Human adipose tissue derived stem cells as a source of smooth muscle cells in the regeneration of muscular layer of urinary bladder wall

Salah Abood Salem, Angela Ng Min Hwei, Chee Kong Christopher Ho, Ismail Sagap, Ruszymah Idrus, Zulkifili M.D. Zainuddin

Research output: Contribution to journalArticle

Abstract

Adipose tissue provides an abundant source of multipotent cells for cell-based regeneration strategies for urinary bladder smooth muscle repair. Our objective is to confirm that adipose derived stem cells (HADSCs) can be differentiated to smooth muscle cells. In this study adipose tissue sample was digested with 0.075% collagenase, and the resulting HADSCs cultured and expanded in vitro. HADSCs at passage 2 was differentiated by incubation in smooth muscle inductive media (SMIM) consisting of MCDB 131 medium, 1% FBS and 100 U/ml heparin. ADSCs in non-inductive media were used as control. Characterization was performed by cell morphology, gene and protein expression. Differentiated cells became elongated and spindle shaped, and towards the end of 6 weeks, sporadic cell aggregation appeared which is typical for smooth muscle cells culture. For gene expression study smooth muscle markers, Alfa smooth muscle actin (ASMA), calponin, and Myocin heavy chain (MHC) were expressed after 3weeks induction. At the protein expression level ASMA, MHC, Smoothelin were expressed after 6 weeks induction. As a conclusion adipose tissue provides a possible source of smooth muscle precursor cells which possess the potential capability for smooth muscle differentiation. This represents a promising alternative for urinary bladder smooth muscle repair.

Original languageEnglish
Pages (from-to)149-153
Number of pages5
JournalMalaysian Journal of Microscopy
Volume9
Issue number1
Publication statusPublished - 1 Jan 2013

Fingerprint

adipose tissues
smooth muscle
muscle cells
stem cells
bladder
Stem cells
regeneration
Smooth Muscle Myocytes
Smooth Muscle
Muscle
Adipose Tissue
Regeneration
Urinary Bladder
Stem Cells
Cells
Tissue
cells
Actins
gene expression
Gene Expression

Keywords

  • Adipose stem cells
  • Bladder wall
  • Muscle cells
  • Tissue engineering

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Instrumentation

Cite this

Human adipose tissue derived stem cells as a source of smooth muscle cells in the regeneration of muscular layer of urinary bladder wall. / Salem, Salah Abood; Hwei, Angela Ng Min; Ho, Chee Kong Christopher; Sagap, Ismail; Idrus, Ruszymah; Zainuddin, Zulkifili M.D.

In: Malaysian Journal of Microscopy, Vol. 9, No. 1, 01.01.2013, p. 149-153.

Research output: Contribution to journalArticle

Salem, Salah Abood ; Hwei, Angela Ng Min ; Ho, Chee Kong Christopher ; Sagap, Ismail ; Idrus, Ruszymah ; Zainuddin, Zulkifili M.D. / Human adipose tissue derived stem cells as a source of smooth muscle cells in the regeneration of muscular layer of urinary bladder wall. In: Malaysian Journal of Microscopy. 2013 ; Vol. 9, No. 1. pp. 149-153.
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