High-yield purification of an organic solvent-tolerant lipase from Pseudomonas sp. strain S5

Raja Noor Zaliha R A Rahman, Syarul Nataqain Baharum, Mahiran Basri, Abu Bakar Salleh

Research output: Contribution to journalArticle

109 Citations (Scopus)

Abstract

An organic solvent-tolerant S5 lipase was purified by affinity chromatography and anion exchange chromatography. The molecular mass of the lipase was estimated to be 60 kDa with 387 purification fold. The optimal temperature and pH were 45°C and 9.0, respectively. The purified lipase was stable at 45°C and pH 6-9. It exhibited the highest stability in the presence of various organic solvents such as n-dodecane, 1-pentanol, and toluene. Ca2+ and Mg2+ stimulated lipase activity, whereas EDTA had no effect on its activity. The S5 lipase exhibited the highest activity in the presence of palm oil as a natural oil and triolein as a synthetic triglyceride. It showed random positional specificity on the thin-layer chromatography.

Original languageEnglish
Pages (from-to)267-274
Number of pages8
JournalAnalytical Biochemistry
Volume341
Issue number2
DOIs
Publication statusPublished - 15 Jun 2005
Externally publishedYes

Fingerprint

Pseudomonas
Lipase
Organic solvents
Purification
Triolein
Affinity chromatography
Thin layer chromatography
Toluene
Molecular mass
Thin Layer Chromatography
Chromatography
Affinity Chromatography
Edetic Acid
Anions
Oils
Triglycerides
Temperature

Keywords

  • Characterization
  • Organic solvent-tolerant lipase
  • Pseudomonas sp. strain S5
  • Purification

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

High-yield purification of an organic solvent-tolerant lipase from Pseudomonas sp. strain S5. / Rahman, Raja Noor Zaliha R A; Baharum, Syarul Nataqain; Basri, Mahiran; Salleh, Abu Bakar.

In: Analytical Biochemistry, Vol. 341, No. 2, 15.06.2005, p. 267-274.

Research output: Contribution to journalArticle

Rahman, Raja Noor Zaliha R A ; Baharum, Syarul Nataqain ; Basri, Mahiran ; Salleh, Abu Bakar. / High-yield purification of an organic solvent-tolerant lipase from Pseudomonas sp. strain S5. In: Analytical Biochemistry. 2005 ; Vol. 341, No. 2. pp. 267-274.
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