Human chorion-derived stem cells

Changes in stem cell properties during serial passage

Mohd Manzor Nur Fariha, Chua Kien Hui, Geok Chin Tan, Ay Eeng Tan, Abdul Rahman Hayati

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Background aims. Fetal membrane from human placenta tissue has been described as a potential source of stem cells. Despite abundant literature on amnion stem cells, there are limited studies on the stem cell properties of chorion-derived stem cells. Methods. The main aim was to determine the stemness properties of serial-passaged human chorion-derived stem cells (hCDSC). Quantitative polymerase chain reaction (PCR) was performed to reveal the following stemness gene expression in serial-passaged hCDSC: Oct-4, Sox-2, FGF-4, Rex-1, TERT, Nanog (3), Nestin, FZD-9, ABCG-2 and BST-1. Cell growth rate was evaluated from passage (P) 1 until P5. The colony-forming unit-fibroblast (CFU-F) frequency of P3 and P5 cells and multilineage differentiation potential of P5 cells were determined. The immunophenotype of hCDSC was compared using the surface markers CD9, CD31, CD34, CD44, CD45, CD73, CD90, CD117, HLA-ABC and HLA-DR, -DP and -DQ. Immunostaining for trophoblast markers was done on P0, P1, P3 and P5 cells to detect the contamination of trophoblasts in culture, while chromosomal abnormality was screened by cytogenetic analysis of P5 cells. Results. The surface markers for mesenchymal lineage in hCDSC were more highly expressed at P5 compared with P3 and P0, indicating the increased purity of these stem cells after serial passage. Indeed, all the stemness genes except TERT were expressed at P1, P3 and P5 hCDSC. Furthermore, human chorion contained high clonogenic precursors with a 1:30 CFU-F frequency. Successful adipogenic, chondrogenic and osteogenic differentiation demonstrated the multilineage potential of hCDSC. The karyotyping analysis showed hCDSC maintained chromosomal stability after serial passage. Conclusions. hCDSC retain multipotent potential even at later passages, hence are a promising source for cell therapy in the future.

Original languageEnglish
Pages (from-to)582-593
Number of pages12
JournalCytotherapy
Volume13
Issue number5
DOIs
Publication statusPublished - May 2011

Fingerprint

Serial Passage
Chorion
Stem Cells
Trophoblasts
Fibroblasts
HLA-DP Antigens
Multipotent Stem Cells
Extraembryonic Membranes
Nestin
Karyotyping
Chromosomal Instability
Amnion
Cytogenetic Analysis
HLA-DR Antigens
Fibroblast Growth Factor 2
Cell- and Tissue-Based Therapy
Chromosome Aberrations

Keywords

  • cell differentiation
  • fetal stem cells
  • gene expression
  • human placenta
  • immunophenotype
  • quantitative polymerase chain reaction
  • serial passage

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Molecular Medicine

Cite this

Human chorion-derived stem cells : Changes in stem cell properties during serial passage. / Nur Fariha, Mohd Manzor; Kien Hui, Chua; Tan, Geok Chin; Tan, Ay Eeng; Hayati, Abdul Rahman.

In: Cytotherapy, Vol. 13, No. 5, 05.2011, p. 582-593.

Research output: Contribution to journalArticle

Nur Fariha, Mohd Manzor ; Kien Hui, Chua ; Tan, Geok Chin ; Tan, Ay Eeng ; Hayati, Abdul Rahman. / Human chorion-derived stem cells : Changes in stem cell properties during serial passage. In: Cytotherapy. 2011 ; Vol. 13, No. 5. pp. 582-593.
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AU - Hayati, Abdul Rahman

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