEnglish
Pages (from-to)35-40
Number of pages6
JournalHuman Cell
Volume26
Issue number1
DOIs
Publication statusPublished - Mar 2013

Fingerprint

Conjunctiva
Stem Cells
Epithelium
Pterygium
Genes
Eyelids
Cataract
Limbus Corneae
Eye Diseases
Agar Gel Electrophoresis
Reverse Transcriptase Polymerase Chain Reaction
Glaucoma
Cell Count
RNA
Survival
Population

Keywords

  • Bulbar
  • Conjunctiva
  • Fornix
  • Palpebral
  • Stem cell
  • Tissue engineering

ASJC Scopus subject areas

  • Cell Biology
  • Cancer Research

Cite this

Human forniceal region is the stem cell-rich zone of the conjunctival epithelium. / Harun, Mohd Hairul Nizam; Sepian, Siti Norzalehawati; Kien Hui, Chua; Ropilah, Abd Rahman; Abd. Ghafar, Norzana; Che Hamzah, Jemaima; Idrus, Ruszymah; Annuar, Faridah Hanom.

In: Human Cell, Vol. 26, No. 1, 03.2013, p. 35-40.

Research output: Contribution to journalArticle

Harun, Mohd Hairul Nizam ; Sepian, Siti Norzalehawati ; Kien Hui, Chua ; Ropilah, Abd Rahman ; Abd. Ghafar, Norzana ; Che Hamzah, Jemaima ; Idrus, Ruszymah ; Annuar, Faridah Hanom. / Human forniceal region is the stem cell-rich zone of the conjunctival epithelium. In: Human Cell. 2013 ; Vol. 26, No. 1. pp. 35-40.
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