Histological study to differentiate between human and non-human long bone

Faridah Mohd. Nor, Robert F. Pastor, Holger Schutkowski

Research output: Contribution to journalArticle

Abstract

Introduction: Determination of bone origin is crucial in forensic investigations, especially when dealing with fragmented skeletal remains. Objective: To examine microstructural differences between human and non-human mammal long bone histology using histomorphometry, and discuss the literature. Method: About 64 deceased persons from the mortuary and 65 animals from the zoo from Southeast Asia were collected. Thin sections from human and non-human bone samples were assessed for the following microstructural parameters i.e. cortical thickness, medullary diameter, osteon count, osteon diameter, osteon area, osteon perimeter, Haversian canal diameter, Haversian canal area, Haversian canal perimeter and Haversian lamella count per osteon using digital image analysis. Result: Discriminant function analysis showed correct classification rates for 81.4% of cases, with identification accuracy of 96.9% for human and 66.2% for non-human (P <0.001). Cross-validation showed correct classification rates for 79.1% of cases with identification accuracy of 95.3% for human and 63.1% for non-human. Conclusion: It is, therefore concluded that microstructural parameters are useful in distinguishing human from non-human bones, and that region-specific equations should be used.

Original languageEnglish
Pages (from-to)413-417
Number of pages5
JournalInternational Medical Journal
Volume22
Issue number5
Publication statusPublished - 2015

Fingerprint

Haversian System
Bone and Bones
Zoo Animals
Southeastern Asia
Discriminant Analysis
Mammals
Histology

Keywords

  • Animal
  • Histomorphometry
  • Human
  • Osteon
  • Southeast Asia

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Histological study to differentiate between human and non-human long bone. / Mohd. Nor, Faridah; Pastor, Robert F.; Schutkowski, Holger.

In: International Medical Journal, Vol. 22, No. 5, 2015, p. 413-417.

Research output: Contribution to journalArticle

Mohd. Nor, Faridah ; Pastor, Robert F. ; Schutkowski, Holger. / Histological study to differentiate between human and non-human long bone. In: International Medical Journal. 2015 ; Vol. 22, No. 5. pp. 413-417.
@article{c45ed9841c704eb4a30497c7e5249000,
title = "Histological study to differentiate between human and non-human long bone",
abstract = "Introduction: Determination of bone origin is crucial in forensic investigations, especially when dealing with fragmented skeletal remains. Objective: To examine microstructural differences between human and non-human mammal long bone histology using histomorphometry, and discuss the literature. Method: About 64 deceased persons from the mortuary and 65 animals from the zoo from Southeast Asia were collected. Thin sections from human and non-human bone samples were assessed for the following microstructural parameters i.e. cortical thickness, medullary diameter, osteon count, osteon diameter, osteon area, osteon perimeter, Haversian canal diameter, Haversian canal area, Haversian canal perimeter and Haversian lamella count per osteon using digital image analysis. Result: Discriminant function analysis showed correct classification rates for 81.4{\%} of cases, with identification accuracy of 96.9{\%} for human and 66.2{\%} for non-human (P <0.001). Cross-validation showed correct classification rates for 79.1{\%} of cases with identification accuracy of 95.3{\%} for human and 63.1{\%} for non-human. Conclusion: It is, therefore concluded that microstructural parameters are useful in distinguishing human from non-human bones, and that region-specific equations should be used.",
keywords = "Animal, Histomorphometry, Human, Osteon, Southeast Asia",
author = "{Mohd. Nor}, Faridah and Pastor, {Robert F.} and Holger Schutkowski",
year = "2015",
language = "English",
volume = "22",
pages = "413--417",
journal = "International Medical Journal",
issn = "1341-2051",
publisher = "Japan International Cultural Exchange Foundation",
number = "5",

}

TY - JOUR

T1 - Histological study to differentiate between human and non-human long bone

AU - Mohd. Nor, Faridah

AU - Pastor, Robert F.

AU - Schutkowski, Holger

PY - 2015

Y1 - 2015

N2 - Introduction: Determination of bone origin is crucial in forensic investigations, especially when dealing with fragmented skeletal remains. Objective: To examine microstructural differences between human and non-human mammal long bone histology using histomorphometry, and discuss the literature. Method: About 64 deceased persons from the mortuary and 65 animals from the zoo from Southeast Asia were collected. Thin sections from human and non-human bone samples were assessed for the following microstructural parameters i.e. cortical thickness, medullary diameter, osteon count, osteon diameter, osteon area, osteon perimeter, Haversian canal diameter, Haversian canal area, Haversian canal perimeter and Haversian lamella count per osteon using digital image analysis. Result: Discriminant function analysis showed correct classification rates for 81.4% of cases, with identification accuracy of 96.9% for human and 66.2% for non-human (P <0.001). Cross-validation showed correct classification rates for 79.1% of cases with identification accuracy of 95.3% for human and 63.1% for non-human. Conclusion: It is, therefore concluded that microstructural parameters are useful in distinguishing human from non-human bones, and that region-specific equations should be used.

AB - Introduction: Determination of bone origin is crucial in forensic investigations, especially when dealing with fragmented skeletal remains. Objective: To examine microstructural differences between human and non-human mammal long bone histology using histomorphometry, and discuss the literature. Method: About 64 deceased persons from the mortuary and 65 animals from the zoo from Southeast Asia were collected. Thin sections from human and non-human bone samples were assessed for the following microstructural parameters i.e. cortical thickness, medullary diameter, osteon count, osteon diameter, osteon area, osteon perimeter, Haversian canal diameter, Haversian canal area, Haversian canal perimeter and Haversian lamella count per osteon using digital image analysis. Result: Discriminant function analysis showed correct classification rates for 81.4% of cases, with identification accuracy of 96.9% for human and 66.2% for non-human (P <0.001). Cross-validation showed correct classification rates for 79.1% of cases with identification accuracy of 95.3% for human and 63.1% for non-human. Conclusion: It is, therefore concluded that microstructural parameters are useful in distinguishing human from non-human bones, and that region-specific equations should be used.

KW - Animal

KW - Histomorphometry

KW - Human

KW - Osteon

KW - Southeast Asia

UR - http://www.scopus.com/inward/record.url?scp=84949496588&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84949496588&partnerID=8YFLogxK

M3 - Article

VL - 22

SP - 413

EP - 417

JO - International Medical Journal

JF - International Medical Journal

SN - 1341-2051

IS - 5

ER -