High xylooligosaccharides (XOS) production from pretreated kenaf stem by enzyme mixture hydrolysis

Nur Izyan Wan Azelee, Jamaliah Md Jahim, Ahmad Fauzi Ismail, Siti Fatimah Zaharah Mohamad Fuzi, Roshanida A. Rahman, Rosli Md Illias

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Advancement in industrial biotechnology offers potential opportunities for economic utilization of agro-industrial biomass such as kenaf (Hibiscus cannabinus) for the production of value-added products. This work was conducted to evaluate an enzymatic hydrolysis of kenaf stem (that has been pretreated) for xylooligosaccharides (XOS) production. Pretreated kenaf stems were subjected to two enzymatic hydrolysis approaches which are single xylanase (Xyn2) and a mixture of xylanase:arabinofuranosidase (Xyn2:An. abfA). Central Composite Design was used to optimize the enzymatic hydrolysis conditions on the pretreated kenaf stem to achieve maximum hemicellulose conversion. Under the optimized hydrolysis conditions, hemicellulose conversion (95.03%) with the highest XOS yield of 351.46 mg/g was achieved at 40 °C (pH 4.0) after 48. h incubation with Xyn2:An. abfA ratio of 400. U:10. U. Xylobiose (135.42 mg/g) is the main XOS product with xylotriose (102.04 mg/g) being the second highest. The high production of XOS yield from the pretreated kenaf stem demonstrated the biotechnological potential of the kenaf in the future.

Original languageEnglish
Pages (from-to)11-19
Number of pages9
JournalIndustrial Crops and Products
Volume81
DOIs
Publication statusPublished - 1 Mar 2016

Fingerprint

xylooligosaccharides
kenaf
hydrolysis
stems
xylanases
enzymatic hydrolysis
enzymes
alpha-N-arabinofuranosidase
hemicellulose
Hibiscus cannabinus
value-added products
biotechnology
economics
biomass

Keywords

  • Arabinofuranosidase
  • Enzyme mixture
  • Hemicellulose
  • Xylanase
  • Xylooligosaccharides

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

High xylooligosaccharides (XOS) production from pretreated kenaf stem by enzyme mixture hydrolysis. / Wan Azelee, Nur Izyan; Md Jahim, Jamaliah; Ismail, Ahmad Fauzi; Fuzi, Siti Fatimah Zaharah Mohamad; Rahman, Roshanida A.; Md Illias, Rosli.

In: Industrial Crops and Products, Vol. 81, 01.03.2016, p. 11-19.

Research output: Contribution to journalArticle

Wan Azelee, Nur Izyan ; Md Jahim, Jamaliah ; Ismail, Ahmad Fauzi ; Fuzi, Siti Fatimah Zaharah Mohamad ; Rahman, Roshanida A. ; Md Illias, Rosli. / High xylooligosaccharides (XOS) production from pretreated kenaf stem by enzyme mixture hydrolysis. In: Industrial Crops and Products. 2016 ; Vol. 81. pp. 11-19.
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