Histomorphometric profile of the corneal response to short-term reverse-geometry orthokeratology lens wear in primate corneas

A pilot study

Pike See Cheah, Mohidin Norhani, Bariah Mohd. Ali, Maung Myint, Munn Sann Lye, Abdul Latiff Azian

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

PURPOSE: To investigate the histological changes in primate cornea induced by short-term overnight orthokeratology (OK). METHODS: Nine young adult primates were used. One animal served as negative control. The remaining 8 animals wore reverse-geometry OK lenses for periods of 4, 8, 16, and 24 hours on 1 eye with the other eye as control. Central and midperipheral corneal thickness, as well as ultrastructural changes in corneal epithelium, stroma and endothelium in response to OK lenses, were evaluated. RESULTS: OK significantly reduced the thickness of the central cornea in all treatment groups. The central corneal thinning was both stromal and epithelial in origin. Substantial midperipheral corneal thickening was seen in 16-hour and 24-hour lens-wear groups and this effect was both stromal and epithelial in origin as well. Histology evidence indicated the primary epithelial response in the central cornea was compression of cells that resulted in wing cells becoming shorter and basal cells being squatted rather than lost or migration of cell layers. These pronounced cell shape changes occurred without compromising the structural integrity of the desmosomes. The thickened corneal epithelium has normal cell layers. The squamous cells have larger surface sizes and are composed of oval instead of flattened nuclei. This implied delayed surface cell exfoliation at the thickened midperipheral epithelium. Physical presence of OK lens over the cornea did not influence the microstructures of microvilli and microplicae, endothelium, and collagen distribution. CONCLUSIONS: The primate cornea, particularly the corneal epithelium, responds rapidly to the application of reverse-geometry OK lenses with significant epithelial cell shape alterations with short-term OK lens wear. This finding suggests that the corneal epithelium is moldable in response to the physical forces generated by the OK lenses.

Original languageEnglish
Pages (from-to)461-470
Number of pages10
JournalCornea
Volume27
Issue number4
DOIs
Publication statusPublished - May 2008

Fingerprint

Cornea
Primates
Lenses
Corneal Epithelium
Cell Shape
Epithelial Cells
Corneal Stroma
Corneal Endothelium
Desmosomes
Microvilli
Endothelium
Cell Movement
Young Adult
Histology
Collagen
Epithelium

Keywords

  • Cornea
  • Histological changes
  • Overnight orthokeratology
  • Primate

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Histomorphometric profile of the corneal response to short-term reverse-geometry orthokeratology lens wear in primate corneas : A pilot study. / Cheah, Pike See; Norhani, Mohidin; Mohd. Ali, Bariah; Myint, Maung; Lye, Munn Sann; Azian, Abdul Latiff.

In: Cornea, Vol. 27, No. 4, 05.2008, p. 461-470.

Research output: Contribution to journalArticle

Cheah, Pike See ; Norhani, Mohidin ; Mohd. Ali, Bariah ; Myint, Maung ; Lye, Munn Sann ; Azian, Abdul Latiff. / Histomorphometric profile of the corneal response to short-term reverse-geometry orthokeratology lens wear in primate corneas : A pilot study. In: Cornea. 2008 ; Vol. 27, No. 4. pp. 461-470.
@article{de416225e193455ebbdfb914f5cabfbc,
title = "Histomorphometric profile of the corneal response to short-term reverse-geometry orthokeratology lens wear in primate corneas: A pilot study",
abstract = "PURPOSE: To investigate the histological changes in primate cornea induced by short-term overnight orthokeratology (OK). METHODS: Nine young adult primates were used. One animal served as negative control. The remaining 8 animals wore reverse-geometry OK lenses for periods of 4, 8, 16, and 24 hours on 1 eye with the other eye as control. Central and midperipheral corneal thickness, as well as ultrastructural changes in corneal epithelium, stroma and endothelium in response to OK lenses, were evaluated. RESULTS: OK significantly reduced the thickness of the central cornea in all treatment groups. The central corneal thinning was both stromal and epithelial in origin. Substantial midperipheral corneal thickening was seen in 16-hour and 24-hour lens-wear groups and this effect was both stromal and epithelial in origin as well. Histology evidence indicated the primary epithelial response in the central cornea was compression of cells that resulted in wing cells becoming shorter and basal cells being squatted rather than lost or migration of cell layers. These pronounced cell shape changes occurred without compromising the structural integrity of the desmosomes. The thickened corneal epithelium has normal cell layers. The squamous cells have larger surface sizes and are composed of oval instead of flattened nuclei. This implied delayed surface cell exfoliation at the thickened midperipheral epithelium. Physical presence of OK lens over the cornea did not influence the microstructures of microvilli and microplicae, endothelium, and collagen distribution. CONCLUSIONS: The primate cornea, particularly the corneal epithelium, responds rapidly to the application of reverse-geometry OK lenses with significant epithelial cell shape alterations with short-term OK lens wear. This finding suggests that the corneal epithelium is moldable in response to the physical forces generated by the OK lenses.",
keywords = "Cornea, Histological changes, Overnight orthokeratology, Primate",
author = "Cheah, {Pike See} and Mohidin Norhani and {Mohd. Ali}, Bariah and Maung Myint and Lye, {Munn Sann} and Azian, {Abdul Latiff}",
year = "2008",
month = "5",
doi = "10.1097/ICO.0b013e318165642c",
language = "English",
volume = "27",
pages = "461--470",
journal = "Cornea",
issn = "0277-3740",
publisher = "Lippincott Williams and Wilkins",
number = "4",

