Histological changes in the fracture callus following the administration of water extract of Piper sarmentosum (Daun Kadok) in estrogen-deficient rats

Mohamed Abdalla Estai, Ima Nirwana Soelaiman, Ahmad Nazrun Shuid, Srijit Das, Aishah Mohd Ali, Farihah Suhaimi

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12 Citations (Scopus)

Abstract

Background: The fracture healing is impaired in osteoporosis. Piper sarmentosum is a plant, which contains potent antioxidant, naringenin that may enhance fracture healing. The present histological study aimed to determine the effects of water extract of Piper sarmentosum on the late phase of fracture healing in estrogen-deficient rats. Methods: Twenty four female Sprague-Dawley rats (200-250 gm) were obtained. Six rats underwent sham operation and the rest were ovariectomized. Six weeks post-ovariectomy all the rats were fractured at the mid-diaphysis of the right femur and a K-wire was inserted for internal fixation. The sham group was given vehicle (normal saline) and the ovariectomized group was randomly subdivided into three groups: (i) ovariectomized-control group supplemented with vehicle; (ii) ovariectomized+ estrogen replacement therapy group treated with estrogen (100 μg/kg/day) and (iii) ovariectomized+Piper sarmentosum group treated with Piper sarmentosum water extract (125 mg/kg). Following six weeks of treatment, the rats were sacrificed and the right femora were harvested for histological assessment of fracture callus. Results: The ovariectomized-control group showed a significant delay in fracture healing compared to the sham, ovariectomized-estrogen replacement therapy and ovariectomized-Piper sarmentosum groups. The median callus score for the ovariectomized-Piper sarmentosum group was 4.50 (range, 4-5), which was significantly higher than the median callus score 3.50 (range, 3-4) for the ovariectomized-control group (P=0.019). However, there was no significant (P>0.05) difference in the callus score among the sham, ovariectomizedestrogen replacement therapy and ovariectomized-Piper sarmentosum groups groups. Conclusion: Treatment with water extract of Piper sarmentosum proved beneficial in the fracture healing in estrogendeficient rats.

Original languageEnglish
Pages (from-to)281-288
Number of pages8
JournalIranian Journal of Medical Sciences
Volume36
Issue number4
Publication statusPublished - Dec 2011

Fingerprint

Piper
Bony Callus
Estrogens
Fracture Healing
Water
Estrogen Replacement Therapy
Femur
Control Groups
Diaphyses
Water Purification
Ovariectomy
Osteoporosis
Sprague Dawley Rats
Antioxidants

Keywords

  • Antioxidant
  • Callus
  • Fracture healing
  • Histology
  • Osteoporosis
  • Ovariectomy

ASJC Scopus subject areas

  • Medicine(all)

Cite this

@article{61a51bc60e214ea88fa103ce747e3dbc,
title = "Histological changes in the fracture callus following the administration of water extract of Piper sarmentosum (Daun Kadok) in estrogen-deficient rats",
abstract = "Background: The fracture healing is impaired in osteoporosis. Piper sarmentosum is a plant, which contains potent antioxidant, naringenin that may enhance fracture healing. The present histological study aimed to determine the effects of water extract of Piper sarmentosum on the late phase of fracture healing in estrogen-deficient rats. Methods: Twenty four female Sprague-Dawley rats (200-250 gm) were obtained. Six rats underwent sham operation and the rest were ovariectomized. Six weeks post-ovariectomy all the rats were fractured at the mid-diaphysis of the right femur and a K-wire was inserted for internal fixation. The sham group was given vehicle (normal saline) and the ovariectomized group was randomly subdivided into three groups: (i) ovariectomized-control group supplemented with vehicle; (ii) ovariectomized+ estrogen replacement therapy group treated with estrogen (100 μg/kg/day) and (iii) ovariectomized+Piper sarmentosum group treated with Piper sarmentosum water extract (125 mg/kg). Following six weeks of treatment, the rats were sacrificed and the right femora were harvested for histological assessment of fracture callus. Results: The ovariectomized-control group showed a significant delay in fracture healing compared to the sham, ovariectomized-estrogen replacement therapy and ovariectomized-Piper sarmentosum groups. The median callus score for the ovariectomized-Piper sarmentosum group was 4.50 (range, 4-5), which was significantly higher than the median callus score 3.50 (range, 3-4) for the ovariectomized-control group (P=0.019). However, there was no significant (P>0.05) difference in the callus score among the sham, ovariectomizedestrogen replacement therapy and ovariectomized-Piper sarmentosum groups groups. Conclusion: Treatment with water extract of Piper sarmentosum proved beneficial in the fracture healing in estrogendeficient rats.",
keywords = "Antioxidant, Callus, Fracture healing, Histology, Osteoporosis, Ovariectomy",
author = "Estai, {Mohamed Abdalla} and Soelaiman, {Ima Nirwana} and Shuid, {Ahmad Nazrun} and Srijit Das and Ali, {Aishah Mohd} and Farihah Suhaimi",
year = "2011",
month = "12",
language = "English",
volume = "36",
pages = "281--288",
journal = "Iranian Journal of Medical Sciences",
issn = "0253-0716",
publisher = "Shiraz University of Medical Sciences",
number = "4",

