High prevalence of toxigenic and nontoxigenic clostridium difficile strains in Malaysia

Thomas V. Riley, Deirdre A. Collins, Rina Karunakaran, Maria Abdul Kahar, Ariza Adnan, Siti Asma Hassan, Nadiah Hanim Zainul, F. R.Mohammed Rustam, Z. Abd Wahab, Ramliza Ramli, Yeong Yeh Lee, Hamimah Hassan

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Accumulating evidence shows a high prevalence of Clostridium difficile in Southeast Asia associated with a range of clinical presentations. However, severe infections are rarely reported. We investigated C. difficile infection (CDI) across four hospitals in Kuala Lumpur and Kota Bharu, Malaysia. Enzyme immunoassays for glutamate dehydrogenase (GDH) and toxin A or B were performed on diarrheal stool specimens collected from patients in 2015 and 2016. Specimens were also cultured and isolates of C. difficile characterized by PCR ribotyping and detection of toxin genes. In total, 437 specimens were collected and fecal toxin was detected in 3.0%. A further 16.2% of specimens were GDH positive and toxin negative. After culture, toxigenic strains were isolated from 10.3% and nontoxigenic strains from 12.4% of specimens. The most prevalent PCR ribotypes (RTs) were RT 017 (20.0%) and RT 043 (10.0%). The high prevalence of RT 017 and nontoxigenic strains in Malaysia and in neighboring Thailand and Indonesia suggests that they localize to the region of Southeast Asia, with an implication that they may mediate the burden of CDI in the region.

Original languageEnglish
JournalJournal of Clinical Microbiology
Volume56
Issue number6
DOIs
Publication statusPublished - 1 Jun 2018

Fingerprint

Ribotyping
Clostridium difficile
Malaysia
Clostridium Infections
Glutamate Dehydrogenase
Southeastern Asia
Polymerase Chain Reaction
Indonesia
Thailand
Immunoenzyme Techniques
Infection
Genes

Keywords

  • Clostridium difficile
  • Epidemiology
  • Malaysia
  • Prevalence

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Riley, T. V., Collins, D. A., Karunakaran, R., Kahar, M. A., Adnan, A., Hassan, S. A., ... Hassan, H. (2018). High prevalence of toxigenic and nontoxigenic clostridium difficile strains in Malaysia. Journal of Clinical Microbiology, 56(6). https://doi.org/10.1128/JCM.00170-18

High prevalence of toxigenic and nontoxigenic clostridium difficile strains in Malaysia. / Riley, Thomas V.; Collins, Deirdre A.; Karunakaran, Rina; Kahar, Maria Abdul; Adnan, Ariza; Hassan, Siti Asma; Zainul, Nadiah Hanim; Rustam, F. R.Mohammed; Wahab, Z. Abd; Ramli, Ramliza; Lee, Yeong Yeh; Hassan, Hamimah.

In: Journal of Clinical Microbiology, Vol. 56, No. 6, 01.06.2018.

Research output: Contribution to journalArticle

Riley, TV, Collins, DA, Karunakaran, R, Kahar, MA, Adnan, A, Hassan, SA, Zainul, NH, Rustam, FRM, Wahab, ZA, Ramli, R, Lee, YY & Hassan, H 2018, 'High prevalence of toxigenic and nontoxigenic clostridium difficile strains in Malaysia', Journal of Clinical Microbiology, vol. 56, no. 6. https://doi.org/10.1128/JCM.00170-18
Riley, Thomas V. ; Collins, Deirdre A. ; Karunakaran, Rina ; Kahar, Maria Abdul ; Adnan, Ariza ; Hassan, Siti Asma ; Zainul, Nadiah Hanim ; Rustam, F. R.Mohammed ; Wahab, Z. Abd ; Ramli, Ramliza ; Lee, Yeong Yeh ; Hassan, Hamimah. / High prevalence of toxigenic and nontoxigenic clostridium difficile strains in Malaysia. In: Journal of Clinical Microbiology. 2018 ; Vol. 56, No. 6.
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