Homogeneous solid dispersion (HSD) system for rapid and stable production of succinic acid from lignocellulosic hydrolysate

Abdullah Amru Indera Luthfi, Jian Ping Tan, Shuhaida Harun, Shareena Fairuz Abdul Manaf, Jamaliah Md Jahim

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Continuous bio-production of succinic acid was reported in homogeneous solid dispersion (HSD) system utilizing porous coconut shell activated carbon (CSAC) as immobilization carrier. The aim of the present work was to implement the HSD system to increase the area of cell immobilization and the rate of succinic-acid production from the lignocellulosic medium. The ratio of the two enzymes (cellulase-to-hemicellulase) was initially optimized to break down the lignocellulose into its free monomers, wherein the best ratio was determined as 4:1. Succinic-acid production was evaluated in the HSD system by varying the substrate loading and dilution rate. The results showed that high productivities of succinic acid were obtained when 60 g/L glucose was fed over a dilution rates ranging from 0.03 to 0.4/h. The titer of succinic acid decreased gradually with higher dilution rate, whereas the residual substrate concentration increased with it. Critical dilution rate was determined to be 0.4/h at which the best productivity of succinic acid of 6.58 g/L h and its yield of 0.66 g/g were achieved using oil palm fronds (OPF) hydrolysate. This work lends evidence to the use of CSAC and lignocellulosic hydrolysate to further exploit the potential economies of scale.

Original languageEnglish
JournalBioprocess and Biosystems Engineering
DOIs
Publication statusAccepted/In press - 1 Jan 2018

Fingerprint

Succinic Acid
Dilution
Acids
Cocos
Immobilization
Activated carbon
Carbon
Cell immobilization
Productivity
Palm oil
Cellulase
Substrates
Glucose
Oils
Enzymes
Monomers

Keywords

  • Coconut shell activated carbon
  • Continuous
  • Homogeneous solid dispersion
  • Lignocellulose
  • Succinic acid

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering

Cite this

Homogeneous solid dispersion (HSD) system for rapid and stable production of succinic acid from lignocellulosic hydrolysate. / Luthfi, Abdullah Amru Indera; Tan, Jian Ping; Harun, Shuhaida; Manaf, Shareena Fairuz Abdul; Md Jahim, Jamaliah.

In: Bioprocess and Biosystems Engineering, 01.01.2018.

Research output: Contribution to journalArticle

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AU - Manaf, Shareena Fairuz Abdul

AU - Md Jahim, Jamaliah

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