High-level expression of bovine rotavirus VP4 protein in Escherichia coli using a hepatitis B core antigen vector

Nazlina Ibrahim, Malcolm A. McCrae

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Six regions of the VP4 protein of bovine rotavirus strain UKtc were expressed using hepatitis B core antigen () as a carrier. Following induction by IPTG, the six fusion proteins, AHBcAg through FHBcAg, were expressed in Escherichia coli to a level of 20-37% of total cellular protein. Soluble fusion proteins in a particulate form were partially purified with yields ranging from 0.5-6.4 mg 1-1 of culture.

Original languageEnglish
Pages (from-to)643-646
Number of pages4
JournalBiotechnology Letters
Volume23
Issue number8
DOIs
Publication statusPublished - 2001

Fingerprint

Hepatitis B Core Antigens
Antigens
Escherichia coli
Proteins
Isopropyl Thiogalactoside
Fusion reactions
Rotavirus VP4 protein

Keywords

  • Escherichia coli, expression, hepatitis B core antigen-VP4 fusion proteins

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Microbiology
  • Bioengineering

Cite this

High-level expression of bovine rotavirus VP4 protein in Escherichia coli using a hepatitis B core antigen vector. / Ibrahim, Nazlina; McCrae, Malcolm A.

In: Biotechnology Letters, Vol. 23, No. 8, 2001, p. 643-646.

Research output: Contribution to journalArticle

@article{964c095968e247528fd3fea2b9ad8e46,
title = "High-level expression of bovine rotavirus VP4 protein in Escherichia coli using a hepatitis B core antigen vector",
abstract = "Six regions of the VP4 protein of bovine rotavirus strain UKtc were expressed using hepatitis B core antigen () as a carrier. Following induction by IPTG, the six fusion proteins, AHBcAg through FHBcAg, were expressed in Escherichia coli to a level of 20-37{\%} of total cellular protein. Soluble fusion proteins in a particulate form were partially purified with yields ranging from 0.5-6.4 mg 1-1 of culture.",
keywords = "Escherichia coli, expression, hepatitis B core antigen-VP4 fusion proteins",
author = "Nazlina Ibrahim and McCrae, {Malcolm A.}",
year = "2001",
doi = "10.1023/A:1010310232033",
language = "English",
volume = "23",
pages = "643--646",
journal = "Biotechnology Letters",
issn = "0141-5492",
publisher = "Springer Netherlands",
number = "8",

}

TY - JOUR

T1 - High-level expression of bovine rotavirus VP4 protein in Escherichia coli using a hepatitis B core antigen vector

AU - Ibrahim, Nazlina

AU - McCrae, Malcolm A.

PY - 2001

Y1 - 2001

N2 - Six regions of the VP4 protein of bovine rotavirus strain UKtc were expressed using hepatitis B core antigen () as a carrier. Following induction by IPTG, the six fusion proteins, AHBcAg through FHBcAg, were expressed in Escherichia coli to a level of 20-37% of total cellular protein. Soluble fusion proteins in a particulate form were partially purified with yields ranging from 0.5-6.4 mg 1-1 of culture.

AB - Six regions of the VP4 protein of bovine rotavirus strain UKtc were expressed using hepatitis B core antigen () as a carrier. Following induction by IPTG, the six fusion proteins, AHBcAg through FHBcAg, were expressed in Escherichia coli to a level of 20-37% of total cellular protein. Soluble fusion proteins in a particulate form were partially purified with yields ranging from 0.5-6.4 mg 1-1 of culture.

KW - Escherichia coli, expression, hepatitis B core antigen-VP4 fusion proteins

UR - http://www.scopus.com/inward/record.url?scp=0035028883&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035028883&partnerID=8YFLogxK

U2 - 10.1023/A:1010310232033

DO - 10.1023/A:1010310232033

M3 - Article

AN - SCOPUS:0035028883

VL - 23

SP - 643

EP - 646

JO - Biotechnology Letters

JF - Biotechnology Letters

SN - 0141-5492

IS - 8

ER -