Homogeneous nature of Malaysian Marine fish Epinephelus fuscoguttatus (Perciformes; Serranidae)

Evidence based on molecular markers, morphology and fourier transform infrared analysis

A'wani Aziz Nurdalila, Bunawan Hamidun, Subbiah Vijay Kumar, Kenneth Francis Rodrigues, Syarul Nataqain Baharum

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Taxonomic confusion exists within the genus Epinephelus due to the lack of morphological specializations and the overwhelming number of species reported in several studies. The homogenous nature of the morphology has created confusion in the Malaysian Marine fish species Epinephelus fuscoguttatus and Epinephelus hexagonatus. In this study, the partial DNA sequence of the 16S gene and mitochondrial nucleotide sequences of two gene regions, Cytochrome Oxidase Subunit I and III were used to investigate the phylogenetic relationship between them. In the phylogenetic trees, E. fuscoguttatus was monophyletic with E. hexagonatus species and morphology examination shows that no significant differences were found in the morphometric features between these two taxa. This suggests that E. fuscoguttatus is not distinguishable from E. hexagonatus species, and that E. fuscoguttatus have been identified to be E. hexagonatus species is likely attributed to differences in environment and ability to camouflage themselves under certain conditions. Interestingly, this finding was also supported by Principal Component Analysis on Attenuated Total Reflectance-Fourier-transform Infrared (ATR-FTIR) data analysis. Molecular, morphological and meristic characteristics were combined with ATR-FTIR analysis used in this study offer new perspectives in fish species identification. To our knowledge, this is the first report of an extensive genetic population study of E. fuscoguttatus in Malaysia and this understanding will play an important role in informing genetic stock-specific strategies for the management and conservation of this highly valued fish.

Original languageEnglish
Pages (from-to)14884-14900
Number of pages17
JournalInternational Journal of Molecular Sciences
Volume16
Issue number7
DOIs
Publication statusPublished - 2 Jul 2007

Fingerprint

Perciformes
Bass
fishes
Fourier Analysis
markers
Fish
Fourier transforms
Fishes
confusion
Infrared radiation
genes
Genes
camouflage
Camouflage
reflectance
Malaysia
Mitochondrial Genes
oxidase
cytochromes
DNA sequences

Keywords

  • 16S
  • Cytochrome oxidase
  • Epinephelus fuscoguttatus
  • Epinephelus hexagonatus
  • Species identification

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Spectroscopy
  • Inorganic Chemistry
  • Catalysis
  • Molecular Biology
  • Computer Science Applications

Cite this

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title = "Homogeneous nature of Malaysian Marine fish Epinephelus fuscoguttatus (Perciformes; Serranidae): Evidence based on molecular markers, morphology and fourier transform infrared analysis",
abstract = "Taxonomic confusion exists within the genus Epinephelus due to the lack of morphological specializations and the overwhelming number of species reported in several studies. The homogenous nature of the morphology has created confusion in the Malaysian Marine fish species Epinephelus fuscoguttatus and Epinephelus hexagonatus. In this study, the partial DNA sequence of the 16S gene and mitochondrial nucleotide sequences of two gene regions, Cytochrome Oxidase Subunit I and III were used to investigate the phylogenetic relationship between them. In the phylogenetic trees, E. fuscoguttatus was monophyletic with E. hexagonatus species and morphology examination shows that no significant differences were found in the morphometric features between these two taxa. This suggests that E. fuscoguttatus is not distinguishable from E. hexagonatus species, and that E. fuscoguttatus have been identified to be E. hexagonatus species is likely attributed to differences in environment and ability to camouflage themselves under certain conditions. Interestingly, this finding was also supported by Principal Component Analysis on Attenuated Total Reflectance-Fourier-transform Infrared (ATR-FTIR) data analysis. Molecular, morphological and meristic characteristics were combined with ATR-FTIR analysis used in this study offer new perspectives in fish species identification. To our knowledge, this is the first report of an extensive genetic population study of E. fuscoguttatus in Malaysia and this understanding will play an important role in informing genetic stock-specific strategies for the management and conservation of this highly valued fish.",
keywords = "16S, Cytochrome oxidase, Epinephelus fuscoguttatus, Epinephelus hexagonatus, Species identification",
author = "Nurdalila, {A'wani Aziz} and Bunawan Hamidun and Kumar, {Subbiah Vijay} and Rodrigues, {Kenneth Francis} and Baharum, {Syarul Nataqain}",
year = "2007",
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T2 - Evidence based on molecular markers, morphology and fourier transform infrared analysis

AU - Nurdalila, A'wani Aziz

AU - Hamidun, Bunawan

AU - Kumar, Subbiah Vijay

AU - Rodrigues, Kenneth Francis

AU - Baharum, Syarul Nataqain

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N2 - Taxonomic confusion exists within the genus Epinephelus due to the lack of morphological specializations and the overwhelming number of species reported in several studies. The homogenous nature of the morphology has created confusion in the Malaysian Marine fish species Epinephelus fuscoguttatus and Epinephelus hexagonatus. In this study, the partial DNA sequence of the 16S gene and mitochondrial nucleotide sequences of two gene regions, Cytochrome Oxidase Subunit I and III were used to investigate the phylogenetic relationship between them. In the phylogenetic trees, E. fuscoguttatus was monophyletic with E. hexagonatus species and morphology examination shows that no significant differences were found in the morphometric features between these two taxa. This suggests that E. fuscoguttatus is not distinguishable from E. hexagonatus species, and that E. fuscoguttatus have been identified to be E. hexagonatus species is likely attributed to differences in environment and ability to camouflage themselves under certain conditions. Interestingly, this finding was also supported by Principal Component Analysis on Attenuated Total Reflectance-Fourier-transform Infrared (ATR-FTIR) data analysis. Molecular, morphological and meristic characteristics were combined with ATR-FTIR analysis used in this study offer new perspectives in fish species identification. To our knowledge, this is the first report of an extensive genetic population study of E. fuscoguttatus in Malaysia and this understanding will play an important role in informing genetic stock-specific strategies for the management and conservation of this highly valued fish.

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