High prevalence of pfcrt K76T mutants among plasmodium falciparum isolates from Sabah, Malaysia

Nor Azrina Norahmad, Noor Rain Abdullah, Norhayati Yaccob, Jenarun Jelip, Jiloris F. Dony, Khairul Faiz Ruslan, Lokman Hakim Sulaiman, Hasidah Mohd. Sidek, Harald Noedl, Zakiah Ismail

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Chloroquine (CQ) remains the first line drug for the prevention and treatment of malaria in Malaysia in spite of the fact that resistance to CQ has been observed in Malaysia since the 1960s. CQ-resistance is associated with various mutations in pfcrt, which encodes a putative transporter located in the digestive vacuolar membrane of P. falciparum. Substitution of lysine (K) to threonine (T) at amino acid 76 (K76T) in pfcrt is the primary genetic marker conferring resistance to CQ. To determine the presence of T76 mutation in pfcrt from selected areas of Kalabakan, Malaysia 619 blood samples were screened for P. falciparum, out of which 31 were positive. Blood samples were collected on 3 MM ® Whatman filter papers and DNA was extracted using QIAmp DNA mini kit. RFLP-PCR for the detection of the CQ-resistant T76 and sensitive K76 genotype was carried out. Twenty-five samples were shown to have the point mutation in pfcrt whereas the remaining samples were classified as CQ-sensitive (wild-type). In view of the fact that CQ is the first line anti-malarial drug in Malaysia, this finding could be an important indication that treatment with CQ may no longer be effective in the future.

Original languageEnglish
Pages (from-to)1322-1326
Number of pages5
JournalSoutheast Asian Journal of Tropical Medicine and Public Health
Volume42
Issue number6
Publication statusPublished - 2011

Fingerprint

Malaysia
Chloroquine
Plasmodium falciparum
Mutation
DNA
Antimalarials
Threonine
Genetic Markers
Point Mutation
Restriction Fragment Length Polymorphisms
Pharmaceutical Preparations
Malaria
Lysine
Genotype
Amino Acids
Polymerase Chain Reaction
Membranes

Keywords

  • Chloroquine resistant
  • Pfcrt
  • Plasmodium falciparum

ASJC Scopus subject areas

  • Infectious Diseases
  • Public Health, Environmental and Occupational Health

Cite this

Norahmad, N. A., Abdullah, N. R., Yaccob, N., Jelip, J., Dony, J. F., Ruslan, K. F., ... Ismail, Z. (2011). High prevalence of pfcrt K76T mutants among plasmodium falciparum isolates from Sabah, Malaysia. Southeast Asian Journal of Tropical Medicine and Public Health, 42(6), 1322-1326.

High prevalence of pfcrt K76T mutants among plasmodium falciparum isolates from Sabah, Malaysia. / Norahmad, Nor Azrina; Abdullah, Noor Rain; Yaccob, Norhayati; Jelip, Jenarun; Dony, Jiloris F.; Ruslan, Khairul Faiz; Sulaiman, Lokman Hakim; Mohd. Sidek, Hasidah; Noedl, Harald; Ismail, Zakiah.

In: Southeast Asian Journal of Tropical Medicine and Public Health, Vol. 42, No. 6, 2011, p. 1322-1326.

Research output: Contribution to journalArticle

Norahmad, NA, Abdullah, NR, Yaccob, N, Jelip, J, Dony, JF, Ruslan, KF, Sulaiman, LH, Mohd. Sidek, H, Noedl, H & Ismail, Z 2011, 'High prevalence of pfcrt K76T mutants among plasmodium falciparum isolates from Sabah, Malaysia', Southeast Asian Journal of Tropical Medicine and Public Health, vol. 42, no. 6, pp. 1322-1326.
Norahmad, Nor Azrina ; Abdullah, Noor Rain ; Yaccob, Norhayati ; Jelip, Jenarun ; Dony, Jiloris F. ; Ruslan, Khairul Faiz ; Sulaiman, Lokman Hakim ; Mohd. Sidek, Hasidah ; Noedl, Harald ; Ismail, Zakiah. / High prevalence of pfcrt K76T mutants among plasmodium falciparum isolates from Sabah, Malaysia. In: Southeast Asian Journal of Tropical Medicine and Public Health. 2011 ; Vol. 42, No. 6. pp. 1322-1326.
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