}

TY - JOUR

T1 - Histomorphometric profile of the corneal response to short-term reverse-geometry orthokeratology lens wear in primate corneas

T2 - A pilot study

AU - Cheah, Pike See

AU - Norhani, Mohidin

AU - Mohd. Ali, Bariah

AU - Myint, Maung

AU - Lye, Munn Sann

AU - Azian, Abdul Latiff

PY - 2008/5

Y1 - 2008/5

N2 - PURPOSE: To investigate the histological changes in primate cornea induced by short-term overnight orthokeratology (OK). METHODS: Nine young adult primates were used. One animal served as negative control. The remaining 8 animals wore reverse-geometry OK lenses for periods of 4, 8, 16, and 24 hours on 1 eye with the other eye as control. Central and midperipheral corneal thickness, as well as ultrastructural changes in corneal epithelium, stroma and endothelium in response to OK lenses, were evaluated. RESULTS: OK significantly reduced the thickness of the central cornea in all treatment groups. The central corneal thinning was both stromal and epithelial in origin. Substantial midperipheral corneal thickening was seen in 16-hour and 24-hour lens-wear groups and this effect was both stromal and epithelial in origin as well. Histology evidence indicated the primary epithelial response in the central cornea was compression of cells that resulted in wing cells becoming shorter and basal cells being squatted rather than lost or migration of cell layers. These pronounced cell shape changes occurred without compromising the structural integrity of the desmosomes. The thickened corneal epithelium has normal cell layers. The squamous cells have larger surface sizes and are composed of oval instead of flattened nuclei. This implied delayed surface cell exfoliation at the thickened midperipheral epithelium. Physical presence of OK lens over the cornea did not influence the microstructures of microvilli and microplicae, endothelium, and collagen distribution. CONCLUSIONS: The primate cornea, particularly the corneal epithelium, responds rapidly to the application of reverse-geometry OK lenses with significant epithelial cell shape alterations with short-term OK lens wear. This finding suggests that the corneal epithelium is moldable in response to the physical forces generated by the OK lenses.

AB - PURPOSE: To investigate the histological changes in primate cornea induced by short-term overnight orthokeratology (OK). METHODS: Nine young adult primates were used. One animal served as negative control. The remaining 8 animals wore reverse-geometry OK lenses for periods of 4, 8, 16, and 24 hours on 1 eye with the other eye as control. Central and midperipheral corneal thickness, as well as ultrastructural changes in corneal epithelium, stroma and endothelium in response to OK lenses, were evaluated. RESULTS: OK significantly reduced the thickness of the central cornea in all treatment groups. The central corneal thinning was both stromal and epithelial in origin. Substantial midperipheral corneal thickening was seen in 16-hour and 24-hour lens-wear groups and this effect was both stromal and epithelial in origin as well. Histology evidence indicated the primary epithelial response in the central cornea was compression of cells that resulted in wing cells becoming shorter and basal cells being squatted rather than lost or migration of cell layers. These pronounced cell shape changes occurred without compromising the structural integrity of the desmosomes. The thickened corneal epithelium has normal cell layers. The squamous cells have larger surface sizes and are composed of oval instead of flattened nuclei. This implied delayed surface cell exfoliation at the thickened midperipheral epithelium. Physical presence of OK lens over the cornea did not influence the microstructures of microvilli and microplicae, endothelium, and collagen distribution. CONCLUSIONS: The primate cornea, particularly the corneal epithelium, responds rapidly to the application of reverse-geometry OK lenses with significant epithelial cell shape alterations with short-term OK lens wear. This finding suggests that the corneal epithelium is moldable in response to the physical forces generated by the OK lenses.

KW - Cornea

KW - Histological changes

KW - Overnight orthokeratology

KW - Primate

UR - http://www.scopus.com/inward/record.url?scp=42549137341&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=42549137341&partnerID=8YFLogxK

U2 - 10.1097/ICO.0b013e318165642c

DO - 10.1097/ICO.0b013e318165642c

M3 - Article

VL - 27

SP - 461

EP - 470

JO - Cornea

JF - Cornea

SN - 0277-3740

IS - 4

ER -