Human Amniotic Membrane with Aligned Electrospun Fiber as Scaffold for Aligned Tissue Regeneration

Hanis Hasmad, Mohd Reusmaazran Yusof, Zainul Rashid Mohd. Razi, Ruszymah Idrus, Shiplu Roy Chowdhury

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Fabrication of composite scaffolds is one of the strategies proposed to enhance the functionality of tissue-engineered scaffolds for improved tissue regeneration. By combining multiple elements together, unique biomimetic scaffolds with desirable physical and mechanical properties can be tailored for tissue-specific applications. Despite having a highly porous structure, the utility of electrospun fibers (EF) as scaffold is usually hampered by their insufficient mechanical strength. In this study, we attempted to produce a mechanically competent scaffold with cell-guiding ability by fabricating aligned poly lactic-co-glycolic acid (PLGA) fibers on decellularized human amniotic membrane (HAM), known to possess favorable tensile and wound healing properties. Decellularization of HAM in 18.75 μg/mL of thermolysin followed by a brief treatment in 0.25 M sodium hydroxide efficiently removed the amniotic epithelium and preserved the ultrastructure of the underlying extracellular matrix. The electrospinning of 20% (w/v) PLGA 50:50 polymer on HAM yielded beadless fibers with straight morphology. Subsequent physical characterization revealed that EF-HAM scaffold with a 3-min fabrication had the most aligned fibers with the lowest fiber diameter in comparison with EF-HAM 5- and 7-min scaffolds. Hydrated EF-HAM scaffolds with 3-min deposition had a greater tensile strength than the other scaffolds despite having thinner fibers. Nevertheless, wet HAM and EF-HAMs regardless of the fiber thicknesses had a significantly lower Young's modulus, and hence, a higher elasticity compared with dry HAM and EF-HAMs. Biocompatibility analysis showed that the viability and migration rate of skeletal muscle cells on EF-HAMs were similar to control and HAM alone. Skeletal muscle cells seeded on HAM were shown to display random orientation, whereas cells on EF-HAM scaffolds were oriented along the alignment of the electrospun PLGA fibers. In summary, besides having good mechanical strength and elasticity, EF-HAM scaffold design decorated with aligned fiber topography holds a promising potential for use in the development of aligned tissue constructs.

Original languageEnglish
Pages (from-to)368-378
Number of pages11
JournalTissue Engineering - Part C: Methods
Volume24
Issue number6
DOIs
Publication statusPublished - 1 Jun 2018
Externally publishedYes

Fingerprint

Tissue Scaffolds
Tissue regeneration
Amnion
Scaffolds
Regeneration
Membranes
Fibers
Elasticity
Muscle Cells
Skeletal Muscle
Thermolysin
Strength of materials
Sodium Hydroxide
Biomimetics
Muscle
Acids
Tensile Strength
Elastic Modulus
Cells
Tissue

Keywords

  • aligned fiber
  • biomaterial
  • electrospinning
  • human amniotic membrane
  • skeletal muscle
  • tissue decellularization

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering

Cite this

Human Amniotic Membrane with Aligned Electrospun Fiber as Scaffold for Aligned Tissue Regeneration. / Hasmad, Hanis; Yusof, Mohd Reusmaazran; Mohd. Razi, Zainul Rashid; Idrus, Ruszymah; Chowdhury, Shiplu Roy.

In: Tissue Engineering - Part C: Methods, Vol. 24, No. 6, 01.06.2018, p. 368-378.

Research output: Contribution to journalArticle

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