Honey modulates oxidative burst of professional phagocytes

Osman Mesaik Mohammed Ahmed Hassan, M. Kamran Azim, S. Mohiuddin

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The effects of natural honey and its major sugar constituents (i.e. D-fructose, D-glucose, maltose and sucrose) on phagocytic respiratory burst have been studied. Pre-incubated whole blood and isolated leukocytes with honey samples and sugars were induced for phagocytosis and the level of reactive oxygen species (ROS) was monitored by using chemiluminescence assays. Honey samples were found to decrease the luminol-enhanced chemiluminescence in opsonized zymosan-stimulated whole blood and isolated leukocytes with statistically significant differences; indicating inhibition of ROS production including hydrogen peroxide, hydroxyl free radical and hypochlorous acid. Thus honey appears to modify the oxidative burst process by inhibiting phagocytic myeloperoxidase activity. Chemiluminescence assays further showed that among the major sugar constituents of honey, D-fructose in high concentration exerted an inhibitory effect on exocytosis-associated myeloperoxidase catalyzed ROS formation. These results pointed out an immuno-modulatory potential of honey in the course of phagocytosis.

Original languageEnglish
Pages (from-to)1404-1408
Number of pages5
JournalPhytotherapy Research
Volume22
Issue number10
DOIs
Publication statusPublished - Oct 2008
Externally publishedYes

Fingerprint

Respiratory Burst
Honey
Phagocytes
Luminescence
Reactive Oxygen Species
Fructose
Phagocytosis
Peroxidase
Leukocytes
Hypochlorous Acid
Luminol
Zymosan
Maltose
Exocytosis
Hydroxyl Radical
Hydrogen Peroxide
Free Radicals
Sucrose
Glucose

Keywords

  • Chemiluminescence
  • Oxidative burst inhibition
  • Phagocytosis
  • Reactive oxygen species

ASJC Scopus subject areas

  • Pharmacology

Cite this

Honey modulates oxidative burst of professional phagocytes. / Mohammed Ahmed Hassan, Osman Mesaik; Kamran Azim, M.; Mohiuddin, S.

In: Phytotherapy Research, Vol. 22, No. 10, 10.2008, p. 1404-1408.

Research output: Contribution to journalArticle

Mohammed Ahmed Hassan, Osman Mesaik ; Kamran Azim, M. ; Mohiuddin, S. / Honey modulates oxidative burst of professional phagocytes. In: Phytotherapy Research. 2008 ; Vol. 22, No. 10. pp. 1404-1408.
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