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T1 - Histological changes in the fracture callus following the administration of water extract of Piper sarmentosum (Daun Kadok) in estrogen-deficient rats

AU - Estai, Mohamed Abdalla

AU - Soelaiman, Ima Nirwana

AU - Shuid, Ahmad Nazrun

AU - Das, Srijit

AU - Ali, Aishah Mohd

AU - Suhaimi, Farihah

PY - 2011/12

Y1 - 2011/12

N2 - Background: The fracture healing is impaired in osteoporosis. Piper sarmentosum is a plant, which contains potent antioxidant, naringenin that may enhance fracture healing. The present histological study aimed to determine the effects of water extract of Piper sarmentosum on the late phase of fracture healing in estrogen-deficient rats. Methods: Twenty four female Sprague-Dawley rats (200-250 gm) were obtained. Six rats underwent sham operation and the rest were ovariectomized. Six weeks post-ovariectomy all the rats were fractured at the mid-diaphysis of the right femur and a K-wire was inserted for internal fixation. The sham group was given vehicle (normal saline) and the ovariectomized group was randomly subdivided into three groups: (i) ovariectomized-control group supplemented with vehicle; (ii) ovariectomized+ estrogen replacement therapy group treated with estrogen (100 μg/kg/day) and (iii) ovariectomized+Piper sarmentosum group treated with Piper sarmentosum water extract (125 mg/kg). Following six weeks of treatment, the rats were sacrificed and the right femora were harvested for histological assessment of fracture callus. Results: The ovariectomized-control group showed a significant delay in fracture healing compared to the sham, ovariectomized-estrogen replacement therapy and ovariectomized-Piper sarmentosum groups. The median callus score for the ovariectomized-Piper sarmentosum group was 4.50 (range, 4-5), which was significantly higher than the median callus score 3.50 (range, 3-4) for the ovariectomized-control group (P=0.019). However, there was no significant (P>0.05) difference in the callus score among the sham, ovariectomizedestrogen replacement therapy and ovariectomized-Piper sarmentosum groups groups. Conclusion: Treatment with water extract of Piper sarmentosum proved beneficial in the fracture healing in estrogendeficient rats.

AB - Background: The fracture healing is impaired in osteoporosis. Piper sarmentosum is a plant, which contains potent antioxidant, naringenin that may enhance fracture healing. The present histological study aimed to determine the effects of water extract of Piper sarmentosum on the late phase of fracture healing in estrogen-deficient rats. Methods: Twenty four female Sprague-Dawley rats (200-250 gm) were obtained. Six rats underwent sham operation and the rest were ovariectomized. Six weeks post-ovariectomy all the rats were fractured at the mid-diaphysis of the right femur and a K-wire was inserted for internal fixation. The sham group was given vehicle (normal saline) and the ovariectomized group was randomly subdivided into three groups: (i) ovariectomized-control group supplemented with vehicle; (ii) ovariectomized+ estrogen replacement therapy group treated with estrogen (100 μg/kg/day) and (iii) ovariectomized+Piper sarmentosum group treated with Piper sarmentosum water extract (125 mg/kg). Following six weeks of treatment, the rats were sacrificed and the right femora were harvested for histological assessment of fracture callus. Results: The ovariectomized-control group showed a significant delay in fracture healing compared to the sham, ovariectomized-estrogen replacement therapy and ovariectomized-Piper sarmentosum groups. The median callus score for the ovariectomized-Piper sarmentosum group was 4.50 (range, 4-5), which was significantly higher than the median callus score 3.50 (range, 3-4) for the ovariectomized-control group (P=0.019). However, there was no significant (P>0.05) difference in the callus score among the sham, ovariectomizedestrogen replacement therapy and ovariectomized-Piper sarmentosum groups groups. Conclusion: Treatment with water extract of Piper sarmentosum proved beneficial in the fracture healing in estrogendeficient rats.

KW - Antioxidant

KW - Callus

KW - Fracture healing

KW - Histology

KW - Osteoporosis

KW - Ovariectomy

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